We narrowed to 215 results for: Mac
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TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....resulting in processing by the endogenous RNAi machinery. Alternatively, endogenous miRNA precursors can...initial state of the dsRNA upon activating the RNAi machinery (long dsRNA, typically hairpin RNA, or artificial...-2005-837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based ...
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Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....2. Fluorescence Activated Cell Sorter (FACs) A machine that sorts cells according to whether or not they... deflect them into appropriate containers. The machine is useful for distinguishing cell populations that...PMCID: PMC2569002. 3. Egen, Jackson G., et al. "Macrophage and T cell dynamics during the development and... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog PostPublishedMarch 25, 2025, 1:15 p.m....were included in the CGBE. They also developed a machine learning model capable of predicting editing outcomes... CRISPRi screens, target-library analysis, and machine learning. Nature Biotechnology, 39(11), 1414–1425...with sequence context preference predictable by machine learning methods. Nature Communications, 12(1),... -
#Deckthelab Contest Winners 2017
TypeBlog PostPublishedJan. 31, 2018, 2:44 p.m....crafty holiday decor that we will never forget! The Mackin Lab from University of Colorado -Anschutz Medical...cheer, and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis (@AlexDainis... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog PostPublishedMarch 7, 2019, 2:39 p.m....contamination, but consider flow cytometers. These fluidic machines handle biological samples and are cleaned between...between each use. You wouldn’t throw away your FACS machine after a single use. So how about washing and reusing... -
Science as Art
TypeBlog PostPublishedMarch 2, 2015, 3:13 p.m....non-academic people.” Daniel went on to discuss his work: “Macro photography has been my passion for a while - a ...teacher of dental radiography, had focused his x-ray machine not on teeth, but on blossoms, thus revealing the... -
Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog PostPublishedOct. 18, 2017, 1 p.m....Department of Pharmacology, I taught a couple of lectures in the undergraduate pharmacology course, like... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog PostPublishedSept. 15, 2016, 2:30 p.m....protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. When I joined Sanofi/Genzyme...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. His work experience is in... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they enter a cell...delivered to the cell. The cell then uses its own machinery to replicate the viral genome and proteins. This... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....studies showed that iTOP functions through macropinocytosis. D’Astolfo et al. tested iTOP in a series...Protein Delivery Techniques: Introduction of macromolecules into cultured mammalian cells by osmotic lysis... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog PostPublishedApril 14, 2016, 2:30 p.m.... our purposes? In search of simple biological machines R bodies can repeatedly extend and contract...using R bodies: simple, force-generating protein machines that can extend and retract over many microns ... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m.... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....recombineering, bacteria express phage recombination machinery, which facilitates easy genetic modification by...electrocompetent cells in which the phage recombination machinery is induced using L-arabinose. These cells can ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing to...plants. Yang was intrigued by the tRNA processing machinery present in almost every living organism. Precursor... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog PostPublishedApril 13, 2017, 2:30 p.m....unless autophagy is induced. Then non-selective macroautophagy of the cytosol leads to accumulation of Pho8Δ60.... E. (2015). Rapid parallel measurements of macroautophagy and mitophagy in mammalian cells using a single... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog PostPublishedSept. 8, 2020, 2 p.m....that trigger the activation of dendritic cells, macrophages, T cells, and the recruitment of neutrophils....understand so that we could possibly develop pharmaceutical interventions that would inhibit IL-36 release... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog PostPublishedJune 26, 2018, 1:33 p.m....proteins assemble into functional multi-subunit macromolecular complexes, but a serendipitous discovery led... amino acid. Clearly, the development of a pharmacological intervention that rescues the function of even... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....Guided by dCas9, VP64 recruits transcriptional machinery to specific sequences, causing targeted gene regulation...of interest, this allows more transcriptional machinery to be recruited per targeted gene. Performance... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m.... activity. They interact with transcriptional machinery to modify local gene expression, confounding any...genome and directly interacting with transcription machinery. Well, I’ve got some good news: complete... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m....were active in mouse retinas were injected into macaques and postmortem human retinas. About 25% of these... library for retinal cell expression in mouse, macaque, and man. Lab Anim 48, 264 (2019). https://doi....