We narrowed to 103 results for: primer

1. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...and how they work, watch Endy's 10-minute YouTube primer on BIL gates.) Addgene: Why is it so important ...

2. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   Published

   July 16, 2024, 1:15 p.m.

   ...determining titers by qPCR, we recommend using primers targeting the viral genome or inverted terminal...

3. Droplet Digital PCR for AAV Quantitation

   Type

   Blog Post

   Published

   Jan. 16, 2020, 2:18 p.m.

   ... plate containing the mastermix which includes primers and a ddPCR supermix. Note that the supermix and...

4. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...half a century; the Addgene blog has an excellent primer and tips from experts on FRET techniques. By combining...

5. Tips for a 1st time AAV user (by a Rookie AAV user)

   Type

   Blog Post

   Published

   Oct. 23, 2018, 11:49 a.m.

   ...process. Either SYBR green technology or a TaqMan primer/probe set can be used and samples are quantified...

6. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

   Type

   Blog Post

   Published

   July 8, 2025, 1:15 p.m.

   ...videos can be especially useful when designing new primers to clone your sequence of interest into Level 0...

7. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   Published

   May 20, 2021, 1:15 p.m.

   ... it worked. We pulled it off! Check out the PCR Primer video! Learn: Producing videos like this would ...

8. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   Published

   Oct. 1, 2015, 2:30 p.m.

   ...Central PMCID: PMC3032981. Resources at Addgene A Primer on Optogenetics: Introduction and Opsin Delivery...

9. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   Published

   April 16, 2020, 1 p.m.

   ... of the improvements: SHERLOCKv2 uses far less primer in the pre-amplification step allowing for greater...

10. Plasmids 101: Biotinylation

    Type

    Blog Post

    Published

    Nov. 15, 2018, 1:50 p.m.

    ...Polymerase Chain Reaction (PCR) by using a biotinylated primer. Biotin can also be enzymatically incorporated ...

11. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    Published

    Aug. 15, 2019, 12:30 p.m.

    ...rate of the organism of interest, from the day primers arrive, the desired chromosomal modification can...

12. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ...you should send it for sanger sequencing using primers that will allow you to read over the insert. See...

13. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...post, Plasmids 101: Cre-lox, if you need a quick primer on how Cre-lox recombination works. Advances in...

14. Technique: Probe Phage Genomes for Host Binding Proteins

    Type

    Blog Post

    Published

    Sept. 29, 2016, 2:30 p.m.

    ...inserts following colony PCR of 10-20 colonies using primers bordering the multiple cloning site of the vector...

15. 22 Hot Plasmid Technologies from 2014

    Type

    Blog Post

    Published

    Jan. 6, 2015, 5:21 p.m.

    ...select the appropriate pair of vector and gene primers from Table 2 of the associated publication which...of sequence homology between the vector and gene primer for SLIC cloning. Amplify the pCoofy vector and...

16. Which Fluorescence Microscopy Technique is Best for Me?

    Type

    Blog Post

    Published

    Oct. 10, 2017, 1:57 p.m.

    ...University.     Additional Resources Online Microscopy Primer on the Molecular Expressions Website Other major...

17. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...assay or screen (the Addgene blog has an excellent primer on FRET and how to measure it). Molecular mailbox...

18. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ...into the plasmid backbone, and what sequencing primers can be used to verify it. If your plasmid has multiple...

19. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...ICC and PCR genotyping (Sanger sequencing); use primers flanking the tag sequence and expect a size-shift...

20. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    Published

    Sept. 3, 2020, 12:15 p.m.

    ...Blog Read Other Optogenetics Blog Posts Read Our Primer on Optogenetics by Derek P. Simon Read Our Interview...

21. 27 Hot Plasmids from 2016

    Type

    Blog Post

    Published

    Dec. 22, 2016, 3:03 p.m.

    ...encodes a unique restriction enzyme site and unique primer sites for more rapid screening by restriction digest...

22. CRISPR 101 eBook...en español

    Type

    Blog Post

    Published

    Jan. 18, 2024, 2:15 p.m.

    ...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción...

23. Intro to Our Summer SciComm Series!

    Type

    Blog Post

    Published

    June 9, 2022, 1:15 p.m.

    ...absence of a predefined DNA sequence that uses trprimers and enzymes that will amplify a sequence only ...