We narrowed to 610 results for: cas

1. Rabies and Neuronal Tracing

   Type

   Blog Post

   Published

   May 29, 2018, 1:51 p.m.

   ...starter cells (infected with G-deleted RABV). In this case, G-deleted RABV will infect the starter cell. RABV...

2. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...dsRNA matching a specific mRNA sequence triggers a cascade of events that results in the degradation of the...

3. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

   Type

   Blog Post

   Published

   May 22, 2018, 1:38 p.m.

   ... Council of Advisors on Science and Technology [PCAST] (2012). Engage to Excel: Producing One Million ...

4. A Guide to Designing a Scientific Poster: Content, Layout, and More

   Type

   Blog Post

   Published

   Oct. 22, 2019, 12:20 p.m.

   ... a two, three, or four column style, and in all cases the idea is the same; the reader will navigate the...

5. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   Published

   June 22, 2023, 1:15 p.m.

   ...mechanisms before you start an experiment, just in case something does happen.   Finally, the best way to...

6. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   Published

   May 4, 2021, 1:15 p.m.

   ...community has created hundreds of useful variants of Cas9. By providing access to the plasmids encoding antibodies...

7. Plasmids 101: Terminators and PolyA signals

   Type

   Blog Post

   Published

   March 31, 2016, 2:30 p.m.

   ...rho-dependent and rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination...

8. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   Published

   March 15, 2016, 2:30 p.m.

   ...more accurate proviral integration assay. In either case, properly titering your viral prep is the critical...

9. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

   Type

   Blog Post

   Published

   June 5, 2014, 3:32 p.m.

   ... ourselves marketable to the wider world and occasionally thinking about how we could bypass the establishment...

10. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type

    Blog Post

    Published

    June 22, 2017, 2:30 p.m.

    ...targeted to the marker proteins, however, in this case the cell needs to be fixed and the cell membrane...

11. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    Published

    May 4, 2017, 2:30 p.m.

    ...perform in situ hybridization experiments. In many cases fluorescent proteins have usurped old methods or...

12. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ...choose from to perform these experiments. In most cases, there will be more than one right answer, so don...

13. PhD Applications After COVID

    Type

    Blog Post

    Published

    Jan. 5, 2023, 2:15 p.m.

    ...you intend to work with, so reach out to three in case one isn’t able to respond to your email. During ...

14. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    Published

    April 19, 2016, 2:30 p.m.

    ...such as TagRFP. Problem 3 can be solved, in most cases, by using monomeric FPs or FPs from different families...

15. Light Sheet Fluorescence Microscopy

    Type

    Blog Post

    Published

    July 16, 2015, 2:30 p.m.

    ...air bubbles or high concentration of fluorophores) cast shadows along the illumination path. The shadows...

16. Antibodies 101: Producing Recombinant Antibodies

    Type

    Blog Post

    Published

    Aug. 13, 2024, 1:15 p.m.

    ...directly into your preferred buffer. If that’s the case, a buffer exchange can be performed using a desalting...

17. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...antibody (HCab), which are unique to camels, llamas, alpacas and other camelids. In terms of structure, HCabs...

18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    Published

    Sept. 8, 2016, 2:30 p.m.

    ...fluorescence upon binding to its cognate protein. In some cases this property eliminates the need for removing excess...

19. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ...

20. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    Published

    May 19, 2016, 2:30 p.m.

    ...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out ...