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We narrowed to 602 results for: ACE

Showing: 101 - 150 of 602 results

  1. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...’t have to worry about having enough space! There’s enough space here at our new location to grow. We ... welcoming, inclusive workplace for everyone. We had Unbiasing Training in place before it was cool or...supportive Board, we have a robust committee process in place and continue to incorporate additional Board best... hard but as a 3 year Boston Globe and BBJ Best Place to Work with a positive, supportive culture, we ...

  2. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM). They're used to determine... GFP on the surface and “receiver” cells express an anti-GFP nanobody on the cell surface. When the cells...cross-reacts with BFP, sender cells expressing surface BFP generated BFP-labeled receiver cells. What’...

  3. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists ...research, and prevent technological advances from exacerbating existing inequalities and injustices. The Personal...at pgEd, I take on a variety of projects in the space of science education, communication, and policy....quickly into sensitive topics and uncomfortable places, from sticky ethical dilemmas to dark episodes ...policy requires broad interest in issues at the interface of science and society, and an ability to talk...in the legal, ethical and policy issues at the interface of biology and society.     Additional resources...

  4. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...target cell line, researchers often find themselves faced with a roadblock. In this post, we will provide ...concentrated by a variety of methods such as ultracentrifugation, centrifugation on a sucrose gradient, spin...starting. We recommend calculating the ratio of the surface area of the larger vessel to the 10 cm2 dish and...some final adjustments, this is generally a good place to start. If you don’t anticipate that you will ...relatively inexpensive and does not require an ultracentrifuge, PEG will precipitate all proteins in a solution...high-titer lentivirus preparations without ultracentrifugation. Sci Rep. 2015; 5:13875. PubMed PMID: 26348152...

  5. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic acid, ...they are not currently provided. Eye protection or face shields are required whenever there’s a splash or... and removed in a biosafety cabinet. For ultracentrifugation, heat sealed tubes can be used for extra ...decontaminate after use. Virus-exposed plastics and other surfaces should be thoroughly decontaminated after use,...basically, you’ll need to get trained in each new place (and pay attention to the trainings!), even if you...

  6. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ... by ultracentrifugation (75,000 - 225,000 g for 1.5 – 4 hours at 4 °C). After ultracentrifugation, virus...vectors) you can concentrate your virus by ultracentrifugation of the viral stock, followed by resuspension...packaging cell debris before proceeding to ultracentrifugation to avoid contaminating your transduction ... to concentrate a virus solution without ultracentrifugation, you can reduce the volume of cell culture...exchanging the medium after transfecting cells, replace the medium with half the volume typically used ...

  7. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...student at the Max Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will...Clutter not only makes it difficult to clean your workspace properly, but can also disrupt the laminar air...materials into the hood. This will give you enough space to move around freely without causing spills. Also... than 80-85% confluent because cells like their space. If they reach confluence, they stop growing (contact...cells stored in their original condition and can replace your working stock if it gets too old or contaminated...lab at the Max Planck Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology...

  8. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ... Specially constructed plasmids can be used to replace genes in yeast, mice, or Drosophila through homologous...targeting takes advantage of this natural process to replace a targeted genetic locus with homologous sequence...gene will be removed from the chromosome and and replaced by the resistance gene.The gene is thus disrupted...recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted gene is disrupted ...normally because one of its exons has simply been replaced with the same sequence flanked by LoxP sites while... while the resistance cassette has been placed into an intron.    Figure 4: A targeting vector...

  9. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ... they not only traced the 3-D path of the protein’s backbone in three-dimensional space, but their flexible...ribosomal subunit, he kept sliding his palm across the surface of the protein where the nascent polypeptide emerges...realized how perfectly flat the complex is on this surface”….where the complex is docked on the membrane of...serious way. We represented the Cas9 protein in a surface format made of rock-hard, inflexible plaster. As...primed for cleavage. This structure captures the displaced non-target DNA strand and shows the HNH nuclease...

