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We narrowed to 816 results for: ache

Showing: 541 - 560 of 816 results

  2. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ... communication about experimental methods and approaches. It’s very easy to think that the methods section...increase dramatically. I’m not sure what the best approach to doing that will be, and it will need some effort...

  3. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ..., Thoru Pederson’s lab at the University of Massachusetts Medical School developed a dCas9-based multicolor...hairpin sequences (either MS2, PP7, or boxB) that each recruits a different fluorescent protein (BFP, GFP...

  5. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...of sgRNA libraries, with maximum complexity, is achieved with synthetic oligos with a randomized region...associated with otherwise more complicated assembly approaches, and opens the door for new opportunities in ...

  6. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ... specificity and resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat... GFP fused to the SunTag-specific antibody scFv. Each time the reporter is translated, the SunTag sites...

  7. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...over several millennia as their paths cross with each other and with the events of the times."     - Aliyah...non-scientists. It wouldn’t matter whether you had a bachelor’s degree or a PhD, majored in Biochemistry or International...

  8. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...between peak fluorescence and resting fluorescence (achieved through lowering sensor resting fluorescence, ...genetically-defined neuronal populations with intersectional approaches is a growing field that we aim to support by ...

  9. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...drives better after a few drinks. A family member approached me for advice on her baby’s health but adamantly...self-medicate with when they get a cold. A primary school teacher proudly boasted to me that he never eats “anything...

  10. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...combative, field. Of course they are competing with each other, but it's a healthy competition. What an honor...Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts in the Microbiology Community Sign Up ...

  11. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...this question led us to develop a new, rational approach to improve the efficiency of sequence replacement... order of magnitude greater than transfection approaches. Moreover, the cost of reagents is relatively...

  12. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...cells. Positive selection in mammalian cells To achieve stable transfection, there should be a selective...required for efficient selection. This is usually achieved by performing a "kill curve" (basically growing...

  13. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...usable product with two 5′ overhangs, so these approaches require higher concentrations of insert to succeed...multicomponent assembly have 5′ or 3′ sequence homology to each other, they may be assembled incorrectly. To overcome...

  14. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...replicate episomally, essentially remaining an extrachromosomal part of the host genome that can replicate...j.gendis.2017.04.001 Maetzig T, Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology...

  16. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...tracking the reaction progress as in real time PCR, each reaction partition is individually assessed for ...The amount of both mutated and wild type target in each sample can be quickly calculated (details in [2]...

  17. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...suggestions for using LinkedIn and Facebook. For each of the three social media platforms, there’s some...blog writing tips are elements I try to include in each post I write for the Addgene blog. 5. "Social Media...

  18. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...three types of inducible promoters, and examples of each type. Many of these promoter systems are available...promoter. Experimental System Because transcription machinery differs between cell types or organisms, promoters...

  19. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...slides, learn from audiences as they do science outreach and engage with the public, and learn from the...science communication to more graduate students.   Each June, graduate student leaders in science communication...
Showing: 541 - 560 of 816 results