...University of Wellington, Victoria, has developed an adaptable bacterial expression vector which gives close ...and C-terminal tags. Additionally, by using an adapted destination vector with a heterologous promoter...activation plasmids have great potential and have been adapted for use in mammalian cells, yeast, and Drosophila...during their transport. Finally, the system can be adapted to cellular screening to identify molecules that... overlap with the optogenetic actuator. In 2012, Adam Cohen's lab presented Archaerhodopsin 3 (Arch) as...a tool for monitoring multi-gene expression and adapt it for multi-color calcium sensing (plasmids coming...

...originally utilized CRISPR/Cas as a powerful and adaptive defense strategy against life-threatening viruses...scientists to hypothesize that prokaryotes developed an adaptive immune system that utilized various cas genes ... of anti-CRISPR genes in phages The CRISPR/Cas adaptive immune system seems like the winning ticket to...

...including organelles, are targeted to lysosomes for degradation. Autophagy is a dynamic process which involves...materials to be degraded to the lysosome, and degradation of autophagic substrates inside the lysosome....autophagasomal membranes, which leads to its degradation by autophagy. The RFP-LC3∆G, however, can not...

...University of Ibadan, Nigeriaunder supervision of Dr. Akindele Oluwatosin Adeyi, University of Ibadan, Nigeria... Akindele Oluwatosin Adeyi at the University of Ibadan. She works with medicinal plants such as Launaea...

...their devices, which makes it very difficult to adapt the device to one's own samples and limits the use...to as 'frugal science', existing components are adapted and combined with creativity and feedback from ..., for example, can also be printed quickly in Madagascar. Platforms that allow for on-going creativity...

...structure. I quickly learned that my approach needed to adapt based on individual needs. For instance, I had one...projects autonomously. Failing to recognize and adapt to these differences could have led to frustration...experienced this firsthand when it took me some time to adapt to the communication style of a new, junior team...

...groups are susceptible to hydrolysis and heat degradation. Furthermore, RNases, or enzymes that degrade...samples cold and work quickly to mitigate RNA degradation. Make sure to use DEPC-treated or RNAse free ...store them in a -80°C freezer to prevent RNA degradation. They will degrade in a -20°C freezer.   RNA...

...features and evolved PAM sites, making Cas9 an adaptable choice for many genome engineering endeavors. ...target sequence and initiates single stranded DNA degradation. Cas3 DNA digestion can proceed up to multiple...   Choosing a Cas While Cas9 may be the most adaptable Cas currently available, the other Cas proteins...

..., gRNAs can be chemically modified to resist degradation while still effectively serving their function...recognized as foreign RNA. To protect the RNA from degradation, several simple methods have been employed which...stability.  Nature has evolved ways to prevent degradation of important cellular RNA molecules with post...

...packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA sequences to instead drive...delivering gene editing tools into cells. Image adapted from Segel et al. 2021.   Prime editing improvements...engineered pegRNAs (epegRNAs) protect the RNA from degradation by cellular exonucleases and broadly improve ...

...only infect Cre-positive, TVA-expressing cells (Osakada et al., 2013). Ultimately, this allows RABV to ...Pubmed PMID 23487772. 3. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread...Neuroanat. 2015 Jul 1;9:80. Pubmed PMID 26190977. 6. Osakada F, Callaway EM. Design and generation of recombinant...

...Executive Director changed twice. I had to learn how to adapt to different situations and, in particular, to different...predecessors. Changes were implemented and I had to adapt accordingly. For example, the quarterly and annual... would come necessary changes. I also learnt to adapt my leadership style according to the needs of my...

...section below.) For guide design, we decided to adapt the popular guide design tool Crispor by Max Haeussler...Crispresso CrispRVariants AmpliCan We chose to adapt Crispresso by Luca Pinello and Kendell Clement for...completed a MSc in Psychology at McMaster University in Canada. He's happy to be contributing again to science...

...method for genomic insertion. PITCh can also be adapted for whole-plasmid integration if you’d like to ...from a given promoter, and the technique can be adapted to other transgenes. It’s important to note that...increased versatility, it would be advantageous to adapt PITCh to insert genes into AAVS1, the “safe harbor...

... Lia Petronio, Katherine Huang, Alham Saadat, Thomas Green, Adam Brown, John G Doench, David E Root, James...worked with John Doench, David Root, Tom Green, and Adam Brown from Broad's Genetic Perturbation Platform...

...folding, post-translational modification, protein degradation, access to the nucleus in eukaryotic screens ... system has been devised (8). References 1. Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J....Stynen B., Van Dijck P., Tournu H. A CUG codon adapted two-hybrid system for the pathogenic fungus Candida...

...represent a functional CRISPR nuclease that could be adapted for genome editing. The use of a different nuclease... the simplest CRISPR systems and the easiest to adapt to genome editing, but the introduction of type ...learn about CRISPR biology. Later work, like the adaptation of Cas13 to RNA targeting and RNA editing, has...

...domain of RNA deaminase ADAR2. Constructs containing the ADAR2 truncation ADAR2DD(delta984-1090) are approximately...

...applications. The first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1...targeting flexibility. Cas13a (formerly C2c2) has been adapted for targeted RNA cleavage in mammalian cells. The...Replacement) system fuses Cas13b to RNA deaminase ADAR2 to create a specific RNA editor. Cas13 enzymes are...

...proteins, and modifications to the pegRNA. The Britt Adamson Lab recently used a CRISPRi screen to identify ...efficiencies at different loci in U2OS cells. Image adapted from Yan et al. 2024 under a CC-BY license. ...transduction efficiency for the indicated ssAAV5s. Figure adapted from Serrano et al. 2023 under a CC-BY-NC-ND license...