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We narrowed to 461 results for: MEA;

Showing: 41 - 60 of 461 results

  1. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...? The TRUPATH collection allows researchers to measure the physical association of combinations of different... signaling pathways.  This dissociation can be measured using a technique known as Bioluminescence Resonance...version of BRET called BRET2. Figure 1: TRUPATH measures heterotrimeric G protein dissociation by bioluminescence...decreased GFP2 emission. The activation state can be measured by a BRET2 ratio, which is the ratio of GFP2 emission...both antagonists and inverse-agonists could be measured using the TRUPATH system. Conclusions Although...

  2. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ... described, then measured electrophysiological potential afterwards. This measurement trended with expression...stimulate or manipulate responses through neurological means like electric stimulation, drug administration, ...fibers to emit light for optogenetic purposes, measure temperature, study electrophysiology, and deliver...Alternatively, the system could be used to deliver or measure new variables. The sky is really the limit for ...

  3. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ...PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article contributed by...ApoB bound lipoproteins (ApoB-LPs) are indirectly measured through their triglyceride or cholesterol contents...correlation with cardiovascular disease and indirect measurement of ApoB-LPs through triglyceride/cholesterol ...al, 2012; Lawler et al, 2017).  In an effort to measure ApoB-LPs directly and develop a potentially high... and offers many advantages over traditional measurements such as patch clamp recordings. However, technical...

  4. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...proteins are out of the measurable range of the dilution used in an assay, meaning that your normalization...you see a smiley face in your gel, or if you see smeared bands, your voltage is likely too high. If you’...   Ladders If you need very accurate protein measurements, consider using an unstained protein ladder,...conjugated antibody. If you do not need such precise measurements, you can use a colorimetric ladder, visible ...to adjust ‌your antibody dilution to accurately measure these proteins. That being said, normalizing to...dilutions are showing the same signal (Figure 3B), that means the amount of protein loaded is outside the detectable...

  5. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... this class measures either factors as proxies for a cellular process or directly measure a cellular process...boiled down pretty simply – they are systems that measure biological phenomena by generating an output which...microscope, plate reader, or any other instrument which measured GFP levels and converted that signal into a readable...high tension. These systems are powerful tools to measure real-time cellular reactions to stimuli and as ...

  6. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...(usually a laser in fluorescence assays), which means luminescence assays have lower background levels...luciferase gene, and expression of luciferase is measured via the light produced. A similar approach can...assays require cell lysis as the most efficient means to disrupt the cell membrane and deliver the substrate...luciferase or alternative substrates can permit measurements of luminescence from live cells. Luciferase ...with experiments that supply both substrates and measure luciferase output at different wavelengths. Addgene...

  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...fixation and permeabilization protocol, but very poorly in a methanol fixation-permeabilization protocol.... can also be affected by cell fixation and permeabilization techniques — and so it may be necessary to...mixed in a 1:1 ratio and fixed in 4% PFA and permeabilized in 0.1% saponin. 400,000 cells were stained ...also save money!); and optimizing fixation/permeabilization reagents for intracellular targets. Since...sample with mixed expression, with the expression measurable through an epitope tag such as FLAG/His/GFP. ...

  8. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...between 0.80-1.20) would mean you have one copy of the gene; an RQ value of 2 means you have two copies, ...triplicate.  The nice thing about qPCR is that since it measures relative quantification - that is, since you’re... a light technical control - an RQ value of 1.5 means you have either made a pipetting error or an incredible...comparison of assays for accurate copy number measurement of the low-affinity Fc gamma receptor genes FCGR3A...

  9. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ... antibodies may have similar emission spectra, meaning they will emit fluorescent light at similar wavelengths...record the fluorescence for both at similar levels, meaning that your populations will ‘look’ similar on your...two channels (Figure 1A). Applying compensation means you are telling your cytometer to adjust the fluorescent... does NOT change anything in your samples. This means that, unlike voltages, you can adjust compensation...compensate in the top right corner of a flow plot, meaning that it should be double-positive for the two markers...

  10. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...commonly used to measure relative quantification of mRNA, which can be used as a proxy measure for differences...primers. This separate thermocycle allows you to measure the intensity of signal over an entire cycle. Since...want to validate your primer/probe through another means, such as an agarose gel or uMelt analysis (Downey...verification. Primer dimers will often show up as a smear or band around 30-50 bp. uMelt analysis  uMelt is...previously, just because it’s used often doesn’t mean it’s right for your experiment! The model assumes...

