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Showing: 541 - 560 of 793 results

  1. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...side note, my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn.... If you really got along well, suggest a coffee chat in the near future. Even just one more meeting (...

  2. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...iPSCs Symposium earlier this year, I jumped at the chance. Here was an opportunity to see Shinya Yamanaka...field have described the Yamanaka paper as "life changing". Dr. Yamanaka himself, during his keynote address...

  3. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...with the content we store in them: music, emails, chats, pictures, and apps. Throughout my time as a graduate...to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, or Z. Some...

  4. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...this into the agenda ensures that everyone gets a chance to talk and less vocal team members still have ...meeting a day in advance. This gives everyone a chance to review the agenda and add anything that is missing...

  5. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...target binding, Cas9 undergoes a conformational change that positions the nuclease domains to cleave opposite...them in your experiments. Use the following flow chart to get you thinking about the types of experiments...

  6. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...spatially limited studies and can fluctuate with changing culture conditions. The use of low intensity light...References Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl...

  7. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...questions. If you are perceived as too busy to be approachable mistakes will be made that could have been prevented...with progress reports, meeting minutes and plan changes. Written documentation helps make sure everyone...

  8. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...the current members to share short-term goals and chat about awesome science. Important factors in the ...is the lab? While larger labs tend to be more "chaotic" and offer less micromanaging, they typically offer...

  10. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...easier to navigate. These practical and effective changes will give you the ability to find the plasmid with... community of scientists) for suggesting these changes, and the SnapGene and Addgene software development...

  11. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ... cells within the dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct...microscopy (University of Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes...

  12. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...and David Root from the Broad Institute and Alex Chavez from the Wyss Institute were instrumental in helping...research and discovery, while also pushing us to challenge assumptions, holding us to nonprofit best practices...

  14. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...original publication but its name was subsequently changed to clarify that it does not contain the trademarked... information, see the manual provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This...

  15. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while this method is certainly long...the sample type and size.) It can be quite the challenge, and take a few rounds of adjustments, to get ...

  16. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting the binding region...fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering applications...

  17. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...as the LTRs or structural proteins are not interchangeable between the two systems, other, more general...j.gendis.2017.04.001 Maetzig T, Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology...

  18. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ...neurons reside.   Impact for scientists While the mechanisms by which retrograde AAV work are not yet understood...

  19. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...manipulations (CRISPR, etc.), and finish with the characterization of a function or phenotype in the surviving...significant survival advantage, such as a non-essential mechanism or any pathway with cellular redundancies, a screen...
Showing: 541 - 560 of 793 results