  10. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...in-house software has been crucial for us to keep pace with our current need to analyze nearly 300 sequences...sequencing data collection and analysis eventually outpaced our software, even with continuous updates. The...other bioinformatics purposes  Integrated user interface, so our QC team could initiate and monitor the...open-source sequence assembly tools at the appropriate place in our workflow without having to translate or convert...Biopython.  We started at the usual recommended place: documentation. We oriented ourselves with the basic...smoothly operate the pipeline, we needed a user interface. In our next post, we’ll share our story about...

  11. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...of common genetically encoded neuronal tracers Neuronal tracers such as epitope tags and fluorescent proteins...Spaghetti Monster - A new and improved neuronal tracer With the limitations of epitope tags and fluorescent...fluorescent proteins, it has been crucial to develop new tracers that enable accurate labeling of neurons. As discussed...Addgene Uses of smFPs 1. Multi-channel connectomic tracers Fluorescent proteins such as GFP have been used...labeling experiment using smFPs and standard neuronal tracers. 2. Visualization of neuronal sub-cellular structures...

  12. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

    Type
    Blog Post
    Published
    Dec. 2, 2021, 2:15 p.m.
    ...simply embellish and adorn your lab space or your work from home space in a fun, festive holiday or winter...winter or holiday theme decor in your work from home space… but with a science twist. Maybe you have a collection...using life science catalog pages. Make your WFH space feel a little more festive this holiday season! ...Instagram as we share pictures of our decked out spaces.       ...

  13. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...information is consolidated to one place, easily retrievable, searchable and traceable. However, switching from ...that bring more to the table in terms of data traceability and reproducibility of research. Electronic ... enables researchers to store everything in one place. From pictures to excel sheets, researchers today...enable researchers to keep all their data in one place and avoid scattering intellectual property across...run, ELNs enable labs to keep their data in one place and therefore make it easier for them to track and...

  14. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...non-scientists alike will march to advocate for science’s place in education, government, and civilization in general...attribute that enables science: “humility in the face of evidence” (4). Frustration among scientists has...aspects of research at National Aeronautics and Space Agency, the Environmental Protection Agency, and...inclusion of science) they come from many different places supporting many different positions. It would be...and 514 satellite marches are scheduled to take place around the world. There is no requirement that marchers...plan Science Magazine: NIH, DOE Office of Science face deep cuts in Trump’s first budget Environmental ...

  15. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...(Brinkman et al., NAR). This technique uses the trace file generated in Sanger sequencing and a simple...Fig. 1: Overview of TIDE. The input is sequencing trace files of WT and edited cell lines. Then decomposition...and your Cas9-targeted cells. Simply upload both trace files to the TIDE tool online along with your sgRNA...outlined above), except that it uses a 3rd sequencing trace file from the donor DNA. This method can precisely...edited sequence would look like (the 3rd sequencing trace). While it requires a little extra wet lab work,...quantitative assessment of genome editing by sequence trace decomposition. (2014) Nucleic Acids Research Volume...

  16. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...versions obsolete, the techniques we use in lab are replaced by improved versions that save both time and money...observed. Today Southern blotting has been largely replaced by real-time PCR to answer the same experimental...unknown sequence. In the era before sequencing was commonplace, researchers used restriction mapping to investigate...this technology, radiolabelled nucleotides were replaced with fluorescently labeled ones allowing for fluorometric-based...discoveries (5, 8). While newer techniques have largely replaced these, these techniques still have useful applications...to recombinant DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly...

  17. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    Published
    April 5, 2016, 2:30 p.m.
    ...world continues to face global challenges, we hope to empower those that need to face these challenges ...global challenges. However, these same researchers face significant barriers to scientific discovery and...near future, we hope to eliminate much of white space on this map and increase plasmid sharing across ...Follow Seeding Labs on Twitter @SeedingLabs or on Facebook Additional Resources on the Addgene Blog Learn...

