... NLuc fused to enhanced green fluorescent protein (GpNluc) and the other consists of NLuc fused to the...produce a series of FP-RLuc8 fusions for multicolor imaging. In all of these fusions coelenterazine intiates...plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions of the Enhanced... Many of our technology platforms like HaloTag® Fusion Protein, codon-optimized Firefly luciferase genes...better biosensors. NanoLuc®-fluorescent protein fusions for imaging Fluorescent proteins are fantastic ...the more penetrative orange fluorescent protein fusion (OgNLuc). Both fluorescent proteins can be excited... by Nluc emission and the lab has dubbed these fusions LumiFluor Reporters. The LumiFluor reporter constructs...

... disrupted when GFP is fused to these sequences. When N- and C-terminal fusions are not tolerated the ...heterotrimeric G-alpha subunit fusions that do not tolerate N- and C-terminal fusions (Adjobo-Hermans, 2011)....microscope, and finally (iv) good toleration in fusion proteins. Since GFP is such a well-validated, all-round...interfere with the activity or the localization of the fusion protein. Also, in cases where the protein of interest...interest is relatively small, adding GFP may alter diffusion rates. Smaller genetically encoded probes are .... GFP can only be used for tagging proteins GFP fusion proteins are made by connecting its DNA code to...are defined by a gRNA. Since Cas9 is a protein, a fusion with GFP allows for detection of genomic locations...

...interest fused to the C-terminus of SCFP3A Amber N1 Yellow Mammalian Express a gene of interest fused to ...interest fused to the C-terminus of Amber mVenus N1 Yellow Mammalian Express a gene of interest fused to ...can be used to create your choice of fluorescent fusion protein with your gene of interest: Plasmid ...mCerulean N1 Cyan Mammalian Express a gene of interest fused to the N-terminus of monomeric Cerulean mCerulean...mCerulean C1 Cyan Mammalian Express a gene of interest fused to the C-terminus of monomeric Cerulean mTurquoise2... C1 Yellow Mammalian Express a gene of interest fused to the C-terminus of monomeric Venus pCEP4YPet-MAMM...SYFP2 Yellow Mammalian Express a gene of interest fused to the C-terminus of SYFP2 Clover Green Mammalian...

...A&C-BEmax   Fused cytosine and adenine base editors Grünewald et al., 2020 SPACE   Fused cytosine and... A Cas protein (catalytically dead or nickase) fused to a deaminase that makes the edit A gRNA targeting...the inactive dCas9 (Komor et al., 2016). These fusions convert cytosine to uracil without cutting DNA....converted to thymine through DNA replication or repair. Fusing an inhibitor of uracil DNA glycosylase (UGI) to...editor using a cytidine deaminase from sea lamprey fused to Cas9 nickase (Nishida et al., 2016). Target-AID...minimum efficiency of ~50% and confirmed that the fusion protein retained the PAM properties of the individual...further decrease indel formation 1.5-2 fold, the team fused bacteriophage protein Gam to the N-terminus of BE4...

...orientation of the BirA* fusion matters! Schopp et al saw different levels of fusion protein expression and...BirA, an E. coli biotin ligase. In BioID, BirA* is fused to a bait protein and it biotinylates proteins that...Split-BioID method, each BirA* fragment is then fused to one of two bait proteins that are known to interact...proteins are labeled with biotin. For labeling, BirA* fusion proteins are expressed in cells via plasmids or...C-terminal BiraA* (C-BirA*). These two fragments are fused to two proteins, here proteins Y and Z, that are..., and that result in proper localization of the fusion protein. Advantages of Split-BioID Split-BioID’... remains even if the interaction with the BirA* fused bait proteins ceases. Lastly, Split-BioID and BioID...

... proteins fused to CGBEs Instead of using UNG, the Wei Leong Chew Lab developed two fusions of a CBE with...single-stranded DNA (ssDNA) region, using enzymes fused to Cas9 to alter the exposed ssDNA bases. Thus, ...editors target this “non-target strand.” To avoid confusion, we usually use different terminology when discussing...ABEs, the editor they were using consisted of Cas9 fused to an engineered adenosine deaminase capable of ...polymerase β (PB) (Chen et al., 2021). Both of these fusion constructs improved editing in various cell types...3838/BE_SMART/. Even more new DNA repair proteins fused to CGBEs The David Liu lab also experimented with...smegmatis, termed UdgX, they found three proteins whose fusion improved the CGBE: DNA polymerase D2 (POLD2), exonuclease...

...are described in more detail below: Fusion protein construction Fusing FP fragments to your PIPs requires...determine where to fuse the FP fragments. In the absence of this information, you can fuse each FP fragment...constructing fusion proteins, check out our article: Fluorescent Proteins 101: GFP Fusion Proteins - Making...hard-to-achieve factors. For example, FRET requires that fusion proteins be in close proximity and present in large...fluorescence. When complementary FP fragments are fused to two hypothesized protein interaction partners...more difficult with FRET, as the overexpression of fusion proteins it requires can lead to aberrant subcellular...BiFC experiment generally involves three steps: fusion protein construction, cellular expression, and ...

...Fluorescent protein fusions One of the easiest ways to track a given protein is to fuse it to a fluorescent...Instead of directly fusing a fluorescent protein to your protein of interest, you instead fuse it to the synthetic...any case you would use a traditional FP fusion, a SunTag fusion could be used as well. Pros of SunTag ...system is far from perfect. For example, If your fusion protein is expressed at low levels, you have to...copies of the short epitope GCN4. GCN4 recruits GFP fused to the cognate scFV antibody, which is expressed... 24 kDa for GFP alone. Although traditional FP fusions can also affect protein activity, half-life, or...

...dCas9-KRAB fusion, both targeted to the promoter region. Cas9 activators include Cas9 fused to VP64, VP64...Cas9 for your knockout experiment Generating a no-fuss genetic knockout  Knock-out cells or animals are... editors are dead Cas9 (dCas9) or nCas9 enzymes fused to either cytosine or adenosine deaminases to facilitate...various cargo to a specific DNA locus by directly fusing the cargo to dCas9.  This technique has been exploited...transcriptional repressor domain which is frequently fused to Cas9 for this purpose. dCas9-KRAB plasmids for...simplest of the dCas9 activators consists of dCas9 fused to a single transcriptional activation domain - ... an epitope tagged dCas9 and antibody-activator fusion proteins. The Synergistic Activation Mediator complex...

... same protein of interest tagged with different fusion proteins for different experiments over the years...idea. What if there were a way to just make one fusion protein that switches to any color or tag you want...molecule you choose (Figure 1). Just express the fusion protein and add a specific ligand! A self-labeling...structure is the protein of interest, and black is the fusion tag or labeling system. Image adapted from Lavis...2003, Kai Johnsson’s lab developed a self-labeling fusion tag based on the human DNA repair protein AGT that... Sheetz labs demonstrated in 2005 that proteins fused with E. coli dihydrofolate reductase (eDHFR) could...the tip. 649-mDia1 and 549-CP are both SNAP-tag fusions, purified and labeled separately with different...