We narrowed to 628 results for: rel
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TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....enzymes Cas9 – The genome engineering workhorse You rarely hear the word ‘CRISPR’ without shortly thereafter...
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Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....going back two steps, some require starting over entirely, and our software needs to handle these cases.... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....feature you want to verify because you won’t get reliable sequence data back for the first 50-100 base pairs... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....people talk about off-target interactions. That’s relevant if you want to make sure you don’t target anywhere... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m.... to the number of strands in the canonical beta-barrel structure. Most frequently, the proteins are split... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....detect proteins in their samples. One technique that relies heavily on antibodies is ELISA, which stands for... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m....received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in over... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m.... refined and user friendly, to own the process entirely remains a large undertaking. Priority number one... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m.... was devastating. At the same time, she has an entirely new path to discover and gives her time to give... -
A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m....we use. Scientists utilize an array of tools that rely on DNA amplification and manipulation to produce... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....search and replace” genome editing technique that relies on a fusion enzyme of Cas9 nickase + reverse transcriptase... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m.... order. (It’s also why you can’t skip a module entirely, but instead will have to replace it with a spacer... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog PostPublishedSept. 26, 2019, 12:50 p.m....proteolytic site connecting the two proteins, thus releasing Cas9. Nanoblades can also deliver Cas9 variants... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....characterize the transcriptome. The first data release for CeNGEN is planned for August 15th, 2019. For... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....HDR as the double-strand break repair pathway, relying instead on microhomology-mediated end-joining (... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....be indicated in the “Species Other” column. Relevant Mutations Indicate the amino acid change as ... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....Beckman Coulter’s Biomek FXP. We had a previous relationship with Beckman Coulter and that certainly played... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m.... NYN PAMs, but with less efficiency and less reliability. Notably, the SpRY variant didn’t display higher... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m.... and minimal toxicity, including in clinically-relevant lines such as CAR-T cells. Plus, this method is... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....unlike other well-characterized Cas enzymes, which rely on multiple active sites. In addition, Casɸ has ...