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We narrowed to 186 results for: SPL;

Showing: 41 - 60 of 186 results

  1. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...(AAV). The components of these vectors are also split across several plasmids (often three), for safety...virus must assemble and have appropriate factors displayed on the outside of the particle to interact with...components needed for viral packaging, assembly, and display. Pol, the last component, contains the reverse ...

  2. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...constructing split variants should be relatively straightforward, but it is also possible to create split versions...modular protein, Tang et. al were able to create a split version of Cre that is only active when dimerized...

  4. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...talking to themselves as they compare the features displayed on the model with their mental model of the protein...primed for cleavage. This structure captures the displaced non-target DNA strand and shows the HNH nuclease...target DNA strand. In addition, this model also displays the several Lys/Ala mutations reported by Ian ...

  5. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...each sample. Successfully analyzed samples will display the following parameters: Sample - The unique ...analysis can be sorted by any of the parameters displayed in the summary table. You can also use your browser..., you’ll find an Indel plot (left panel) which displays the inferred distribution of indel sizes in the...

  6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...sequence NGG compared to wild-type SpCas9, which also displays some cleavage with an NGA PAM. This variant may... AAV delivery. NmCas9, another small ortholog, displays lower off-target editing than wild-type SpCas9...rather than the blunt ends created by Cas9. Cas12a  displays lower off-target editing than SpCas9 (Kim et al...

  7. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...developed into a split aptamer that can be used to investigate RNA assembly. The split aptamer is composed...References Alam, Khalid K., et al. "A fluorescent split aptamer for visualizing RNA–RNA assembly in vivo...

  8. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...for DNA repair synthesis. The ssDNA strand then displaces one strand of the homologous DNA donor and can...formation of a DNA structure referred to as the displacement loop (D loop). The recombination intermediates...synthesized portion of the invasive strand is displaced from the template and returned to the processed...

  9. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...abundant and ethanol concentrations are low, pADH1 displays low promoter activity (~20% activity of the strong...complexity of the system, researchers have used split GAL4 architecture or combined the system with Flp...Valle Rodriguez, Alberto, Dominic Didiano & Claude Desplan. “Power tools for gene expression and clonal analysis...

  10. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...negative effect on these parameters. IT5 and IT6 displayed good binding efficiency in both immunofluorescence...cyclin Cdc28 and the chaperone Ydj1, both of which display sophisticated interactions with multiple other ...

  11. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...Jurassic Technology in LA, where it is currently on display.   Many universities are now following the trend...scientific art competitions where creations can go on display for all to enjoy. The largest competition of this...

  14. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...small (Rep52 and Rep40) Rep proteins. Alternative splicing generates the different C-terminal regions of ...often driven by the AAV p40 promoter. Alternative splicing and differing translational start sites generate...

  15. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...movement. When the ribosome moves along an mRNA, it displaces other RNA-binding proteins to allow translation...translation to occur. For a TRICK reporter mRNA this displacement leads to a change in the fluorescent signal:...

  16. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...cannot be deleted, so there is no worry about misplacing your results or losing that lab notebook from...similar apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega...

  17. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...Interactions with Split-BioID Find resources for studying chromatin Resources on Addgene.org Find Split-BioID plasmids...

  19. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...meant for isolating RNA from rat spleens in which they homogenized spleens in a guanidinium thiocyanate solution...

  20. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...generated a photosensitive Cre, Opto-Cre-Vvd, by splitting and joining the protein regions with Vivid (Vvd...low and high-intensity blue light. Unlike other split-Cre systems which can take up to 24 hours to cut...
Showing: 41 - 60 of 186 results