We narrowed to 51 results for: HDR

1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ...edit a gene using homology directed repair, since HDR-mediated gene editing is most efficient when target...

2. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

   Type

   Blog Post

   Published

   July 7, 2020, 1:15 p.m.

   ...protein) upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et al., 2016). For TREE they engineered...

3. Site Directed Mutagenesis by PCR

   Type

   Blog Post

   Published

   Aug. 2, 2016, 2:30 p.m.

   ...endogeneous DNA through homology-directed repair (HDR) of a CRISPR/Cas9 induced double-stranded break. ...

4. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous genes close to...

5. Hot Plasmids: Spring 2025

   Type

   Blog Post

   Published

   June 3, 2025, 1:15 p.m.

   ...than methods relying on homology-directed repair (HDR), and insertion is scarless, unlike previous integrase-based...

6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

   Type

   Blog Post

   Published

   Oct. 11, 2016, 2:30 p.m.

   ...These precise edits tend to be more efficient than HDR (reviewed in Zhu et al., 2019). Both cytosine base...

7. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

   Type

   Blog Post

   Published

   Nov. 3, 2015, 3:30 p.m.

   .... These tools assess repair events by type (NHEJ/HDR) and track indels at a single locus, usually the ...

8. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

   Type

   Blog Post

   Published

   Sept. 28, 2020, noon

   ...eukaryotes in that Cas9-mediated cleavage induces HDR to directly modify a given gene. CRISPR-tranposons...

9. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   Published

   Aug. 5, 2021, 12:15 p.m.

   ...Elizabeth Hubbard: Neurons involved in opioid withdrawal Elizabeth Hubbard is a fourth year Ph.D. candidate...the development of behavioral signs of opioid withdrawal before they occur.  First, she used a genetic...genetic method to tag neurons activated by opioid withdrawal with tdTomato and assessed cFos expression. She...fluorescence during morphine administration and withdrawal to explore how these cells may encode the negative...negative affect associated with drug withdrawal.  “Together, this project will allow us to better understand... how neuron activity changes following opioid withdrawal, providing insight into new methods for treating...

10. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type

    Blog Post

    Published

    July 21, 2016, 2:30 p.m.

    ...Office, between 1997 and 2000, ten drugs were withdrawn from the US market because of life-threatening...therapies. Let’s go back to why ten drugs were withdrawn from the market between 1997 and 2000. There are...

11. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ...indicating a quick cell cycle. Cells that had IL-3 withdrawn became entirely red as they could not proliferate...