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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 791 results for: Kin

Showing: 561 - 580 of 791 results

  1. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ... blue, indicating that your IPTG and x-gal are working as they should be. Don't rush the process: It...between blue and white colonies. Take care in making your plates: X-gal is light and temperature sensitive...

  2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22...

  3. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...of the same technical advantages that come with working with prokaryotes. This includes but is not limited...selection rather than antibiotic selection when working with yeast, due to high rates of spontaneously ...

  4. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ... cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease...about the history. How had investigators done this kind of work before? Musunuru: In the "olden days," not...

  6. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...people knew how to access Addgene, and that we were making it possible for scientists to both deposit and ...through physical outreach, visiting organizations, knocking on doors, and attending conferences. And when ...

  7. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...proteins in your assay.  Narrowing the field After working your way through the above steps, you may find ...an antibody website. In 2016, the International Working Group for Antibody Validation proposed 5 pillars...

  8. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...developed by engineering the Cas12f scaffold gRNA and making strategic mutations in the DNA binding cavity of...half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging. Most importantly...

  10. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...unpublished results at a conference? Post your working manuscript as a preprint too and you can share ...American Society for Cell Biology in a position working with preprints in life sciences. He also posted...

  18. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ... to own the process entirely remains a large undertaking. Priority number one was to ensure that this ... load it on to our new MiSeq and set up the run making sure to note the appropriate barcode for each sample...

  19. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... ability to study and understand the complex interworking of the 86 billion neurons in the human brain... opens the door to in vivo experiments such as tracking the synaptic maturation pattern of hippocampal...

  20. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...detection We and other members of the lab had been working on advancing the paper-based platform toward a ...when we needed additional expertise. Perhaps most striking is that there were seven institutions involved...
Showing: 561 - 580 of 791 results