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Showing: 41 - 60 of 151 results

  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...luciferase fragment (expressed separately) assemble and catalyze the luminescence reaction (Figure 5). The researchers...under tension. Upon binding, LgBiT and BiT Tag catalyze a luminescent reaction with the substrate furimazine...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...Flow cytometry is a method that allows users to analyze single cells in a population. It can be used to...many variations in how one can gate, select, and analyze data. But now that you can read a FACS plot and...

  5. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...crucial for us to keep pace with our current need to analyze nearly 300 sequences each week. Our internal software...sequence that would be ready for our QC team to analyze. Criteria for a bioinformatics workflow management...

  6. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...becomes active when illuminated with blue light, catalyzing the biotinylation of nearby proteins. B) TransitID...ticker tape from cFos::GFP-iPAK4 within an hour. Analyzing the ticker tape images could recover transcriptional...

  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...directed evolution (Tei et al., 2023).  PLD can hydrolyze phosphatidylcholine (PC), the predominant membrane...labeling via click chemistry.    Figure 4: SuperPLD hydrolyzes membrane PC to PA. A) OptoPLD is recruited to...

  8. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...Learn More about Restriction Cloning Get Tips on Analyzing DNA Sequencing Results Check out Plasmid Cloning...Our Protocol Pages Find Molecular Biology Tools Analyze Your Plasmids by Restriction Digest ...

  10. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...When analyzing your cells using flow cytometry, you are typically measuring the presence or absence of...possible. Finally, we designed a simple panel to analyze transduced HEK 293T cells. Here, we looked at both...

  11. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...specific antibody and a large number of cells to analyze. A new technique from Michael Rosbash’s lab, TRIBE... starting material! Thus, TRIBE can be used to analyze a particular cell population within a given tissue...

  12. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...needs. I hope you find the dashboard useful when analyzing and sharing your own depositor metrics!  About...

  14. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...We've got ~50 Addgenies working to ship plasmids, analyze QC sequence, answer scientists' questions, manage...

  17. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...on one aspect alone. They can study molecules, analyze terabytes of information on black screens, but ...

  19. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ... Substrate was then added and cleavage product analyzed on a gel. SpCas9 is stable when incubated in reaction...
Showing: 41 - 60 of 151 results