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Addgene
Showing: 41 - 60 of 205 results

  1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ... space of a standard pol III-gRNA, which will facilitate CRISPR/Cas9 multiplexing in systems with limited...overlapping activity. Multiple gRNAs can also facilitate deletions of other regions, such as noncoding...

  2. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...detect expression of toxin mRNA from Clostridium difficile. As mRNA expression cannot be identified with ...infection, dysbiosis, and antibiotic treatment. To facilitate these studies, Pamela Silver’s lab developed ...

  3. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...utilizes a Cas12f from a different species, Acidibacillus sulfuroxidans, AsCas12f1. They found that this...protein PEG10 specifically binds its own mRNA to facilitate its packaging into virus-like particles. Feng...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...to start an open science nonprofit that would facilitate the sharing of plasmids between scientists. The... r/labrats, Reddit.  Part of that work meant facilitating the Material Transfer Agreement process, which...

  5. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ... you will be doing your research in a group or facility that is a world leader in your chosen area of ...Learn How to Become a Grant Writing Professional Facilitate Career Planning and Personal Development with...

  6. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ... broaden the conversations about genetics and facilitate dialogue between scientists and communities. ...With our focus on engaging the community and facilitating dialogue, a major part of our everyday work ...

  7. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...Future of Science, and the MIT Graduate Student Council will be held on Friday 16th and Saturday 17th September...Future of Science and the MIT Graduate Student Council, we shift our focus toward advocacy and action ...

  8. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...bacterial integrative and conjugative elements in Bacillus subtilis (ICEBs1) system. ICEBs1 carries the genes...ICEBs1, an integrative and conjugative element in Bacillus subtilis." Plasmid 86 (2016): 14-25. PubMed PMID...

  9. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...There are several facilities nationwide where you can obtain excellent quality, high titer rAAV (including...like adenovirus (Ad) or herpes simplex virus to facilitate efficient replication. The most common production...

  10. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...most promising pegRNA designs and especially facilitates the use of prime editing in pooled settings with...models (such as PRIDICT/PRIDICT2.0) can greatly facilitate prime editing experiments, thus saving you time...

  11. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...What do a viral vector production facility, food allergy testing lab, and the grad student down the hall...-to-day work. Indeed, viral vector production facilities frequently use qPCR with a standard curve to ...

  12. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...flanked by HIV-1 long terminal repeats (LTRs) that facilitate viral packaging and host genome integration. ...transcriptional activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral...

  13. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...attached to a protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific...tolerance, and optional fluorescence of FPs with facile antibody recognition of epitope tags. Thus they...

  14. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    .... In vivo, these recombination reactions are facilitated by the recombination of attachment sites from...attL-entry-TOPO vector. TOPO cloning adds short end(s) to facilitate cloning into an attL-containing entry vector....

  15. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...’ resistance to beta-lactam antibiotics, like penicillin. These specific drugs attack the cell wall of... of survival. This was the whole basis of how penicillin was discovered! Of course we wouldn’t have such...

  16. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...Yersinia pestis, Agrobacterium tumefaciens, and Bacillus anthracis (Lui et al., 2012, Ni et al., 2008, ...Zhu L, Wang H (2012) Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility. PLoS ...

  17. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...screens Improved prime editor PE7 Antibodies to facilitate SARS-CoV-2 research New viral vector preps of...-024-07259-6. PMID: 38570691. Antibodies to facilitate SARS-CoV-2 research   By Ashley Waldron Ready-to-use...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...to either cytosine or adenosine deaminases to facilitate a single base pair edit. (Cytosine deaminases...of activators include the SunTag system which facilitates recruitment of multiple activation domains to...

  19. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...Syndrome research. With a shared passion for facilitating research and discovery, Addgene and RSRT have... share additional resources. Plasmid tools to facilitate MECP2 curative strategies, such as CRISPR and...

  20. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...Biological Sciences Research Council (BBSRC) and Medical Research Council (MRC) require authentic cells...
Showing: 41 - 60 of 205 results