We narrowed to 695 results for: prot

... BCA assay uses a serial dilution of a control protein, incubated alongside an unknown sample with the... time, etc., which can vary even when the same protocol is being followed.  Finally, standard curves must...

... has 10 gRNAs per gene and targets all ~20,500 protein-coding genes. Unique, non-overlapping sites in ...: 1) targeting guides (blue), which target all protein-coding genes present in the genome; 2) non-targeting...

...interacting with proteins such as transcription factors and chromatin modifiers. These proteins recognize and...

...or other plasmid part? Do you want to fuse your protein to any tags or fluorescent markers? If you’re creating... streamline your plasmid extractions. Use this protocol shared by Dr. Rob Hurt for high-throughput minipreps...

...for multicopy chromosomal integration (ex: for protein expression) (Zhang et al., 2020). Multicopy chromosomal...pQCascade expresses the transposase TniQ, the Cas proteins Cas6, Cas7, Cas8, and the crRNA. The spacers of...

...inhibiting translation of replication machinery proteins, or directly inhibit replication by binding to... the plasmid, a centromere-like region binding protein (the CBP), and a partition NTPase (Schumacher, ...

...Resources at Addgene.org Check out our Protocol Pages for Video Protocols ...

... editing in stem cells using CORRECT." Nature protocols 12.2 (2017): 329-354. PubMed PMID: 28102837. Additional...How to Design Your gRNA Delivering CRISPR Ribonucleoproteins Resources on Addgene.org Browse All CRISPR...

...present that vary in cell type, size, and marker proteins. But even if you are analyzing a homogenous cell... only enter dead cells, while living cells can protect themselves from it. So here, I actually want to...

...   Serotypes Serotype refers to the different protein compositions of AAV capsids, which determine how... such as synapsin, glial fibrillary associated protein (GFAP), or tyrosine-hydroxylase (TH), can limit...

...amount of information regarding which regulatory proteins, signaling molecules, mutations, and environmental...combination of red, blue, and green fluorescent proteins in each state. The three methods of state interrogation...

... Require Ex Vivo Handling of Embryos. Current Protocols in Human Genetics 88: . https://doi.org/10.1002... CRISPR‐Cas9‐Mediated Genome Editing. Current Protocols in Mouse Biology 6:39–66 . https://doi.org/10.1002...

...light can damage DNA and that proper personal protective equipment should be worn when visualizing using...for more detail. Find columnless purification protocols on our website. 6. Verify the plasmid After purifying...

...complexes to study neural function. Often the reporter proteins associated with these complexes are so robustly...vectors: packaging capacity and the role of rep proteins in vector purity." Human gene therapy 18.2 (2007...

...individual colonies growing out in the 96-well dish. My protocol with many additional tips can be found here. ...knockouts and knockins in human somatic cells. Nature Protocols 2007; 2(11): 2734-46. PubMed PMID: 18007609. Addgene...

...catecholamine neurons or glial fibrillary acidic protein (GFAP) for astrocytes it is important to test it...have low levels of TH mRNA without expressing TH protein indicating that there is promoter activity. Additionally...

... efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed for ...single round of editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo...

...YouTube channel for more helpful animations, useful protocol videos, and quick tips for lab success.  ...

...the Lab" shows how antibodies are used to study proteins via ELISAs, western blots, immunoprecipitation...

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