We narrowed to 55 results for: MLS

...necessary. To save time and money, I like to miniprep 5 mL of my AAV transfer plasmid cultures and then screen...

...cassette, and illustrates how the volume of the 1 ml outgrowth spread on each transformation plate can...

...Guest Blogger, Jennifer Rivers! Jennifer Rivers ScM, MLS(ASCP)CM is a research microbiologist specializing...

...ultrasound contrast. In all the cell lines we tested, 1 µg/mL doxycycline was sufficient to saturate expression...

...Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G (2015) Trans-spliced Cas9 allows cleavage ...

...hard to detect with levels reaching 108 cells per ml before the media becomes cloudy. Mycoplasma compete...

...Garcia KF, Barnes KG, Chak B, Mondini A, Nogueira ML, Isern S, Michael SF, Lorenzana I, Yozwiak NL, MacInnis...

...plasmids from this publication at Addgene. Maeder ML, Angstman JF, Richardson ME, Linder SJ, Cascio VM...

...For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in liquid nitrogen or a dry ice...

...: 18607781. Schleef M, Baier R, Walther W, Michel ML, Schmeer M. Long-term stability study and topology...

...were transduced (MOI = 37) and selected with 1 µg/mL blasticidin for 9 days. Single cells from a stable...

...https://doi.org/10.1534/genetics.114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors for introducing...

...plasmids can be submitted as 15 µL of DNA in a 1.5 mL microfuge tube at a concentration of 0.1–1 µg/µL ...

...pTRE-Tight-MitoTimer and rtTA. The cells were pulsed with 2µg/ml of Doxycycline for 2 hours, and imaged on the Keyence...

...animal (i.e., 10 and 50 µL of vector at 1 x 1013 vg/mL titer), while AAV-PHP.S is typically used at a dose...