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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 256 results for: idua

Showing: 41 - 60 of 256 results

  2. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...web-based, career-planning platform called the Individual Development Plan (IDP) and uses it to match qualified...interests: Use career planning tools such as the Individual Development Plan to generate a list of potential...

  3. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...titers Making contact with a cell Although an individual AAV particle’s ability to infect a cell is dependent...high titers, as contact between the cell and any individual AAV particle is rare. But simply getting into...

  4. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...expression of the array. After expression, the individual gRNAs are released by endogenous tRNA processing...which is followed by tRNA processing to release individual gRNAs for gene editing. They named this new array...

  5. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...ends. This homology can be contained within the individual fragments themselves, but doesn’t necessarily... challenges can be circumvented by generating individual fragments containing mutations and aligned via...

  7. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...you ask? Well, organizations used to negotiate individual MTAs every time they wanted to share materials...Addgene — basically, instead of having to sign individual MTAs for each request, the depositing organization...

  8. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...DNA is harvested, much like one would prep an individual plasmid. However, lots of library DNA is needed...obtain about 100 to 1,000 bacterial colonies per individual plasmid in the library. Bacterial growth must...

  9. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ... left the arduous task of virus production to individual labs, but now we’re very excited to provide ready-to-use...uses nearly 80,000 gRNAs to target around 20,000 individual genes. We provide enough lentivirus for you to...

  10. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ... Cas9 endonuclease or a variant thereof. This individual protein has all the components necessary to: ...foreign DNA target sequences specific to each individual crRNA. Bottom line, the “direct repeat region...

  11. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...in real time PCR, each reaction partition is individually assessed for a positive or negative (digital...resolution of digital PCR allows classification of individual target molecules based on their mutation status...

  12. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ... rather than the day-to-day details. Ignoring individual differencesManaging a team isn’t “one size fits...learned that my approach needed to adapt based on individual needs. For instance, I had one colleague who ...

  13. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...everything else dies off. For a screen, you would individually treat cells with many different drugs and then... forward screens. A given panel of drugs are individually administered and then outcomes are measured....

  16. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...established, it’s also a process that’s difficult for individual labs to complete. The nanobody based RANbody ...purification. Antibodies are difficult for an individual lab to produce - Hybridomas offer an alternative...

  17. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...method it is not possible to follow turnover of individual proteins in situ.  For the visualization of specific...Söderberg, Ola, et al. "Direct observation of individual endogenous protein complexes in situ by proximity...

  19. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...pregnancy, the researchers were able to examine each individual blastomere to determine its genotype. However...regulatory and ethics committees comprised of individuals with varied backgrounds. Talking about this study...

  20. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...Gilbert et al., 2014; Konermann et al., 2014) individual genes for studies interrogating their functions...incredibly useful for the efficient generation of individual (and multiple) genetic perturbations in single...
Showing: 41 - 60 of 256 results