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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 361 results for: ERG

Showing: 41 - 60 of 361 results

  1. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...light-sensitive proteins that absorb photons for energy conversion or the induction of intra- or intercellular...shorter than 5 kb (Hirsch et al., 2016; Hudry and Vandenberghe, 2019). How to achieve strong opsin expression...PubMed PMID: 26135556. Hudry, Eloise, and Luk H. Vandenberghe. "Therapeutic AAV gene transfer to the nervous...Juarez, Barbara, and Ming-Hu Han. "Diversity of dopaminergic neural circuits in response to drug exposure...

  2. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...emitting a photon with slightly less energy due to non-radiative energy losses. This difference in emission...ensure accurate tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes need to ...excitation and emission profiles to enable effective energy transfer, which is key to observing interactions...

  3. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

    Type
    Blog Post
    Published
    Nov. 29, 2018, 1:53 p.m.
    ...discovered and studied but it is only the tip of the iceberg as it has been predicted that many others are still...microbial genome and metagenome from the Department of Energy’s Joint Genome Institute for uncharacterized genes...gene together with the adjacent CRISPR array and intergenic regions containing the putative tracrRNA into...

  4. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ... its confines. The beauty of serendipity often emerges at the intersections of disciplines. Cast your ... capture fleeting thoughts and connections that emerge during moments of inspiration. Additionally, journaling...serendipity. However, the best discoveries often emerge when we allow ourselves the freedom to explore ...

  6. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...sequences. Repeating sequences within a plasmid can undergo intramolecular DNA recombination. This naturally...two repeating elements love each other very much undergo recombination, two smaller plasmids are formed....To reduce recombination, it is important to not overgrow the plasmid at 37°C. Some researchers find that...

  7. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ... An undergraduate can mentor a high school student, a graduate student can mentor an undergraduate, a ... up on plans. Make a commitment to put time and energy into your mentoring relationships. You will only...

  8. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...non-governmental groups with seeking “public views on emerging IVF techniques designed to prevent the transmission... a controversial and ethically fraught rapidly emerging technology. We could do this for CRISPR/Cas9 –...in the software industry to inform her work on emerging issues in novel technologies, including self-driving...

  9. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...Instead, R body monomers respond to pH changes by undergoing local conformational changes. These tiny movements...decades, and they were known to be resistant to detergents and degradation under a variety of conditions...conditions. More speculatively, R bodies might endow divergent organisms with new functions. For inspiration,...

  10. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...ll provide information about commonly used and emerging capsids to help you decide which ones may be suitable... of published descriptions of commonly used or emerging capsids. Selected capsids are further highlighted... regional bias towards the cortex and thalamus emerges. AAV-PHP.eC effectively transduces neurons and ... Moncada-Reid, C., Eid, F.-E., Walkey, C. J., Ljungberg, M. C., Lagor, W. R., Heaney, J. D., Chan, Y. ... Sakopoulos, S., Schneider, G., Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken, T. P., Smith...

  11. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...just such a system, which they call CRISPR/Cas9 Synergistic Activation Mediator or SAM. SAM is an engineered...distinct effector domains that work together synergistically, modeled after natural transcription activation... J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2015...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...cargoes, genetically engineered neurotropic viruses emerged as powerful tools for visualizing neural connectivity...monosynaptic tracing to identify inputs to two major dopaminergic cell populations in two specific brain regions...617–623. Pubmed PMID 19349161.  2. Deshpande A, Bergami M, Ghanem A, Conzelmann KK, Lepier A, Götz M, Berninger...

  13. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes a conformational change that permits it to bind... samples. The Barrick lab and the UT Austin undergraduate iGEM team originally used a guaB knockout E....can be used in quenching, FRET (Förster resonance energy transfer), and FLIM (fluorescence lifetime imaging...

  14. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...obstacles when working with virus: 1. Viral vectors undergo DNA rearrangements. Depending on the composition...repeat sequences present in the LTR, the vector may undergo genomic rearrangements during replication. To avoid...immunotherapy." Acute Leukemias VIII. Springer Berlin Heidelberg, 2001. 291-306. PubMed PMID: 10634178. 8. Murnane...

  15. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ... media, while others remain cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo...rAAV sample has to be digested with DNase before undergoing titering, to remove residual plasmid DNA carried...Article 10. https://doi.org/10.1089/hum.2009.223 Vandenberghe, L. H., Xiao, R., Lock, M., Lin, J., Korn, M...

  16. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...Manzo, S. G., Peric-Hupkes, D., van den Berg, J., Beijersbergen, R. L., Medema, R. H., & van Steensel, ...02268-2 Schep, R., Brinkman, E. K., Leemans, C., Vergara, X., van der Weide, R. H., Morris, B., van Schaik...

  17. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ...I knew that when I was “managing” a couple of undergrads back in grad school. Both were hard-working scientists-in-training...reviewing the results of my Myers-Briggs test:  my undergrads were different people! More specifically, they...time I could likely have retained this talented undergrad. The point here is managing is not “one size ...

  18. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said “I hadn’t...accurately suggests the potential of this protein to undergo conformational shifts. This backbone model of Cas9...conformational gymnastics that this protein must undergo as it interrogates your genome. If you are interested...

  19. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...takes roughly 10 days for an egg to develop and emerge as an adult fly at 25°C. At low temperatures like...the day and throughout daytime. They mostly stop emerging at night. An incubator with a light cycle makes...makes for very predictable work! When flies first emerge, their bodies will be very light in coloration ...

  20. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...their viruses; food allergy labs use standard curves with ELISAs to measure allergen concentrations in ...What do a viral vector production facility, food allergy testing lab, and the grad student down the hall...
Showing: 41 - 60 of 361 results