  18. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...interesting, much of what a phage faces as it approaches a host cell surface are complex polysaccharide structures...amplification (by a mechanism known as multiple displacement amplification) even in the presence of certain...colonies overnight), flip the membrane over and place it colony-side up onto a fresh LB plate containing... is done, peel the membranes off the plates and place each onto a lysis solution-soaked filter paper (... blocking solution (around 108 CFU/mL is a good place to start) and add your membranes to the solution...

  19. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...your Addgene account.  Orders do not need to be placed immediately upon request approval. Once approved...request will remain in your account until you have placed your order. You will only be charged once you have...  What happens after I order?  Once an order is placed, the Addgene viral vectors team gets to work! They... before you order.  Timeline Once your order is placed, we estimate it will take 6-8 weeks for the service... you’ll be able to work with the distributor to place a request and order if approved.  I want to stay...

  20. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...researchers from different locations. Having virtual face-to-face meetings as opposed to phone calls most closely...feel included in the project and accelerates the pace at which problems can be addressed and new ideas...that work best for them. This also accelerates the pace of research by circumventing the slower, sequential...Scientific conferences and workshops are another great place to meet up with collaborators. Being able to present...

  21. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...Biorender workspace. Free Biorender accounts can store 5 workspaces at a time and workspaces are only ...It’s part of Google Drive so it has a similar interface as Google Docs or other products in the suite....collection and drag and drop an icon onto the workspace. The color and size of icons are adjustable. New...

  22. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...These scaffolds are stretches of non-coding RNA interspaced with linkers that can fold into a secondary structure...this new scaffold RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit...five genes that encode biosynthetic enzymes for violacein, a distinctive pigmented protein. By altering ...generate differently pigmented derivatives of violacein. Using dCas9 as an on/off switch The multiple ...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...

  23. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...Archives allots for 25 MB of space per user, but, if you need more space, you can sign up for a plan. ... Many thanks to our guest blogger Sean Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State...This post was contributed by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article ...

  24. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ... loaded onto cesium-chloride gradients and ultracentrifuged for up to 20 hours to separate the intact ...have, access to one or more large, expensive ultracentrifuge.  Figure 1: An outline of the different...out to develop a protocol that skipped the ultracentrifugation altogether. Chomczynski and Sacchi showed...cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform are added to the homogenate and...shaken. After a quick centrifugation (not ultracentrifugation!), the layers of phenol and chloroform separate...

  25. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... enzyme 2 (ACE2) cell-surface receptor. The spike protein RBD responsible for binding ACE2 is the target...SARS-CoV-2 spike RBD is displayed on the yeast cell’s surface. The Bloom lab used the library to identify RBD...RBD mutations which do not interfere with ACE2 binding, but impact recognition by specific anti-SARS-CoV...

  26. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...the secretory pathway to their subcellular or extracellular destination. At the end of their life cycle,...to be a part of an organelle or involved in intracellular transport pathways, it is possible to map the... in order for the antibodies to reach their intracellular targets. Therefore, it can be beneficial to ...Transfer, FRET. Additional opportunities: Tracking intracellular pathogens In addition to tracking the location...possible to follow the life cycle of viruses and intracellular bacteria – have a look at our Microbiology resources...

  27. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...to almost every person regardless of profession, race, or gender. Almost no other disease receives as ...divide only at the right times and in the right places. Checks and balances: protecting against cancerous...Luckily, there are several safety mechanisms in place to protect us from rogue cells when something does...variety of cancers, Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult...Resistance with a New Screening Kit Learn How the Clon Tracer Library Can Be Used in Cancer Lineage Tracing ...

  28. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...volunteering, it gave me a place to go once a week to get recharged. The fun change of pace helped break up the... great way to share some of the research taking place at our university with the community. I even spray...interact with their followers. It’s also been a great place to reach out to local establishments such as museums...provide plenty of negativity, so I volunteered at a place that made me happy. I started volunteering in the...

  29. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...REPAIR (RNA editing for programmable A to I (G) replacement) is the first CRISPR tool for RNA editing. Two...enzymes prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like...be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine if...PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated ...they confirmed A->I editing and found that 50 nt spacers increase editing efficiency but also increase off-target...

