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TypeBlog PostPublishedFeb. 1, 2018, 3:07 p.m.... identify the same feature in other plasmids. Aligning sequences with Snapgene Once we’ve identified...plasmid contains a region with a high amount of guanine and cytosine nucleotides, we might need to send...
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Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics
TypeBlog PostPublishedJune 11, 2019, 1:16 p.m....There’s a steep learning curve for Inkscape, but there are lots of resources for learning the software. ... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog PostPublishedDec. 14, 2020, 2:15 p.m....showing the effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists...plasmids are available as a collection, facilitating screening across the transducerome, to gain a more complete... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the cap gene which differ...334 . https://doi.org/10.1007/s40259-017-0234-5 Büning H, Srivastava A (2019) Capsid Modifications for... -
Management for Scientists: Giving Feedback
TypeBlog PostPublishedFeb. 6, 2015, 2:46 p.m....bring about change in behaviors and habits in a nonthreatening way. However, I have also found that scientists...interpretation of my feedback to you. What are We Learning: What would have happened if _________? What ... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog PostPublishedApril 15, 2014, 3:11 p.m.... precious in less resourced areas of the world. Nina Dudnik, Founder of Seeding Labs, tells the story...bothered by the fact that I was wasting buffer running my tiny gels in a normal gel box. It took a bit... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody...well. References Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, ... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m.... controlled with light without the need of any cloning. What advantages does BLADE have over AraC...lawn on an agar plate, put a mask over the plate containing the pattern of the image, and shined blue light... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m....Addgene prides itself on being financially self-sustaining through distributions of research materials, ... advances in gene synthesis, affinity reagent screening, and next-generation sequencing of hybridomas ... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....undergo rigorous quality control testing at Addgene meaning they come ready made to accelerate your research...both chemo- and optogenetics. We’re therefore beginning our AAV viral services with these tools. A variety... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m.... which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen. This fluorescent...suited for certain studies. If you have plasmids containing mNeonGreen, mTFP1, or mWasabi that you would ... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog PostPublishedJan. 24, 2019, 2:24 p.m....Figure 2). We’ve also created constructs with containing chemical tags such as the SNAP-tag, Halo-tag,...17972876. Kohl, Johannes, et al. "Ultrafast tissue staining with chemical tags." Proceedings of the National... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....time to make virus! The constructed viral vector containing the cargo of interest is then typically transfected...transduced (like a rogue HEK-293 cell). This virus-containing media can then be placed on whatever cells you... -
Plasmids 101: Origin of Replication
TypeBlog PostPublishedNov. 11, 2020, 2:23 p.m....CDF) D Relaxed This table defines common cloning vectors, their copy number, ori, and incompatibility...are especially useful to keep in mind if you are planning to purify your plasmid DNA: The insert Bacteria... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....hot springs were known to be diversity hotspots containing abundant biological dark matter and so the lab...leave your samples behind! Paul and Anastasia (Potanina) Neddersen characterized a few samples by 16S ... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....] is pretty great for us,” Wang said. “We were planning on and looking at a bunch of different strategies...mutations in the gene may prevent neurons from returning to basal conditions normally after periods of ... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m.... like to share my own experiences founding and running miLab to describe some key lessons I learned while... upon these people for guidance. Before even beginning the process of launching a company, you need to... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....stepwise protocol on designing and using RNA scaffolds: Delebecque, C. J. et al. Designing and using RNA scaffolds... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog PostPublishedMay 6, 2014, 1:07 p.m....calcium and other small molecules since the very beginnings of the field. Those engineered sensors were designed... began by isolating a calcium-binding protein, troponin C (TnC), from muscle cells. They identified a ... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog PostPublishedJuly 21, 2020, 1:15 p.m....DNA this way. First, start by pouring your gel containing a DNA intercalating dye (ex: ethidium bromide...research Browse protocols for molecular biology, cloning, and more ...