We narrowed to 62 results for: kan

1. Plasmids 101: Multicistronic Vectors

   Type

   Blog Post

   Published

   Sept. 9, 2014, 8:20 p.m.

   ...Lentiviral AmCyan-P2A-mCherry P2A Mammalian pC5Kan-P2A P2A Insect pUltra P2A and T2A Lentiviral ...

2. Tips for arabidopsis transformation

   Type

   Blog Post

   Published

   Oct. 25, 2018, 1:23 p.m.

   ... plant selection markers (basta, hygromycin, kananamycin, tunicamycin) (Nakagawa et al., 2007). Alternatively...

3. MXS Chaining

   Type

   Blog Post

   Published

   Feb. 7, 2017, 3:30 p.m.

   ...PubMed Central PMCID: PMC2822740. 2. Engler C, Kandzia R, Marillonnet S. A one pot, one step, precision...

4. Tips from the Repository Trenches: Using Barcodes to Track Samples

   Type

   Blog Post

   Published

   Oct. 6, 2015, 2:30 p.m.

   ...majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along the way involves...

5. Neuronal labeling with Spaghetti Monster

   Type

   Blog Post

   Published

   Aug. 14, 2018, 12:42 p.m.

   ...design in public schools and was started in 2005 in Kansas by Bobby Henderson, a physics graduate from Oregon...

6. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

   Type

   Blog Post

   Published

   July 29, 2025, 1:15 p.m.

   ...resistance gene as the plant-selectable marker and a kanamycin resistance gene for bacterial selection. The process...

7. Plasmids 101: Shuttle Vectors

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   Blog Post

   Published

   May 20, 2025, 1:15 p.m.

   ...aminoglycosides work on different host species: kanamycin targets the prokaryotic ribosome, while G418 (...

8. Hot Plasmids: Fall 2024

   Type

   Blog Post

   Published

   Nov. 21, 2024, 1:30 p.m.

   ...tetracycline repressor TetR and resistance selection marker KanR. This strain is then transformed with the sgRNA ...

9. Plasmids 101: E. coli Strains for Protein Expression

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   Blog Post

   Published

   Feb. 10, 2015, 3:06 p.m.

   ... General protein expression  m15 pREP4*, ** Kanamycin (pREP4) Cis-repression of the E. coli T5 promoter...

10. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ..., Rivera RM, Madhavan S, Pan X, Ran FA, Yan WX, Asokan A, Zhang F, Duan D, Gersbach CA. In vivo genome...

11. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    Published

    Sept. 1, 2015, 2:30 p.m.

    ... institute is located. For example, salaries in Kansas versus salaries in NYC will vary widely. Therefore...

12. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...proteins in the nervous system. Livet J, Weissman TA, Kang H, Draft RW, Bennis RA, Sanes JR, Lichtman JW. Nature...

13. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ...://doi.org/10.13070/mm.en.9.2846 Radtke, A. J., Kandov, E., Lowekamp, B., Speranza, E., Chu, C. J., Gola...

14. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    Published

    Nov. 2, 2021, 1:15 p.m.

    ...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020...

15. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...1510 . https://doi.org/10.3390/cells8121510 Sung H, Kang SH, Bae YJ, Hong JT, Chung YB, Lee CK, Song S (2006...

16. Antibodies 101: Antibody Engineering and Directed Evolution

    Type

    Blog Post

    Published

    Aug. 5, 2025, 1:15 p.m.

    ...23(9), 1126–1136. https://doi.org/10.1038/nbt1142 Kang, B. H., Lax, B. M., & Wittrup, K. D. (2022). Yeast...

17. Typing CRISPR Systems

    Type

    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ...https://doi.org/10.1038/nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K. S., Demircioglu...

18. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...25578634. PubMed Central PMCID: PMC4412587. 28. Mohsen-Kanson, T., et al., Differentiation of human induced pluripotent...

19. CRISPR 101: Multiplex Expression of gRNAs

    Type

    Blog Post

    Published

    Sept. 10, 2020, 11:45 a.m.

    ... ideal number. First you generate four unique kanamycin-resistant plasmids, each containing a different...

20. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    Published

    April 17, 2018, 1:37 p.m.

    ...Masayuki, Minoru Iijima, Manami Ohtaka, and Mahito Nakanishi. "Novel Strategy to Control Transgene Expression...