  30. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...your science, one of the big questions you'll be faced with is "how?" How are you going convey your information...learned (somewhat successfully) how to text or use Facebook. However you choose to communicate your science...interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication... lectures, presentations, etc… Low (except for space) Real-time audience engagement and response. High...fear. Can be discouraging to build audience. Need space & higher entry barrier for audience versus passive...

  31. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...event-planning base and will know how to reserve space, time events, and network for volunteers. Speaking...school’s Math & Science Night. There are plenty of places to get ideas for science activities. I did some...curriculum, would be hard-pressed to maintain the pace of CRISPR. Two of the examples I used in my presentation... considered proficient in science, regardless of race or ethnicity. Perhaps you don’t have children but.... Maybe you just like to see the look on a kid’s face when you’ve shown them how to isolate DNA from a...

  32. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...AdV surface proteins (especially fiber’s knob domain, and hexon), as well as other intracellularly-exposed...pediatric population. Once ~400 cases had amassed in places around the world, hypotheses began to develop that...AdV genome in them, including the E1 region). The space in the viral particle’s capacity that is made available... G. R. (2015). Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6)...

  33. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Production Recombinant antibody production can take place in bacterial, yeast, plant, or mammalian cells. ...variable chains are secreted into the periplasmic space to be oxidized and form disulfide bonds (Frenzel...does not, be sure to set the absorbance to 280 nm (Pace et al., 1995). You’ll also need to know the extinction...10.1002/bit.27776. Epub 2021 May 4. PMID: 33811659. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T. How to ...characterization & rheology, Current Opinion in Colloid & Interface Science, Volume 19, Issue 5, 2014, Pages 438-449...

  34. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...Jonathan Gootenberg, PhD, focuses exclusively on this space — developing tools for cellular targeting. Lately...and cleaving RNA at positions defined by the target:spacer duplex without detectable non-specific activity...mutations wouldn’t matter – the mutated copy could be replaced by simply dragging and dropping in a functional...allowing a one-size-fits-all approach to gene replacement therapy for most patients. But how does one go...chance of the insert being “dropped” in the wrong place, while increasing the number of potential applications...

  35. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...vectors, you need an envelope protein on the virus’s surface and a corresponding receptor in the host cell. ...low-density lipoprotein receptors (LDLRs) on a cell surface to endocytose into the cell. The reason VSV-G is...preparation, VSV-G is stable and can withstand ultracentrifugation which helps scientists achieve a high titer... rabies glycoprotein now serves as a retrograde tracer so that scientists can identify the neurons that...2001).  This method can also allow scientists to trace a neuron’s projections back to the cell body. Rabies...

  36. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...commonly associated with CRISPR arrays and involved in spacer acquisition following infection, as a form of “...is reduced when 2 mismatches are present. The protospacer flanking sequence (PFS) for LshCas13a, which ...sequence for Cas9, is located at the 3’ end of the spacer sequence and consists of a single A, U, or C base...each DNA target have high sequence identity to the spacer sequence and contain the appropriate PAM sequence...cells. LwaCas13a-msfGFP can mediate cleavage with spacers from 20-28 nucleotides in length and does not require...

  37. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ... public speaking where I enjoy communicating face to face with the audience. I post on LinkedIn where ...thoughts on why we decided to do an AMA in the first place and give you some reasons why you should consider...realized that the r/Science AMA series was the best place for us. We’re definitely glad we did - you can see...lot of links and content at hand in three useful places: I had posted content on many of the requested...

  38. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...nose-poke at the active hole [1, 2].    Place preference/place aversion also models reinforcement but ...include: intracranial self-stimulation (ICSS) and place preference. The lab I work in (the Kreek lab) focuses...ideal behaviors to test reinforcement are ICSS and place preference.   Historically, ICSS was done by implanting...hippocampal neurons are activated when the mouse is placed in a novel context. As a proof-of-principle test...

