...are ways you can hone important skills for the profession. One of the most important skill sets for a science... of the who, what, where, when, why and most importantly, how, of a science policy career.  Who does science... place to start. What is science policy? THE IMPOSSIBLE QUESTION. Science policy is the work you do to...science policy? I would argue that this is the most important question that you can explore. The answer will...advice for you and your next steps. This advice is important, but must be taken through your own personal lens... science policy fellowship program which is a temporary position- anywhere from a few months to a few ...

...AAV plasmids contain several important elements, but among the most important are the inverted terminal ...Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene...modifications and types  Since ITRs are one of the most important elements of AAV and rAAV vectors, modifications...site reduce Rep nicking. Since Rep proteins are important for viral genome replication, these mutations ...is still limited, but this area of research is important for downstream AAV applications.  Tips & FAQs ...

...controlling cell signaling. Key components of OptoNBs OptoNBs have two key components: an AsLOV2 domain and a ...activity in cells.  Let’s shed some light on the key components of OptoNBs, how they are generated, and their...variable heavy chain antibody domain.  How do these components interact? When fused together to form an OptoNB...binding ability of its nanobody partner, so it’s important to insert AsLOV2 at a spot in the nanobody that...light to control signaling provides greater spatiotemporal resolution than pharmacological or genetic ...

...of an important surface protein (e.g. PD-L1 on a cancer cell). In this screen, known components of the...number of cells needed is lower, and perhaps most important, targeting all genes doesn’t necessarily add value...used these knockout mice to show exactly which components of the known pathway that were essential for ...response to a particular ligand (such as when a component of a microbe or a cytokine is added to a cell)... rapidly performed by targeting all potential components of a signaling pathway. For example, one could...are migratory, and their exact localization is important for their function. Their migration is induced...

...Tumors are composed of a wide variety of cell types. This cell heterogeneity is an important element of...still many important questions in this exciting field of research. One of the most important: How will ...commute to and invade multiple organs, and most importantly escape the immune mechanisms of the host. The...therapies themselves can be made more effective. Some important factors researchers are beginning to focus on ...successful tumor progression. A tumor’s genetic composition is constantly in flux and is dependent on the...

...for science, and numerous other reasons. It is important to remember that we, scientists and non-scientists...and calling for billions of dollars in cuts to important research funding.  So when I saw that there was...scientist.  I care about my work because it's important to our health.  Most people won't argue with that...with friends, family, and the public about its importance.  I think many of us grew to assume that the ...find it difficult to convey the excitement and importance of our research to family and friends outside...community members. The March for Science was an important first step towards starting these conversations...opportunity to cement in my children's heads the importance of science and positively reinforce their scientific...

...want to turn things on or off, permanently or temporarily, we’ve got you covered. Defining your control...requirements To pick the best tool for the job, it’s important to know what the job requirements are. Here are...inducible? How long do you need control? Will temporary control (a few days) be enough, or do you want...than making a knockout and is a great way to temporarily reduce expression of essential proteins (when...expression (more on this below) will both achieve temporary repression of protein expression. Addgene has ...via a custom gRNA, to your gene of interest to temporarily increase expression. There are several flavors...

...materials needed for optogenetics. There are several important considerations with fabrication of the fiber optics...your stimulation. The scatter effect is also an important consideration when preparing the implant itself...inactivation inconsistent, and behavioral results impossible to interpret. Polishing the fiber optics with...will run you about $2-3,000/laser. However, an important caveat is that one laser can stimulate only a ... on optogenetics we’ll delve into some of the important considerations when performing optogenetics behavioral...writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow...

...subcellular localization and composition of cellular complexes are important steps in understanding the ...Once you've created your fusion protein, it's important to validate its function relative to the untagged...experiments in a scientific and meaningful way it is important to choose the appropriate quantification methods...require some consideration of FP properties. It is important to look out for general problems associated with... for fluorescently tagged viral and bacterial components. For example, the Rainbow Vectors from the Mariette...

...Preparing for the first meeting There are many important pieces before that first meeting takes place to... to ensure success. One of the most important is letting people know it’s happening. Aim for 3-4 weeks...technical dive on single papers could be more important.  Any slides, presentations, recordings, and other...yourself in this situation, considering stopping (temporarily or permanently) the recurring meeting or perhaps...be well planned and carefully organized. Most importantly, they should be regularly assessed for continued...some of the information described above you feel empowered to start your own journal club! Additional resources...

