...scientists are paid above this threshold or for tracking the hours that these scientists work and paying...across academia). Increased postdoc wages, overtime tracking? What are institutions doing? Institutions are... the University of Colorado are doing, and are checking with Yale that they are raising salaries as their...

...cargo Optogenetic proximity labeling and TransitID Tracking cellular history with protein ticker tape Teaching..., et al. (2023). Dynamic mapping of proteome trafficking within and between living cells by TransitID.... bioRxiv – doi: 10.1101/2023.02.07.527548.    Tracking cellular history with protein ticker tape By Brian...

... you’re an oldbe trainee!) can be pretty nerve-wracking. So how can you be the best trainee possible? ...group more (at least at first) than any other one. Luckily, you can do a lot to contribute to the relationship...tell a trainee is understanding and things are ’clicking’ for them. It’s often easiest to train individuals...

...do. A popular screening method for knockouts is Tracking of Indels by Decomposition (TIDE) (Brinkman et... software very similar to TIDE known as TIDER (Tracking of insertions, deletions, and recombination events... you might expect off target editing to occur. Luckily, in silico prediction analyses like CRISPRitz or...

...directly trafficked to cell membranes. Membrane trafficking of farnesylated derivatives enables labeling ...fluorescence due to the addition of translation-blocking STOP cassette immediately following the promoter...dev.094631. PubMed. Multiplex cell and lineage tracking with combinatorial labels. Loulier K, Barry R,...

...sticky” proteins expressed by your library. After blocking for 1 h at room temperature with gentle shaking...harvested dose of cells (preferably in log phase) in blocking solution (around 108 CFU/mL is a good place to...onto nitrocellulose and carry out the steps from blocking the membrane to probing with cells in order to...

...pro’s and con’s of working in different sectors. Luckily, I have worked for a variety of different organizations...and problem solving. I believe that is something lacking during degree courses; they never really prepare...the costs. My MSc courses were interesting and luckily genetics is a sector which has been rapidly growing...

...protein-peptide locking, Mark Howarth’s lab has developed a new tool for peptide-peptide locking. The new technology...Easy-to-use tools to monitor these features were lacking until Roberta Gottlieb and Zhen Yan labs developed...mitochondrial membrane. Sequestration is accomplished by knocking down your endogenous protein of interest and co-expressing...

...hardware sold by the manufacturer. Blocking solutions Your blocking options come in two categories: protein-based...information. Many antibodies will have a recommended blocking buffer and a recommended concentration. That makes...

...Anterograde transport is typically used for the trafficking of organelles, such as mitochondria, as well ... The underlying mechanisms of trans-synaptic trafficking are not fully understood and may be a form of...B.T., Maguire, C.A. and Ramirez S.H. (2017). Trafficking of adenoassociated virus vectors across a model...

...or not know where to start looking to find one. Luckily, there are websites that can help: Antibody data...antibody quality throughout research, consider checking out the Only Good Antibodies community on LinkedIn...

... you leverage your community, think hard about picking a good mentor, and begin considering career options...about them? 18:30 - 19:54 : How to think about picking a post-doc project 19:55 - 22:40 : Improvements...

...protein tracking in vivo. Sci Rep. 2015 Jul 7;5:11771. doi: 10.1038/srep11771. PubMed. McKinney SA, Murphy...

...write brief descriptions on each. Place the notes sticking up out of the top of the notebooks, so that you... knowing the information is backed up and edit tracking will help you identify when others have added ...

...contacting someone else and waiting for an answer. Luckily, there’s a trend in customer support to address...icon, search for answers, and choose a result. By clicking on the result links themselves right from within...

...Streptococcus and the “py” in pyogenes), capable of locking two peptide tags together. SpyLigase overcomes ..., highly stable and has this amazing chemistry locking the domain together - no one knows what for. Many...

...communication a non-negotiable part of your collaboration. Luckily, there are techniques and tools that helped make...accountable to these expectations once they’re set by checking in about them at your recurring meetings and reaching...

...fluorescent signal and enables single molecule tracking  within living cells without affecting protein...photobleaching Simplified, long-term single-molecule tracking Caveats of SunTag Very large size: a 24x scaffold...

.... These unrelated ideas might hold the key to unlocking new perspectives or even revolutionizing your ... the unexpected — it might just be the key to unlocking the next frontier of knowledge. Matteo Tardelli...

... be green, containing the desired mutation but lacking both RUBY and the construct that rescues the mutation...RUBY was integrated into a CRISPR system, then tracking the segregation of the CRISPR construct could ...