We narrowed to 610 results for: cas

1. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   Published

   Sept. 28, 2017, 2:01 p.m.

   ... infect the cell type of interest, which, in the case of Chan et al, was neurons and astrocytes. Why ...

2. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ...fluorescence upon binding to its cognate protein. In some cases this property eliminates the need for removing excess...

3. Tips for arabidopsis transformation

   Type

   Blog Post

   Published

   Oct. 25, 2018, 1:23 p.m.

   ... blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering...

4. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   Blog Post

   Published

   Aug. 9, 2016, 2:30 p.m.

   ...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ...

5. Lentiviral Vector Uses and Overview

   Type

   Blog Post

   Published

   May 19, 2016, 2:30 p.m.

   ...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out ...

6. Plasmids 101: Protein tags

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   Blog Post

   Published

   Dec. 11, 2014, 4:26 p.m.

   ... blots, IP, co-IP, IF, flow -cytometry; can occassionally interfere with protein folding HBH HHHHHHAGKA...

7. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

   Type

   Blog Post

   Published

   Dec. 2, 2014, 7:23 p.m.

   ... series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system enables...

8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   Published

   May 15, 2014, 3:33 p.m.

   ...used to study protein-protein interactions. In this case, two portions of EGFP are fused to the proteins ...

9. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...per 100 ml of starting blood sample, in the best-case scenario [7]. In the clinical setting where patient...

10. Transferable Skills Guide: Career Planning Resources

    Type

    Blog Post

    Published

    Nov. 14, 2017, 2 p.m.

    ...outside of the box (most likely your lab, in this case). It is important to realize that you have to take...

11. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post

    Published

    Sept. 17, 2019, 12:40 p.m.

    ...harbouring a Cre-dependent IL-1 beta overexpression cassette. Expression of Cre in these neurons turns on overexpression...

12. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ...both homeobox genes that are part of a genetic cascade that determines the cell fate of the dorsal mesoderm...

13. Lab to Office Culture Shock

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    Blog Post

    Published

    Feb. 5, 2019, 1:04 p.m.

    ...inevitably have downtime and plenty of it. That’s not the case at an office job. I’ve frequently told job candidates...

14. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ...Nextflow for bioinformatics pipelining In many use-cases, including ours at Addgene, the overall purpose ...

15. How to Write a Scientific Review Article

    Type

    Blog Post

    Published

    Feb. 16, 2017, 3:30 p.m.

    ...Finally, you can sound credible and cool when you casually mention “Haery et al., showed that MYC expression...

16. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    Published

    Jan. 12, 2017, 3:30 p.m.

    ...product or plasmid with an attP donor vector. In this case, we would use PCR to add attB sites to either end...

17. Antibiotic Resistance: An Old Solution but a New Problem

    Type

    Blog Post

    Published

    Nov. 10, 2016, 3:30 p.m.

    ...even make their own antibiotic compounds. In such a case, the microbe itself would be unharmed by the compound...

18. Antibodies 101: Reading a Flow Plot

    Type

    Blog Post

    Published

    Feb. 6, 2024, 3:13 p.m.

    ... by looking at the different populations. In the case of Figure 2, one population is B- and another two...

19. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ...edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies...

20. Viral Production at Addgene

    Type

    Blog Post

    Published

    Oct. 24, 2023, 1:15 p.m.

    ... contamination. To date, Addgene has never had a case of mycoplasma contamination. In the event of contamination...