We narrowed to 610 results for: REV

1. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   Published

   Sept. 28, 2017, 2:01 p.m.

   ...restricted transgene expression in the CNS and PNS Previously to broadly introduce a transgene into a specific...

2. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   Published

   Dec. 7, 2020, 2:53 p.m.

   ...within mixed communities and into species not previously cultured or isolated (Rubin et al., 2020).  ...

3. Plasmids 101: Colony PCR

   Type

   Blog Post

   Published

   May 12, 2016, 2:30 p.m.

   ...generalized expected results for the three primers previously described. When using insert-specific primers...

4. Tips from the Repository Trenches: Using Barcodes to Track Samples

   Type

   Blog Post

   Published

   Oct. 6, 2015, 2:30 p.m.

   ...people or even multiple labs. Careful tracking prevents us Addgenies from wasting hours of valuable time...

5. The Importance of a Fun Workplace: Company Culture at Addgene

   Type

   Blog Post

   Published

   Sept. 3, 2015, 2:30 p.m.

   ...join together to feast and play lawn games. In previous years, this event was held at Executive Director...

6. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

   Type

   Blog Post

   Published

   Dec. 2, 2014, 7:23 p.m.

   ... duty for researchers when considering the peer-review system of journals and grant applications, for ...

7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   Published

   May 15, 2014, 3:33 p.m.

   ...strategies such as FRET, calmodulin, and others. Review Addgene's collection of fluorescent biosensors ...

8. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   Published

   July 30, 2024, 1:15 p.m.

   ...deeply interested in pursuing them. We’ve recently revamped our viral vector selection process to standardize...

9. Antibodies 101: The Four ELISAs and When to Use Them

   Type

   Blog Post

   Published

   July 25, 2024, 1:15 p.m.

   ...set up. Then they block the capture reagent to prevent non-specific interactions. Next comes the binding...

10. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    Published

    May 21, 2024, 1:30 p.m.

    ... applying to. Be clean and professional. Always review grammar and spelling several times and ask someone...

11. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    Published

    April 11, 2024, 1:15 p.m.

    ...Alcaligenes eutrophus, Azotobacter vinelandii, Brevibacterium methylicum, Caulobacter crescentus, Desulfovibrio...

12. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ...phosphatases that may degrade the target protein. To prevent this, include protease and phosphatase inhibitors...

13. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type

    Blog Post

    Published

    July 7, 2020, 1:15 p.m.

    ...circumvent sequencing and the need for a DSB in their previous reporter, the Harris Lab created a panel of eGFP...

14. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ... RPA (recombinase polymerase amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled...

15. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ...Dr. Amanda L. Gunn  and Dr. Denise M. Akob for reviewing and commenting. Binning Singletons is especially...

16. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    Published

    April 11, 2017, 2:30 p.m.

    ...In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies.” Chem Biol...

17. Lab to Office Culture Shock

    Type

    Blog Post

    Published

    Feb. 5, 2019, 1:04 p.m.

    ...skills are the non-technical skills you pick up wherever you work, and you’ve almost certainly developed...

18. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ...groups to bases at specific nucleotide sequences, preventing the cognate restriction enzyme from cleaving ...

19. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ... used across species, for example, in both S. cerevisiae and E. coli. Auxotrophic selection is especially...

20. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ...rapidly iterate and optimize our pipeline. After reviewing and testing several platforms for engineering ...