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We narrowed to 444 results for: Ank

Showing: 101 - 150 of 444 results

  1. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...way. Many thanks to our guest bloggers, Samantha Zyontz and Neil Thompson. Special thanks to Addgenies...

  2. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...gene-edited babies. Several hours later, the scientist, Jiankiu He, confirmed that two such edited babies were ...the necessary discussions and actions now.  Many thanks to our guest blogger, Kelly Hills! Kelly Hills...

  3. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...paper lab notebooks you’re saving trees! The Earth thanks you. 2. Gibco Cell Culture by Gibco Whenever I ...please share it in the comments section below! Many thanks to our guest blogger Sean Stacey! Sean D. Stacey...

  4. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...buffer, which will be noted on the product sheet. Thankfully, sodium azide can easily be removed from the ...complex subjects in antibody-based protocols - but thankfully this is an Antibody 101 post, so we'll keep it...

  7. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...multidisciplinary effort and cross-team collaboration (thanks to all Addgenies involved). The new homepage and...example, matches of a name in the depositor field will rank higher than matches of the same name in the publication...

  8. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...Find pSpCas9(BB)-2A-Puro (PX459). Thank you! We would like to thank all of our depositors and requestors...

  9. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...authors are asked to add an identifier (a bit like a GenBank ID) to all antibodies, animal models, and software...publishing your manuscript, or add your own tips below. Thank You to Our Guest Blogger! Maaike Pols is a Developmental...

  10. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ... provide detailed evidence of knowledge new to mankind. And you probably understand how much faster it...communicate. It’s okay (and encouraged) to leave blank spaces or to gloss over some aspects when needed...

  11. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ... lost in a sea of data, my mind went completely blank. After several seconds of truly panicked silence... so they could understand it. When my mind went blank, my options were to stop talking until I could present...

  12. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...and can elute proteins with up to 95% purity (Janknecht 1991, Hochuli 1988). In this purification approach...Adsorbent. Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff G, Lou J, Hipskind RA, Nordheim...

  13. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting single...use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting single stranded...

  14. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...library was developed when Carrie was a post-doc in Frank Stegmeier’s lab in Novartis Oncology.  Bhang did...ways you put the ClonTracer library to use! Many thanks to Carrie and Novartis for taking the time to talk...

  15. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...majority of the visual data. This central box is then flanked by two equally-sized columns, where the introduction...sections like the introduction, methods, and figures flank the central region, and a scannable barcode is included...

  16. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...site within the genome using homology arms that flank the gene of interest. In either case, a positive... sites are inserted directly into the genome to flank the gene that will be deleted following Cre-mediated...

  17. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...whole segment—a GFP tag and 1kb of homologous DNA flanking both sides (about 2.7kb total)—is inserted into... use genomic tagging in your experiments. Many thanks to our guest bloggers from the Allen Institute ...

  19. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...isolated from two patients with cystic fibrosis (Schwank et al). They were able to demonstrate functional...Cas9." Cell Stem Cell. 13, 659-662 (2013). Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9...

  20. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...

  21. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...deliver light pulses to the target brain region. Thankfully, due to their prominence, the preparation of ...performing optogenetics behavioral experiments. Thank you to our guest blogger Derek Simon! Derek P....

  22. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...at this business and started things up for real: bank account, business cards, incorporation, laboratory...this transition (so far) and what we’ve learned.  Thank you to our guest bloggers! Nikolai Braun grew up...

  23. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...plasmid that contains the Mobile CRISPRi cassette flanked by left and right Tn7 end sequences. Donor bacteria...in the recipient cell, the CRISPRi DNA (purple) flanked by left and right Tn7 end sequences (green) integrates...

  24. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ... spent organizing, an hour is earned (Benjamin Franklin)! It’s good practice to label your plates and/...autoclaved before being disposed Happy culturing! Many thanks to our guest blogger, Sana Khan Khilji! Sana Khan...

  25. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...transmitting and sensing electrical potential. Thankfully, there is a tool to measure these electrical ...specific types of cells, such as neuron subtypes, thanks to viral serotype specificity during delivery (...

  26. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...alterations are achieved through DNA degradation, thanks to the unique capabilities of Cas3 and its partner...CRISPR type I-E. Created with BioRender.com. Thanks to this complex, developing Cas3 tools has been...

  27. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...because of our CRISPR depositors and requestors. Thank you!  Want to dive deeper into Addgene’s CRISPR ... The CRISPR Journal (Pyhtila et al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions...

  28. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...cells. These plasmids use fluorescent proteins flanked by long regions of homology to the gene of interest...and protein-protein interactions. Additionally, thanks to the modular arrangement of vectors included ...

  29. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...provide them with a CV or transcripts if requested Thank them, and eagerly await a reply. It’s a good idea...interest. Benefits of Online and Hybrid Options Thanks to the much more developed virtual environment ...

  31. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...depositors to provide plasmid maps whenever possible. GenBank files can also be uploaded onto our plasmid pages... resources provided by the depositor including genbank files and protocols here. Articles Citing this ...

  32. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...green fluorescent protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (... system under normal and diseased conditions.  Thank you to our guest bloggers, Jae Lee and Pantelis ...

  33. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...and single guide RNAs (sgRNAs) with appropriate flanking homology into a germline cell can create an RNA-guided...drives can be found in the referenced publications. Thank you to our guest blogger! An inventor of technologies...

  34. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...know how it goes! Acknowledgements: I’d like to thank the PTA of Butler Elementary for trusting me with...with Math & Science Night, and a special thanks to Joanne Marks, Jonathan Marks, and Vanessa Bullard of...

  35. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential viral genes. How it...blog was written by Addgenie Brian O'Neill, with thanks to Addgenie Rebecca Hutcheson for her assistance...

  37. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats (LTRs) that facilitate...Bernaudin F, Girot R, Dorazio R, Mulder GJ, Polack A, Bank A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel...

  38. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...These primers are designed to anneal to sites that flank the insert site. A positive clone will produce a...this type of primer pair anneals to a sequence flanking the insert and one primer anneals to the insert...

  40. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...in-vivo biotinylation signaling peptide (Bio), flanked by hexahistidine motifs (6xHis) MBP Large Protein...Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his wife, Annette Sievers, Ph.D., for her tips ...

  41. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...018-1591-1 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing...

  42. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...large formats, such as 96-well microplates, and thanks to our dedicated product and development teams,... shared resource of pipelines for genomic data. Thanks to the flexibility inherent in Nextflow’s design...

  44. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...possible facing the committee and not the whiteboard. Thank everyone for the opportunity to share your research...the opportunity to realize your vision. Many thanks to our guest blogger, Erik Snapp! Erik Lee Snapp...

  45. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ..., One Drive, and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional...reproducibility as not all ELNs are created equal. Many thanks to our guest blogger, Rory Macneil!  Rory is founder...

  46. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...GKPIPNPLLGLDST) has been used as a tag since 1992 (Hanke et al., 1992), and has proven itself a versatile...Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski, P., & Randall, R. E. (1992). Construction...

  48. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct repeats which can be used for marker...through a second recombination event between the two flanking repeats. In cells where recombination has not ...

  50. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...postdocs in the final ruling was, for a lot of us, frankly quite surprising. Consortia of higher education... changes if we wish to minimize the hurt. Many thanks to our guest blogger Gary McDowell! Gary S. McDowell...
Showing: 101 - 150 of 444 results