We narrowed to 184 results for: SIR

... Antibiotic selection would only then kill the desired plants! PCR or fluorescent markers are currently...from these plants will be green, containing the desired mutation but lacking both RUBY and the construct...

...retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned into the vector...weeks, depending on the virus, cell type, and/or desired event.   Figure 1: Transduction to expression...

... my curiosity did not ultimately translate to a desire to do the research itself. Several years of exclusively...more creative faculties into motion, such that a desire to once again write for fun eventually saw an English...

...says that the lab got interested in XP out of a desire to understand the early events in the transformation...syndrome (WS4) by Pediatrician Duangrurdee Wattanasirichaigoon carried different mutations in a gene called...

...if you end up concentrating your AAV above your desired titer and want to dilute it, err on the side of...less buffer than you would to achieve the exact desired concentration to avoid over-diluting, and make ...

...that mismatch repair (MMR) interfered with the desired resolution of prime editing intermediates. Building...sequence. As with other prime editing tools, the desired edits are templated by the 3′ extension of the ...

...is tested following each immunization until the desired level of antibodies is reached - typically within...within 2-4 months. After the animal has the desired level of antibodies in its bloodstream, the antibodies...

...all the diverse in vitro production mechanisms. siRNA, also known as RNAi, are short, dsRNA molecules ...therapeutic delivery. Thus, balancing the amount of siRNA to be delivered or anticipating this response are...

...photobleach at some point. For live-cell imaging, it is desirable to have fluorescent proteins that are photostable...photoswitching (Kremers et al, 2009), which usually are undesired properties. In the ideal situation, a fluorescent...

...of ccdB, leading to positive identification of desired clones.   ...Becomes a powerful cloning tool... efficiently, as those that do not contain the desired insert should not grow.   Figure 2: Image...

...new job skill? Finish your thesis? Knowing your desired outcomes will help you assess if the coach you ...relationship has been successful – what are the desired outcomes? Ask openly for clear information on...

...gene silencing mechanism. Scientists can express siRNAs to direct the cellular RNAi machinery to silence...the Weinberg lab enables researchers to express siRNAs of their choice in mammalian cells.  Learn more...

...transcription of the template and initiating the desired cleavage event, although some engineered terminators...downstream elements. A high affinity terminator may be desired for multi-cistronic constructs where high termination...

...to introduce yourself to a few members of your desired department during the application process. Many...However, an online meeting with members of your desired program will allow for you to have the time and...

...restriction enzymes. In Golden Gate cloning, the desired end construct does not contain the type IIS recognition...This feature biases the reaction in favor of the desired assembly to make the process close to 100% efficient...

... Inducible promoters are extremely useful if a desired protein product is toxic to cells at high concentrations...allows for easier access and purification of a desired protein. Despite being a rather nascent technology...

...(Are the inserts of different sizes?), and the desired coverage for the library (How important is it that...  The most important factor to consider is your desired library coverage, since this will help decide what...

...primary method to produce the linear fragments, as desired modifications and homologous regions can be encoded...order primers that bind at either side of the undesired portion, designed to amplify the rest of the vector...

...: Clone spacer(s) into the CRISPR array Clone desired DNA cargo into the mini-transposon Transform plasmid...even very large genetic cargo, at one or multiple desired genomic loci. Many applications of these systems...

...updated Aug 20, 2020. Cas9 can be used to modify any desired genomic target provided that (1) the sequence is... may be unintentionally mutated along with the desired target locus. The PAM sequence is of particular...