We narrowed to 837 results for: ache

... array is transcribed and processed into crRNAs, each including one repeat and one spacer. In bacteria...protospacers. Repeats: Bacterial DNA that appears between each spacer in the CRISPR array, distinguishing the foreign...

...This post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share ...Bairoch from the Swiss Institute of Bioinformatics reached out to us to tell us about a resource called Cellosaurus...

...could provide a streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind...prime editing with a site-specific recombinase to achieve insertion of long sequences. In their experiments...

...specific components that make up a plasmid and why each is important. Our “Plasmids 101” series designed...replication within a plasmid by recruiting replication machinery proteins Antibiotic Resistance Gene Allows for...

...stand clearly and routinely. They are clear with each employee about what they do well and where they ...reading try The Discipline of Teams by Jon R. Katzenbach and Douglas K. Smith or the book on which this...

...analog versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also discussed...

... Your PI should be the kind of mentor that will teach you how to: interpret data, solve research problems...to ensure the PI will be a good role model and teacher. Do you admire her management style? Does he have...

...control data for each of the materials we distribute. Quality control info can be found on each viral prep’...

...for science’s sake More opportunity to train and teach students Collaborations may be less “complicated...concentrated to business hours (mostly), but can often achieve more structured time management conducive to work...

...on track to succeed at the same time. I began approaching science funding organizations to request support...all labs sharing materials via Addgene. Perhaps if each of many grantors supporting research could provide...

...Chemogenetics Bryan Roth New serotype pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato AAV8 Molecular tool Massimo Scanziani...and resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  More resources on the...

...way through a series of obstacles in pursuit of achieving knowledge. Or, as we in the storytelling business...  Rachel Leeson...

...been extracted from a cell, instead of when it is attached to a cell membrane.  Picking the Right Antibody... Rachel Leeson...

...quantitative assays such as antibody specific activity for each reagent batch. To supplement this, Addgene will ...typically takes a few years for a new project to reach a sufficient number of distributions to cover costs...

...began in high school. “I had a great chemistry teacher in 10th grade and then fell in love with science...HMS) where he leads a lab of 25 people. He also teaches at HMS and has been involved in a variety of commercialization...

...fund is an impossibility at our early stage.  After each pitch we made, we considered the feedback and adjusted... well and trust.  This will allow you to discuss each project and business decision before making it, ...

...Creating a modular, selection-based gene tagging approach Multiple features of C. elegans have made CRISPR...Additional Resources on the Addgene Blog An “elegans” Approach to Better Cas9 Editing Efficiency CRISPR 101: ...

...combative, field. Of course they are competing with each other, but it's a healthy competition. What an honor...Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts in the Microbiology Community Sign Up ...

...this question led us to develop a new, rational approach to improve the efficiency of sequence replacement... order of magnitude greater than transfection approaches. Moreover, the cost of reagents is relatively...

...of sgRNA libraries, with maximum complexity, is achieved with synthetic oligos with a randomized region...associated with otherwise more complicated assembly approaches, and opens the door for new opportunities in ...