...EF1ɑ-driven viral vectors (AAV1) - and you can also find several other constructs in plasmid form. Fig. 1...neuroscience-related proteins, but we recently added several broadly useful antibodies for detecting protein... of the human proto-oncogene c-Myc. Since the generation of the first anti-c-Myc [9E10] (Even, et al.,...

...Addgene is a non-profit, we can focus more on generating blog content that scientists will find useful...giving it a high five. While screaming can be therapeutic and I’ve certainly done my fair share of venting...management, information distillation, etc) are transferable to many different fields. If you start thinking...

...inefficient process– just 1 out of 10,000 cells on average! Without some means of quickly determining which...resistance!  What are antibiotics? Antibiotics are generally defined as agents that kill bacteria, or inhibit...lab, their mechanism for killing bacteria, and general working concentrations. For instructions on how...

...testing at Addgene meaning they come ready made to accelerate your research.   Why distribute virus? Plasmid...This service will further enable scientists and accelerate research." What viral preps are available...expression of a gene under the control of the tet operator. AAV AAVs are the workhorses of both chemo- and...

...fluorescent dyes for DNA-protein interaction studies? Kyubong Jo's lab generated fluorescent protein fusions...lentiviral vectors the iBARs can be detected by next generation sequencing.  In order to avoid a high false-positive...

...translate to a desire to do the research itself. Several years of exclusively writing lab reports and empirical...busy point of the first semester in which I had several projects nearing deadline (an animated video depicting...chance to carry out a comprehensive review of the literature informing the notion of “heading” in soccer as...

...find some proteins of interest for which you have several antibodies in stock. Why are there so many options...let’s think about induced conformation changes. Several types of assays, such as ELISAs and Westerns, frequently...complex if you’re looking for proteins that are interacting with one another, or if you’re looking for a ...

...highlights from the past couple months including several pooled libraries: Cas13X and Cas13Y, two compact...It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied...the new viral preps from recent months: Second generation GRAB dopamine sensors from Yulong Li’s lab are...

...screening, and next-generation sequencing of hybridomas have opened the door for a new generation of recombinant...Animal-based methods for antibody production have several drawbacks. Polyclonal antibodies produced in animals...

...biological tools, and with the right safety considerations, they aren't dangerous for controlled lab usage...and episomal expression will last ~2-7 days at moderate levels. With regard to target cells or organism...Transduce on! Resources Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling...

...unfold, absorbing the stochasticity of molecular interactions and embedding individual diversity to get from...than what fits for applied research. I focus in general terms on a couple of very broad topic domains, ...networks, as much as with technical knowledge. I leverage a breadth of knowledge to put diverse aspects ...

... due to a few limiting factors.  To start, the average health science startup requires a great deal more...those ideas into commercial products and next generation companies.  We launched in the early spring of...(CFO).  As CEO, I am responsible for internal operations and building external strategic partnerships ...

...enter cultured cells and activate expression of a luciferase reporter. CPP was not required for protein entry...cells! After verifying the specificity of their luciferase assay, they concluded that components in the ...lethality and were sorted into single wells to generate clonal populations. After iTOP treatment, both...

...However, careers outside of the lab highlight “transferable” skills such as leadership, management, and ...impact your work? What design variables need consideration? Enrich your knowledge and skills outside of...tools such as the Individual Development Plan to generate a list of potential professions Google potential...

...example, the hedgehog gene can be overexpressed in several cell types by using different Gal4 drivers. It ...explain the relatively straightforward process of generating your own fly lines. In the meantime, be sure ...expression as a means of altering cell fates and generating dominant phenotypes., Development 118, 401–15...

...-rings are, in fact, permeable to CO2 at low temperatures (-80°C) and once in the tube, the CO2 can alter... storage, we then thawed the samples at room temperature and used the color of the liquid solution and...not all O-rings) were permeable to CO2 at low temperatures. We tested two different types of tubes: the...

..., why it affects our emotions, and even what therapeutic properties it has. As with other works of Dr....of characters from five different families over several millennia as their paths cross with each other ...Nicholson Baker’s The Anthologist. The book ended up literally changing my life. Though I have an undergraduate...

...reading, you can calculate the DNA concentration. Generally, A260 of 1.0 is equivalent to 50 ug/ml pure dsDNA...samples and are often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, .... This method is often used preceding next-generation sequencing or microarray studies and is not used...

...in a cell or animal model. AAV plasmids contain several important elements, but among the most important...occurring.  How do I validate my ITR sequences?  Next-generation sequencing and Sanger sequencing might fail to...vector stability and sequencing results. Next-generation sequencing is also helpful in quantifying ITR...

... need a second GFP antibody?  Similarities:  Generated against full length GFP and converted to recombinant... also withstands denaturing conditions, high temperatures, and other chaotropic conditions better than...fluorescence in high molarity GdnHCl, at high temperatures, and in the presence of oxidizing agents. Adapted...