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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 836 results for: Eras

Showing: 561 - 580 of 836 results

  1. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ...organizations to share materials more broadly to accelerate COVID-19 research: Sinai Health System                                     ... available to industry? Over the past 8 years, several organizations have made their plasmids available...Shop’ for Fluorescent Proteins, and plasmids from several labs at Florida State University became openly ...

  2. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...back from other summer programs. Amy took into consideration just how busy I was and ensured that my decision...and the insert size should be a max of 500 bp (preferably). I compiled a list of three options from the...Through Addgene, I learned the basics of lab safety, general work skills — such as using Google workspace — ...

  3. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...needs to integrate within an exon of a gene to generate a transcriptional fusion. This may disrupt the...reading frame, in fact, they can be activated a considerable distance away from a gene which can make identification...there are many ways to insert your construct to generate a collection of monitored genes and mutants - ...

  4. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ...seqWell and going into detail about how we use next generation sequencing results to perform quality control... We’ve also talked about how our new Snapgene generated maps provide improved feature detection with an...is always to send prepped plasmid DNA for next generation sequencing. We then copy the contents of the ...

  5. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...developed by Thomas Clandinin’s lab, and incorporates several common genetic tools in Drosophila (Fig. 1):  ...Ribozyme), a self-cleaving ribozyme. In the second-generation SPARC2 system, HDVR is placed upstream of the...organisms including mouse and fish, SPARC is likely generalizable to other systems as well. References: del...

  7. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...associated with the brain’s reward circuits are generally linked to dopamine (DA), a well-known reward chemical...more mysterious. Thus, scientists have developed several ways to sense dopamine. Methods for measuring Dopamine...Pavlovian conditioning Find the GRABDA sensor here! Overall these sensors are powerful new developments in ...

  8. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...familiar with the alarming amount of waste that is generated. This is a systemic problem that we need to address...account for an estimated 2% of global plastic generation. Less than 9% of plastics are ever recycled, ...boxes). We get these boxes a lot when receiving temperature-controlled reagents. Expanded polystyrene will...

  9. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...EF1ɑ-driven viral vectors (AAV1) - and you can also find several other constructs in plasmid form. Fig. 1...neuroscience-related proteins, but we recently added several broadly useful antibodies for detecting protein... of the human proto-oncogene c-Myc. Since the generation of the first anti-c-Myc [9E10] (Even, et al.,...

  11. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...inefficient process– just 1 out of 10,000 cells on average! Without some means of quickly determining which...resistance!  What are antibiotics? Antibiotics are generally defined as agents that kill bacteria, or inhibit...lab, their mechanism for killing bacteria, and general working concentrations. For instructions on how...

  12. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...biological tools, and with the right safety considerations, they aren't dangerous for controlled lab usage...and episomal expression will last ~2-7 days at moderate levels. With regard to target cells or organism...Transduce on! Resources Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling...

  13. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...unfold, absorbing the stochasticity of molecular interactions and embedding individual diversity to get from...than what fits for applied research. I focus in general terms on a couple of very broad topic domains, ...networks, as much as with technical knowledge. I leverage a breadth of knowledge to put diverse aspects ...

  14. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...find some proteins of interest for which you have several antibodies in stock. Why are there so many options...let’s think about induced conformation changes. Several types of assays, such as ELISAs and Westerns, frequently...complex if you’re looking for proteins that are interacting with one another, or if you’re looking for a ...

  15. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...highlights from the past couple months including several pooled libraries: Cas13X and Cas13Y, two compact...It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied...the new viral preps from recent months: Second generation GRAB dopamine sensors from Yulong Li’s lab are...

  18. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...translate to a desire to do the research itself. Several years of exclusively writing lab reports and empirical...busy point of the first semester in which I had several projects nearing deadline (an animated video depicting...chance to carry out a comprehensive review of the literature informing the notion of “heading” in soccer as...

  19. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...testing at Addgene meaning they come ready made to accelerate your research.   Why distribute virus? Plasmid...This service will further enable scientists and accelerate research." What viral preps are available...expression of a gene under the control of the tet operator. AAV AAVs are the workhorses of both chemo- and...

  20. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ... due to a few limiting factors.  To start, the average health science startup requires a great deal more...those ideas into commercial products and next generation companies.  We launched in the early spring of...(CFO).  As CEO, I am responsible for internal operations and building external strategic partnerships ...
Showing: 561 - 580 of 836 results