We narrowed to 842 results for: Eras

...lines in tissue cultures takes half a year, and generating a transgenic plant can take even longer, a transient...are investigating how the production of cancer therapeutic drugs, vinblastine and vincristine, are regulated...transgenic hairy roots takes at least six months. Generating a transgenic plant takes even longer, is more... that neither a stable cell line or plant is regenerated from the transformed tissue. Gene function is...by working with young seedlings and optimizing several other aspects of the transient transformation procedure...Once we were on the right track, we switched to luciferases to more easily quantify the effect of the different...GUS::tMAS to confirm transformation success. Luciferases are great quantitative reporters and use a substrate...

...composed of actin and myosin which interact with each other to generate forces that cause cells and muscles...signalling pathway, which is a key regulator of all bilateral animal development. SHH plays a key role in proper...into the right and left hemisphere. Addgene has several plasmids that contain SHH, you can find them all...that are necessary for myosin activity and thus interaction with actin in nonmuscle systems in the fly. Spaghetti...other hand, is important in the formation of the visceral mesoderm which differentiates into the muscles...Embryos that lack bagpipe have severely disrupted visceral mesoderm and subsequently the gut does not form...is required for specification of the heart and visceral muscles in Drosophila." Development118.3 (1993...

...directly into at least one and preferably more than one of these five general purpose repositories. RSpace...own pros and cons. All ELNs have the virtue of liberating data from paper into an electronic environment...data out of the ELN; and (2) facilitate inter-operability with other research tools and infrastructure ...of Edinburgh, the University of Goettingen, and several other large research institutions. The following...repositories Over the past decade or so a number of general purpose repositories have been launched and are... associated with publications into one of five general purpose open source data repositories: Dataverse...one foot on the other side of the pond through interacting with colleagues in Edinburgh. You can follow ...

...what online tools people generally like to use to share ideas – with the general goal of seeing if and how...their disciplines. Included in the article is an interactive chart where you can see the yearly interdisciplinarity...abstracts identified. The journal Cell had the largest average view count per video abstract at 2,947 views. The...Gastroenterology (1,046 views) rounded out the top four average video abstract view counts.” For researchers looking...offers a low-barrier opportunity for researchers to leverage the video medium to communicate their research...their contribution. It offers a 5-step process and several examples for creating effective research videos... botanist and oceanographer who has a blog and several videos on sharing your research through video. ...

... are several factors that may prevent your gene from being up-regulated. For instance, generally, the ...known functions has the potential to be used in therapeutics, as it can increase gene expression at certain...be used to map molecular pathways and genetic interactions. Additionally, large scale CRISPR-based screens...Cas9 cutting activity, off target effects are generally not regarded as being a large problem for Cas9...driving aberrant transcription. That being said, we generally pick guides by putting the promoter of the gene...innovative technologies. To date his group has develop several popular CRISPR-based tools for altering DNA sequence..., Garnett MJ (2019) Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens. Nature 568...

...them remain uncharacterized. This represents a considerable barrier to furthering research and developing...Cancer Center tackled this problem by developing a moderate throughput functional genomic platform to annotate...over 1,000 mutations, and the group has deposited several hundred plasmids from this study with Addgene. ...research team identified 1,049 perturbations in several genes spanning 33 cancer types. The types of mutations...indels (Ng et al., 2018). From this data, they generated lentiviral constructs using the High-Throughput...cell lines and any mutations that that confer proliferation advantages will be revealed in the absence of...is important for the development of targeted therapeutic interventions for cancer. This screening platform...

... visualize their activity. In 2019, the first generation of norepinephrine sensors was developed, named...norepinephrine (Feng et al., 2019). But this first generation of tools had limited sensitivity and speed and... models of nLightG (left) and nLightR (right) generated using ColabFold (Mirdita et al., 2022). B, Representative...Speed One of the major advantages of this next generation of norepinephrine sensors is their speed. nLightG...GRABNE were more sensitive than nLightR, both generating a measurable signal after electrical stimulation...test point, ten pulses. This is in line with the general findings that red fluorescent proteins tend to ...improvements. In particular, they’re focused on generating variants with improved affinity and dynamic range...

... gene expression in yeast Megan McClean’s lab generated an optogenetic toolkit that controls repression...frequency of genome-wide off-target DNA editing. Using several rapid and cost-effective screening methods, David...with reduced Cas9-independent deamination. They generated these editors using the BE4max architecture. The...reported in this study have ~10-100-fold lower average Cas9-independent off-target DNA editing, ~5–50-...off-target DNA editing, and maintain ~50–90% of average on-target DNA-editing levels relative to BE4max...division, and cellular differentiation, but it takes several weeks to delete or deplete individual genes. Michael...depending on transgene design and delivery. They generated hPSC lines with AAVS1-integrated, inducible, and...

...such a modular platform could accelerate the development of genetic therapies even further. In the meantime...successfully treated with a CRISPR gene editing therapy personalized to his genetic mutation. “Baby KJ”...hospitals, universities, private companies, and federal regulators. But the speed is impressive on a larger... ABE was about 50% efficient in human cells on average, but its effectiveness varied depending on the ...coincidence, KJ’s genetic sequence was able to tolerate bystander edits—unintended changes to nearby target... Lam, D. K., Rees, H. A., Solá-Esteves, N. M., Barrera, L. A., Born, D. A., Edwards, A., Gehrke, J. M....adenine base editors with increased activity and therapeutic application. Nature Biotechnology, 38(7), 892...

...process, I’ve provided guidance and mentoring to several people that found my insights helpful. All went...of candidates either get no interviews, or get several interviews but no job offers, is a failure to clearly...understand your proposal to give you proper consideration. 3. Present an easy to follow seminar See item...techniques. You DO need to be prepared to know the literature of your field and all of the caveats and alternatives...human health. Failure to do so is implicated in several human diseases. Proper patterning of cells and ...that we can manipulate to study pathology and therapies." This is only the first few minutes of the...incorporated example documents. This part has generated a lot of positive feedback. Many of these documents...