  39. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ... to you: our first, second, and third place winners!  1st Place: Krampus Saves Christmas! created by the... Xi Chen (@XiChen7889) December 10, 2021   2nd Place: The Winter and the Falcon Tube created by the team...Heng Chooi (@YitHengChooi) December 4, 2021   3rd Place: O! Beaker Tree Created by Joanne Jary from Lab ...

  40. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...activation p300 acetyltransferase dCas9 fused to the catalytic domain of p300 acetyltransferase increases levels...modifications, researchers used blunt tools like histone deacetylases, but targeted epigenetic modification was impossible...light-regulated histone methyltransferases and deacetylases. CRISPR and epigenetics As with many TALEN-based... Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers...

  41. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...culture through various sources that are difficult to trace. These include the laboratory personnel, the serum...the flasks (unless the source of culture is irreplaceable or highly expensive). Detecting mycoplasma contamination...mycoplasmas, contamination by other bacteria or extracellular fluorescent signals produced by these bacteria...culture flasks. In cases where the culture is irreplaceable or very expensive, there are some ways to rescue...cultures because you are not the first person to face this kind of problem. Educate others when you can...

  42. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...important of those is to always look closely at the trace file (or chromatogram) of the sequencing results...sequence (no Ns in sight). If you never looked at the trace you would be happy. But look closer, the overlapping... 2. You should see individual, sharp and evenly spaced peaks …like these…. 3. Expect to get 500-700 bases...you can purify the product either via the Sodium Acetate/isopropanol precipitation method or using a silica...

  43. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...or by project, if that works better for you) and place them on an easy to reach shelf. Make sure all notebooks... Post-its and write brief descriptions on each. Place the notes sticking up out of the top of the notebooks... notebooks (ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to...find to where the binder was moved. You’ll have peace of mind knowing the information is backed up and...

  44. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever more... structure to interrogate the sequence isn’t in place yet.     Figure 4: S. pyogenes Cas9 in the...perfect guide RNA will do you no good if there is no adjacent PAM to anchor it. However, Cas9 only checks the...HNH domain, ensuring all binding partners are in place before cleavage can occur (Jiang & Doudna, 2017;...

  45. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...talk about Addgene’s internship opportunity taking place next summer.  I applied to Addgene and began the...first week at Addgene, I had to get used to the workspaces, as well as complete the lab safety and cybersecurity... clones because the bands were all in the wrong place. Except for one. My manager speculated that the ...safety, general work skills — such as using Google workspace — and got the chance to work on my very own research...

  46. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...best place for the biosensor to be expressed. Are you looking at an intracellular or extracellular molecule...calcium sensor CMV-mito-GEM-GECO1  and blue flame extracellular glutamate sensor pCMV(MinDis).iGluSnFR. Picking...

  47. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...special words about managing scientists because, let’s face it, we are special. Below are some special characteristics...characteristics of scientists in the work place as described by Sebastiano Massaro in his Nature Biotechnology...approached as peers rather than subordinates Need mental space and dislike intrusions (this actually varies, a ...scientists) Are highly mobile and can move to a new workplace if opportunities for learning and personal growth...

  48. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...science writing in magazines or newspapers or other places online, and consider how those stories are being...their blogs. 5)   Local science museums are a great place to find opportunities for science outreach. Here...institution; the news or communications office is a good place to start. It’s not unusual to find science writers...Mass Media Fellowship or maybe an internship at a place like Science News. Ask your institutional news office...

  49. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex...blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes...genes. These data highlight an interesting "arms race" between phages and prokaryotes; the ultimate victor...

  50. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...some marketing experience, with little emphasis placed on the marketing or business background of the ...focus on the list of conferences I presented at and placed them under headlines entitled “Presentation Experience...more than necessary for my interview. The simplest place I could think of to start was “Marketing for Dummies...outside of academia, sales and marketing can be great places to start. Depending on what type of career you ...
Showing: 101 - 150 of 602 results