  11. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...is the sum of the mean rate for each paper. Green and red lines depict the sum of mean +1 and -1 std for...Smoothing our plasmid order data with a 7-day rolling mean gave us a crude insight into the magnitude of the...of COVID-19 on data smoothed with a 7-day rolling mean. While this global approximation already shows... could be different.  Conclusion Figure 7: Mean inferred effects by region. In conclusion, our...

  12. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...single cells in a population. It can be used to measure protein expression, identify rare cells, or even... cytometers, and output is generated through measurement of events. An event is something that passes ...scatter (SSC) and forward scatter (FSC), which measure light that “scatters” past the cell, instead of... doublets and debris. The side scatter roughly measures granularity, a proxy for nucleic complexity of...it from left to right.  First, SSC and FSC are measured to differentiate cells from debris. Cells are ...

  13. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...and the distances the packages needed to travel meant the accessibility varied country to country.   Figure... you can imagine, shipping to so many countries means that our shipping team is always engaged in some...researchers all over the world — and we really do mean all over (Figure 3). We continue to add new posts...Stats and figures are helpful, but our favorite measure of our educational resources’ value is feedback...digital content. We have implemented proactive measures to find potential barriers that may not be obvious...

  14. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...inactivated native restriction/methylation system. This means the strain can neither restrict nor methylate DNA... proteins Tuner (DE3)   Contains mutated lac permease whch allows for linear control of expression ...most experiments, the lac promoter can “leak,” meaning that a low level of expression exists even without... these cases, some strains carry an additional measure of control such as the pLys plasmid, which suppresses...fear not—there are a few simple troubleshooting measures that can help get you back on track: Compatibility...

  15. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you should be happy to learn that...pSOUP to mCherry, satisfying ingredients reagents permeate the molecular biologist’s kitchen lab - and scientists...generated allows for qualitative and quantitative measurement of processes including RNA movement by tagging...hydroxybenzylidene imidazolinone (DFHBI). DFHBI is membrane permeable and non toxic to cells. Because it rapidly photobleaches...transcribed by RNA Polymerase III (5S, tRNA and U6) and measuring Corn fluorescence by flow cytometry, the Jaffrey...

  16. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...of everyone in government (19). To defend the meaning of the word ‘fact’. Policy must be based on our...research, more funding is required. This is by no means an exhaustive list and every marcher is not necessarily...For science to be integrated into politics in meaningful ways, continued conversation about science’s ...on your state in this map. Finally, the march is meant to inspire scientists to be more involved in their...Better Science Nature: What Trump’s new travel ban means for science Nature: Meet the scientists affected...

  17. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...about are (1) your experimental conditions and measurements; (2) the cells you’re targeting; (3) how much... et al., 2018; Korneyenkov & Zamyatnin, 2021), meaning what works great in one system or cell type can...need to test for this in your system if you’re measuring expression over a period of weeks (in vitro) or...cells you want to deliver to, and how you’ll be measuring your experimental output. You’ll also want to ..., at an adequate level for your experimental measurements. The number itself is determined through validation...

  18. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...high affinity for an endogenous molecular target, meaning the dye will be attached to the target through ...protein of interest, these tools provide an easier means to identify targets through fluorescent labeling...minimal background staining. Additionally, cell permeability is a critical factor, as it influences the dye's...light when excited. While it is technically cell-permeable, its use in live cells can sometimes result in...in cellular toxicity due to the permeation process. Advanced technologies in fluorescent dyes/ligands...

  19. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...familiar. The idea that my piecemeal degree might one day foster a meaningful day job only came into focus...interested in connecting with the public in a meaningful way, and not solely to ensure a profit. Often... of my thesis: a survey instrument designed to measure how individuals respond to issues of unsettled ...

  20. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...antibody is a monoclonal antibody. But what does that mean, and how do monoclonal antibodies differ from other...antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive this step. Then...then used to produce all lots of the antibody, meaning that the antibodies are largely identical between...antibody that is actually produced by the hybridoma, meaning that there will be changes between lots over time...
Showing: 41 - 60 of 461 results