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We narrowed to 149 results for: pcr

Showing: 61 - 90 of 149 results

  1. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...plagued by a steady barrage of problems. Why didn’t my PCR work? Why didn’t my ligation work? Why are there ... being able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where ...

  2. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...conjugates used in assays like proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020...(2007) Detecting antigens by quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://doi.org/10.1038/...

  4. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ... Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant takes...remaining starter culture can be used for colony PCR and glycerol stocks. Dip/drip plants more than once...

  7. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ... use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels of contamination...detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely available positive...

  9. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Reaction (PCR) by using a biotinylated primer. Biotin can also be enzymatically incorporated during PCR using...

  10. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic loci...and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral...

  13. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...single-stranded DNA sequence used as an initiation point for PCR amplification or sequencing. Primers can be exploited...

  14. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... your cloning experiments and tired of having to PCR many colonies to find a successful clone? Addgenie...

  15. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...DNA quality can inhibit important reactions like PCR or restriction digests. Transfection Grade DNA (high...from tissues, blood, virus, or other samples, for PCR and gel cleanup, and for extracting RNA! (That’s ...

  19. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...(nCas9). Similar to mutagenesis with error prone PCR, EvolvR uses an error prone polymerase to introduce...

  20. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...the crosslinking. Finally, purify your DNA with a PCR purification kit or with a phenol-chloroform extraction...through a variety of downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful...isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while ...

  21. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...down candidates for more specific analysis, like PCR or restriction digest. Make sure you use a proper...

  23. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...integrates our database of over 2000 primers. Two 96-well PCR plates are prepared with the primer stocks for forward...

  24. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...nuclear receptor and coregulator research. Our Q-PCR datasets, for example, document the expression patterns...

  26. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...of a downstream application such as quantitative PCR to assess gene expression. There are several things...

  27. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...Development and optimization of a real-time quantitative PCR-based method for the titration of AAV-2 vector stocks...

  28. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...reaction. This will allow you to quantify the amount of PCR product that comes from amplification of the original... titer are direct p24 measurements via ELISA and qPCR for viral RNA. Measuring p24 levels in a viral prep...first converted to cDNA and then quantified using qPCR primers targeting specific viral components such...assays depending on the vector backbone. When using qPCR based methods for viral titration consider using...most accurate method of lentiviral titration uses qPCR to measure the number of proviral copies that have...cell line known to contain 1 integrated copy of the qPCR target gene. Developing the proper control line ...

  29. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...include places where DNA is being created, such as PCR amplification or bacterial growth. Created with BioRender.com...

  30. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...screen for this specific recombination outcome using PCR and then generate a monoclonal cell line with the...
Showing: 61 - 90 of 149 results