... are more likely to consider cover letters an important part of the application. Even within an organization...core skill, considers cover letters to be very important. On the other hand, our app/dev team rarely, if...that organization or group. This is particularly important for labs and nonprofits, who tend to prioritize... research], and have excellent [list the most important skills for the position]. I am enthusiastic about...about [something that you love and is important for the job]. Notice that the intros above allow you ...that they considered cover letters to be more important for higher-level positions. Many did not require...

...antibiotic compounds. In such a case, the microbe itself would be unharmed by the compound it produces...this post will, indeed, focus on bacteria, it is important to note that the term ‘antibiotic’ can also be...treat infections, we are treating microbes with compounds similar to those they have been battling for centuries...animal farming is already being recognized as an important step, as you can’t walk into any supermarket now...hygiene standards. Handwashing is an essential component of preventing infection, as is cleaning and disinfecting...

...point is there are many ways to quantify the composition of a viral vector solution, and generally these... characteristics give us information on three important factors. What can AAV titers tell you? Physical...dosing purposes in preclinical studies, it is important to understand what these values mean and how they...low levels of background protein and chemical components, ddPCR may not be an improvement over qPCR (Taylor... lab. The absolute titer values may not be as important as the relative titer values. Finally, because...PMC2900506. Additional Resources on the Addgene Blog Important Considerations When Using AAVs Addgene's AAV Quality...

... of working in industry and how delegating can empower people Chapter 3: How Crystal advanced at Novartis...Chapter 4: Crystal's approach to management and the importance of realizing that different people have different...projects, and how she sets metrics for success. The importance of thinking about the big picture and not just... who are planning on working in industry. The importance of recognizing and leveraging your unique skills...skills and how they've helped her at Novartis. The importance of mentorship in developing career skills. Outro...

...optimized, it’s important to ascertain how long the rescue phenotype will last, and more importantly, if it does...genetic disease in a postnatal mouse model, an important proof of concept for future preclinical and clinical...desirable. Should stem cell editing occur, the CRISPR components would only need to be expressed for a short period...does not cause obvious germline modification, an important finding given the controversy associated with ...great step forward for CRISPR gene therapy, it’s important to realize that DMD is a simpler case than other...

...orchestrate the formation of high-functioning-but-temporary teams that have a specific purpose and finite ...year, or a few months, or even a few weeks. The important thing to remember is that every team should have...when I do come across one. Also, remember, one important feature that all projects have in common - and... What does a good team need? Besides that all-important sense of purpose, there are many more things necessary... function is certainly necessary, but equally important is making sure all team members understand what...their responsibilities are. That’s a subtle but important distinction. If I invite someone to be on a project...

...understand the specific components that make up a plasmid and why each is important. Our “Plasmids 101” series...molecular biology knowledge and techniques, and empower you with a firm understanding of the fundamentals...Drives transcription of the target gene. Vital component for expression vectors: determines which cell ...and selectively grown on antibiotic plates.  Importantly, because the bacteria from which plasmids are...

...because it will be difficult or (more likely) impossible to find; therefore all the time spent collecting...t guarantee a publication. There is an art to composing manuscripts and the ASM Scientific Writing and...writer, I was unsure if I should possibly take a composition class in the English Department. He suggested... coming to the writing workshop in D.C. had to compose and submit a manuscript that they were working .... Practice writing everyday Overall, the most important instruction the classes taught me was to write...

...from one man teams.  The composition of the management team is more important than the product itself ...CEO of miLab, a Boston-based startup focused on empowering early-stage scientists. You can think of miLab... that raising capital for such a fund is an impossibility at our early stage.  After each pitch we made...diverse educational backgrounds (an essential component to any successful startup management team). Sreyan...

...warfare [Vilensky & Redman, 2003]). Most importantly, these compounds cross cell membranes, thus enabling ...nonspecifically soak up your fluorophore package. One important concept in FRET experimental design is that the...donor first where labeling specificity is most important. Donor labeling and the need for specificity Donor...protein molecules contain the donor and, more importantly there is no fluorescence background resulting...contrast can be improved using disulfide-containing compounds such as British anti-Lewisite (which was originally...