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We narrowed to 320 results for: bre

Showing: 61 - 90 of 320 results

  1. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... is usually accomplished by Cas9-mediated dsDNA breaks: following a cut, the error-prone nature of non-homologous...although this is rarely done). When targeting a dsDNA break for HDR, the choice of target site is far more constrained...which makes DNA changes in the absence of dsDNA breaks (Rees et al., 2018). For C>T and A>G base editors...

  2. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...marker like the Hoechst stain for the nuclei. (See Brewer et al, 2022 for a sample protocol.)  Simultaneous... Nguyen, K., Van Der List, D., Murray, K. D., Engebrecht, J., & Trimmer, J. S. (2019). A toolbox of IgG... 8, e43322. https://doi.org/10.7554/eLife.43322 Brewer, M., McDonough, L., Zhu, Y., Neumann, E., De Caestecker...

  3. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...you obtained the desired chimeric mice, you would breed them many times, hoping to produce a mouse with ...need to remove the selection marker, likely by breeding to a mouse line with a recombinase. If it turned... insert two loxP sites. Since two double-strand breaks are made, it is common to see deletions of the ...

  4. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...homology arms of 35-80 bp, and DNA double-strand breaks up to 54 bp from the desired insertion site result...knock-in efficiency, presumably by channeling DNA breaks into the homologous recombination repair pathway...interesting to note that recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle...

  5. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...development (Grimm & Lavis, 2022). With the wide breadth of fluorescent tools that have been developed throughout...is an important consideration for assay design (Breen et al., 2016). When selecting dyes, it's important...(1). https://doi.org/10.1038/s41598-020-78827-9 Breen, C. J., Raverdeau, M., & Voorheis, H. P. (2016)....

  6. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...protein, first block the Golgi apparatus with Brefeldin A to inhibit secretion and then follow the typical...NK) cells.   Tips for flow cytometry success Break up cell clumps Success in a flow cytometry experiment...the sample through a nylon mesh cell strainer to break up clumps before running on the instrument. To sustain...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...al. 2022. Image created with BioRender.com. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory.../ENEURO.0373-21.2022 Brown, D., Altermatt, M., Dobreva, T., Chen, S., Wang, A., Thomson, M., & Gradinaru...Kumar, S. R., Miles, T. F., Chen, X., Brown, D., Dobreva, T., Huang, Q., Ding, X., Luo, Y., Einarsson, P... Kumar, S., Miles, T. F., Chen, X., Brown, D., Dobreva, T., Huang, Q., Ding, X., Luo, Y., Einarsson, P...

  8. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...other organisms, CRISPR-induced double stranded breaks are lethal because the non-homologous end-joining...ineffective in bacteria, CRISPR-induced double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini...genomic locations, and introduces double strand breaks, the bacteria are unable to resolve them, and the...

  9. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...HDR), the pathway for resolving DNA double-strand breaks (DSBs) preferred by researchers. Compared to non-homologous...Base editors chemically modify DNA bases without breaking the DNA backbone, sidestepping the problem of ...converts a C to U without producing a double strand break. UGI = uracil DNA glycosylase. Created with BioRender.com...

  10. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...talk to scientists from around the world making breakthrough discoveries!” I knew right then I had found .... Summaries of the latest technologies and breakthrough research. Insights about how to navigate through...

  11. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    Published
    May 24, 2016, 2:30 p.m.
    ...Horticulutre Biotechnology, he did his PhD in Plant Breeding, Genetics, and Biotechnology at Michigan State...collaboration across the globe. Listen here! Podcast Breakdown (Time: Topic) 0:00-1:00 : Intro 1:01-3:48 : Kingdom...

  13. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...We’re breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward ...plasmids through this podcast segment. Below, we break down each of the Hot Plasmids segments into its ...

  15. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ... five strains of Ebola representing the latest outbreak and earlier ones, along with links to the relevant...content. -And the latest updates on the ongoing outbreak from the CDC and WHO. - For anyone interested ...

  16. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...most CRISPR systems and generates double strange breaks at target sites 5' of the 5'NGG3' PAM sequence ...which nicks DNA rather than creating double stand breaks and may better facilitate homology directed repair...

  17. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...empowering researchers worldwide. Countless breakthroughs enabled by shared resources. As we step into...shared, every collaboration formed, and every breakthrough enabled brings us closer to making an impact...

  18. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...gather the results of Myriad's genetic tests for breast and ovarian cancer risk directly from customers...favorite ways to share science. Image credit: Judy Breck, via flickr  ...

  19. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...accomplishing it all. How do we encourage people to take a break from their everyday tasks and make time for special...However, I think it’s just as important to take a break from work to share time with the people around us...

  20. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...yeast research, a few of those have been trips to breweries who happily exchange our expertise with lots of...the lab, it is important to take the occasional breather and acknowledge the team’s personal and professional...

  21. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...this system, Cas9 generates a double-stranded DNA break at the genomic loci of interest and on either side...complementary to either side of the double-stranded break in the genomic loci of interest. As the universal...

  22. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...through normal mechanisms. It will take the full breadth of our research expertise, but we believe that ...health for this current COVID-19 pandemic, and outbreaks that have yet to come,” said the team from the...

  23. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...cytoplasmic retention insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation...luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation of varying...principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla luciferase...

  24. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...Conveniently, the process doesn’t cause double-stranded breaks and can deliver large sequences and even entire...allowed certain mutated phages to outcompete their brethren, and the researchers isolated these more efficient...

  26. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...stranded DNA at this sequence. The energy from this breakage is stored as a covalent bond between the cleaved... of TOPO cloning happens. Basic procedure Let’s break down the steps needed for TOPO cloning: 1. Create...

  27. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...research studies highlight the importance of taking a break and incorporating EAs into your routine.  Getting...learn new skills, while also getting a much-needed break from your lab bench.  Want further information ...

  28. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... during the day is a great way to take a mental break, while meditating right before bed will help you...it. Plan defined work hours as well as time for breaks to stretch, eat, and rest. Try the Pomodoro Technique...

  29. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field. It has the possibility...7). An independent team led by Hans Clevers at Hubrecht Institute in The Netherlands used the CRISPR/Cas9...

  30. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...it’s acceptable if it’s in the name of beer and bread. The following table lists some of the biological...end joining (NHEJ) to perform DNA double strand break repair instead of homologous recombination (HR)....
Showing: 61 - 90 of 320 results