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We narrowed to 253 results for: IRES

Showing: 41 - 60 of 253 results

  1. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...it like winter tires in different conditions: the more flexible rubber of winter tires give a better grip...recombinant proteins for industrial or research purposes requires stable host cells (the simplest example would ...

  2. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...are a non-renewable reagent - Their production requires animal sacrifice and because they are an animal...secondary Less sensitive than HRP-RANbody staining Requires secondary antibody staining HA Tagged GFP, RFP...secondary   Less sensitive than HRP-RANbody staining Requires secondary antibody staining GFP, RFP, H2A2B ...

  3. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...of these orthologs is key: since each ortholog requires a different PAM sequence, a gRNA designed for ...color is specified by PAM sequence, each color requires a different Cas9 ortholog, and the target sequence...in combination with gene editing. CRISPRainbow requires very short, 11-mer gRNAs, which do not induce ...

  4. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ... “first generation” CRISPR activator. While it requires a relatively simple construct, it exhibits modest...activation, dCas9-VP64 is great for experiments that requires modest gene activation. Synergistic Activation... to other notable CRISPR activators is that it requires a fusion protein, rather than relying on a two-component...

  5. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ..., comprised of PAS and GAF domains of iRFP713. Requires presence of heme oxygenase for chromophore formation...miRFP709 and miRFP670, pair with common PAS domain. Requires presence of heme oxygenase for chromophore formation...BiFC interaction is typically irreversible and requires some time for chromophore maturation, splitFAST...

  6. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ... Park.  In addition to Taq DNA polymerase, PCR requires free nucleotides (dNTPs), template DNA to amplify...measuring a starting DNA concentration using PCR. qPCR requires the addition of a probe based fluorescent dye ...transcription-qPCR (RT-qPCR). The first step of this process requires RNA to be converted to cDNA using reverse transcription...

  7. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...handled at BSL-1, while lentivirus requires a BSL-2 setup. This requires a less involved setup when working...Created with BioRender.com. The in vitro option requires a bit less work for the first two steps, as no...

  8. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ... Digital or print distribution.  Static. Often requires both artistic and scientific training. Cartoons...media type to another is cognitively demanding. Requires multiple areas of expertise.  Social Media ...scicomm. Difficult to do in-depth storytelling. Requires regular content production. Internet "trolls" ...

  9. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...statistical methods, omission of some data, etc.  The firestorm that followed implementation of the policy comprised...be accessed. Adding to this burden, the policy requires that data should be available for anonymous request...data authors. In some types of research, raw data requires huge amounts of storage. For example, mouse behavioral...

  10. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...conditional allele, or floxed mouse, from Yang et al. requires two gRNAs targeting two intronic regions and two...allele The design for a floxed conditional allele requires you to target 2 loxP sites to intronic regions...Overall, the main drawback of i-GONAD is that it requires a high-concentration, column-purified DNA template...

  11. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...incorporate new information into DNA. This also requires an exogenous DNA template. HDR, however, is a ...lightly! Indeed, truly achieving the gold standard requires not one but two rounds of single cell cloning ...limited to single nucleotide transitions but still requires a nearby PAM, although these are still early days...

  12. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...or protein level. The “correct” choice of tool requires careful balancing of the inherent advantages and...exogenous protein, successful induction of RNAi only requires the presence of dsRNA. In plants, CRISPR technologies...RNAi-based investigation. Creation of the RNAi trigger requires a few initial considerations, such as the starting...

  14. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...disease” often requires understanding the disease genetics and the “treatment” usually requires drugs. While...

  15. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...Being an entrepreneur is difficult; it requires an immense amount of self-knowledge and an unwavering...  To start, the average health science startup requires a great deal more capital to get off the ground...

  16. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ... The entire protocol takes about 2-3 weeks but requires less than eight hours worth of hands-on time. ...Dickinson et al. have developed a modular system that requires very little hands-on labor and could facilitate...

  17. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...capable of mutagenesis in E. Coli, but this approach requires special bacterial growth conditions in order to...Phage Assisted Continuous Evolution (PACE) method requires constant turbidity of the culture, so bacteria...

  18. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...provides a truly random digestion, but is harsher and requires more optimization. Enzymatic digestion, while ...https://www.wannapik.com/   Even though ChIP requires a larger upfront investment than many other antibody...

  19. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...though. Viral packaging and entry The genetic cargo requires viral packaging to infiltrate and integrate into...target different tissue or cell types. AAV also requires some back up from adenovirus genes – E4, E2a, ...
Showing: 41 - 60 of 253 results