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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 283 results for: cki

Showing: 41 - 60 of 283 results

  1. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...cargo Optogenetic proximity labeling and TransitID Tracking cellular history with protein ticker tape Teaching..., et al. (2023). Dynamic mapping of proteome trafficking within and between living cells by TransitID.... bioRxiv – doi: 10.1101/2023.02.07.527548.    Tracking cellular history with protein ticker tape By Brian...

  2. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ... you’re an oldbe trainee!) can be pretty nerve-wracking. So how can you be the best trainee possible? ...group more (at least at first) than any other one. Luckily, you can do a lot to contribute to the relationship...tell a trainee is understanding and things are ’clicking’ for them. It’s often easiest to train individuals...

  3. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...scientists are paid above this threshold or for tracking the hours that these scientists work and paying...across academia). Increased postdoc wages, overtime tracking? What are institutions doing? Institutions are... the University of Colorado are doing, and are checking with Yale that they are raising salaries as their...

  4. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...do. A popular screening method for knockouts is Tracking of Indels by Decomposition (TIDE) (Brinkman et... software very similar to TIDE known as TIDER (Tracking of insertions, deletions, and recombination events... you might expect off target editing to occur. Luckily, in silico prediction analyses like CRISPRitz or...

  5. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...directly trafficked to cell membranes. Membrane trafficking of farnesylated derivatives enables labeling ...fluorescence due to the addition of translation-blocking STOP cassette immediately following the promoter...dev.094631. PubMed. Multiplex cell and lineage tracking with combinatorial labels. Loulier K, Barry R,...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...protein-peptide locking, Mark Howarth’s lab has developed a new tool for peptide-peptide locking. The new technology...Easy-to-use tools to monitor these features were lacking until Roberta Gottlieb and Zhen Yan labs developed...mitochondrial membrane. Sequestration is accomplished by knocking down your endogenous protein of interest and co-expressing...

  7. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...sticky” proteins expressed by your library. After blocking for 1 h at room temperature with gentle shaking...harvested dose of cells (preferably in log phase) in blocking solution (around 108 CFU/mL is a good place to...onto nitrocellulose and carry out the steps from blocking the membrane to probing with cells in order to...

  8. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...pro’s and con’s of working in different sectors. Luckily, I have worked for a variety of different organizations...and problem solving. I believe that is something lacking during degree courses; they never really prepare...the costs. My MSc courses were interesting and luckily genetics is a sector which has been rapidly growing...

  9. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...hardware sold by the manufacturer. Blocking solutions Your blocking options come in two categories: protein-based...information. Many antibodies will have a recommended blocking buffer and a recommended concentration. That makes...

  10. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...Anterograde transport is typically used for the trafficking of organelles, such as mitochondria, as well ... The underlying mechanisms of trans-synaptic trafficking are not fully understood and may be a form of...B.T., Maguire, C.A. and Ramirez S.H. (2017). Trafficking of adenoassociated virus vectors across a model...

  11. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ...or not know where to start looking to find one. Luckily, there are websites that can help: Antibody data...antibody quality throughout research, consider checking out the Only Good Antibodies community on LinkedIn...

  12. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ... you leverage your community, think hard about picking a good mentor, and begin considering career options...about them? 18:30 - 19:54 : How to think about picking a post-doc project 19:55 - 22:40 : Improvements...

  13. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...protein tracking in vivo. Sci Rep. 2015 Jul 7;5:11771. doi: 10.1038/srep11771. PubMed. McKinney SA, Murphy...

  14. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...write brief descriptions on each. Place the notes sticking up out of the top of the notebooks, so that you... knowing the information is backed up and edit tracking will help you identify when others have added ...

  15. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...contacting someone else and waiting for an answer. Luckily, there’s a trend in customer support to address...icon, search for answers, and choose a result. By clicking on the result links themselves right from within...

  17. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...communication a non-negotiable part of your collaboration. Luckily, there are techniques and tools that helped make...accountable to these expectations once they’re set by checking in about them at your recurring meetings and reaching...

  18. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...fluorescent signal and enables single molecule tracking  within living cells without affecting protein...photobleaching Simplified, long-term single-molecule tracking Caveats of SunTag Very large size: a 24x scaffold...

  19. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    .... These unrelated ideas might hold the key to unlocking new perspectives or even revolutionizing your ... the unexpected — it might just be the key to unlocking the next frontier of knowledge. Matteo Tardelli...

  20. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ... be green, containing the desired mutation but lacking both RUBY and the construct that rescues the mutation...RUBY was integrated into a CRISPR system, then tracking the segregation of the CRISPR construct could ...
Showing: 41 - 60 of 283 results