We narrowed to 745 results for: nes
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TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....Japan, of course, it is optimized for living for Japanese, which is not trivial, and the grant situation...eager to interact, which I don't think is good for Japanese science. I really miss the intellectual environment...
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Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog PostPublishedJuly 25, 2024, 1:15 p.m....variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available. Labs with limited...reader as opposed to chemifluorescent, and chemiluminescent methods that require specialized equipment... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m....Further Reading Quiñones-Valles, C., Espíndola-Serna, L., Martínez-Antonio, A., Quiñones-Valles, C., Espíndola-Serna... -
Tips for Writing a Good Cover Letter
TypeBlog PostPublishedMay 21, 2024, 1:30 p.m....Other fields, often highly technical or skills-based ones, may not use them very often. Similarly, entry-level... cover letter should be no more than a page. Conciseness is key! If possible, address to the professor... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....easier. You should also demonstrate a strong willingness to help others with their problems, a general...sorts of details about the application, just not the ones that matter. Therefore, if there is a bit of a Sherlock... -
An Inside Look into Addgene's Dev Team
TypeBlog PostPublishedJan. 23, 2020, 2:45 p.m.... than optimal user experience and the overall soundness of the software design. Each of us brings slightly...Demo details for this would be something along the lines of showing that the checkout takes the user directly... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m....base pairs long and found upstream of their target genes. The sequence of the promoter region controls the...elements, insulators, and silencers; however, some "leakiness" of transcription may occur. This is normally ... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m....constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate...illumination, substantial PA accumulates in the plasma membranes, indicated by the PA probe colocalizing with SuperPLDx30... -
Filming Science Videos in the Age of Social Distancing
TypeBlog PostPublishedMay 20, 2021, 1:15 p.m....rules) to some volunteers, who filmed with their phones. Test: I loved the end result, but it was clear.... Build: If you’ve ever watched any behind the scenes footage in the last several years, you can usually... -
Replacing Paper: Tips for Choosing an Electronic lab Notebook
TypeBlog PostPublishedJuly 10, 2018, 1:08 p.m....need to keep paper records as well as electronic ones? It is important to know in which ways your data...functionalities such as: electronic signatures, electronic witnessing and audit trails. How does the ELN provider ... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog PostPublishedDec. 2, 2019, 2 p.m....dissociate from the ligase and bind to exposed lysines on neighboring proteins. Thus to capture protein...monitor protein-protein interactions Split-BioID combines a protein fragment complementation (PCA) technique... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...the scientists and scientific process behind the scenes to the new antibodies and research coming out of... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m....talks for non-academic settings. Many of these guidelines will apply to any type of science presentation...supposed to be done. They will start looking at their phones. Speaking longer just makes you seem unprepared... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog PostPublishedApril 5, 2018, 12:42 p.m....a challenge. Enter Easi-CRISPR: a method that harnesses the power of ssDNA donor molecules for homology...mouse embryonic stem cells. After selecting for clones with the recombined product, you’d microinject ... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog PostPublishedNov. 6, 2015, 3:30 p.m....skills, positive disposition and motivation, open mindedness, and the ability to accept feedback and adapt...receive immense personal gratification as they witness their student’s progress and increases in self ... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog PostPublishedApril 16, 2024, 1:15 p.m....amino acid peptide contains a cluster of four cysteines (CCXXCC, where X is any non-cysteine amino acid...the fluorophore’s microenvironment to enhance brightness, stability, or other properties is an active ... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog PostPublishedMarch 7, 2023, 2:15 p.m....Statistics – The basics Statistical analysis is what determines if there is statistical significance among data...There are many, many statistical tests beyond the ones detailed in this article. These are just some of... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay for detection of extremely rare mutations... probe DNA-immobilized magnetic beads and chemiluminescence detection." Analytical Sciences 29.7 (2013... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog PostPublishedNov. 6, 2018, 1:12 p.m..... Yu, J., et al., Induced pluripotent stem cell lines derived from human somatic cells. Science, 2007....duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced ... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog PostPublishedJan. 13, 2025, 9:49 p.m....of prime editing tools. We’ll summarize the major ones in Addgene’s repository later in the post. Advantages...). Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases...