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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 336 results for: nam

Showing: 41 - 60 of 336 results

  1. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...start by asking two questions: 1) Is the sample dynamic or static? 2) Is the sample thin (< 15 μm) or thick...day fluorescence microscopes (see Table 1). Thin dynamic samples Ex: monolayers of live cells with fluorescently...as a scientific CMOS camera. If plasma membrane dynamics are the main focus of the experiment, a Total ...in resolution, down to 10s of nanometers. Thick dynamic samples Ex: 3D cell cultures, small model embryos...

  2. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...control experiments are needed to establish the dynamic range of your assay. These experiments include ...values you can expect, thereby establishing the dynamic range of your FRET assay (Figure 2). These labeling...PMCID: PMC2735349. 8. Miyawaki, Atsushi, et al. "Dynamic and quantitative Ca2+ measurements using improved...Titov, and Jin Zhang. "Analyzing protein kinase dynamics in living cells with FRET reporters." Methods ...

  3. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...PX459) V2.0: What's not in the name? Not included in this plasmid's name are some additional features. ...CRISPR nuclease, SpCas9 from the Zhang Lab. It's name, though a little confusing to the uninitiated, contains...

  4. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...gRNA, facilitating the live study of chromatin dynamics at a particular locus. A limitation of this system...used to examine both inter- and intrachromosomal dynamics, and how they change in response to the cell cycle...the plasmids from this publication at Addgene. Dynamic imaging of genomic loci in living human cells by...

  5. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...backbone contains a kanamycin resistance cassette, you would plate the cells on kanamycin-containing agar..... Only cells carrying plasmids that contain a kanamycin resistance cassette will grow. However, often ...

  6. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...Cellosaurus is searchable by cell line name (recommended names and synonyms will all get you to the right...an 'h' or a 'b' written on the flask (and if the name was the correct one!)    Fig. 1: HeLa cells...

  8. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...this single mRNA; you get a view of translational dynamics at the single molecule level. The coding sequence...methods increase our understanding of translation dynamics, but they come with a few caveats. In both cases...this paper at Addgene. 3. Yan, Xiaowei, et al. “Dynamics of Translation of Single mRNA Molecules In Vivo...

  10. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, or Z. Some...user can search by sequence, category of enzyme, or name to discover all the information about that restriction...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...precise genome modification. Plasmid ID Plasmid Name Cas9 Variant Speacial Features 85169  pJL-SaBE3... the B. subtilis Single Gene Deletion Library—Kanamycin and the CRISPRi Essential gene Knockdown library...regions (excluding start and stop codons) with kanamycin cassettes and were carefully designed to allow... a Kruppel-like zinc finger protein (previously named ZBTB48) in regulating the upper-bound of telomere...

  12. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...provide timely feedback, leading to unproductive dynamics. I experienced this firsthand when it took me ...and online courses focused on leadership, team dynamics, and project management. Mentorship is another...or DISC assessments can help you navigate team dynamics effectively and foster a cohesive, productive ...

  13. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...official” name for the container to be used in cross-team communication A list of “AKA” names, or jargon...other relevant information Prescribing an official name to all of our plates, tubes, and racks meant we ...

  14. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...Fluorescent protein based FRET pairs with improved dynamic range for fluorescence lifetime measurements." ... monitor neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based Biosensors on Addgene's...FRET for understanding biomolecular structure and dynamics in live cells." Current opinion in structural ...

  15. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... with the ID of the component plasmid, component name, size, and any comments. You can use the ID to look...plasmids on the Addgene website. Step 4: Naming your assembled plasmid and generating the vector...step, Step 4, is to give the assembled plasmid a name and generate the vector file. Ta-da! Most (180/181...

  16. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...triple mutant, Y187F, R144I and T155Q, which they named QC2-6 FIQ or mStayGold (Ando et al., 2023). This...original (Ivorra-Molla et al., 2023). They also named their variant mStayGold. It is worth mentioning ... engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery...

  17. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...Neurons, for example, have unusually fast calcium dynamics, whereas non-neuronal systems tend to have slower... expression (CAG promoter). An improved version named CaMPARI2 is available for neuron-specific expression...calcium indicators to probe complex brain circuit dynamics in vivo. Neuroscience Research. 2021 Aug 1;169...

  18. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ... mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George G. Stokes) is the shift in wavelength...denoted by a “m” as the first letter in the protein name, e.g. mCherry). Oxygen: The maturation of the chromophore...study gene expression, nuclear localization, and dynamics” Curr. Opin. Cell. Biol. 23:310. ...

  19. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...luck, but I’m feeling optimistic for the next tournament). Minus a little rain towards the end, it was...completely new realm. It allows for the usual office dynamics to be mixed up a bit. Who know that the quiet ...also with having a good time. Host a board game tournament or beer hour (only if it’s cool with your legal...

  20. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...in technical support have? There are different names for technical support roles. Examples are Technical...corners in a company, less exposed to the fast dynamics and pressure which are often found in sales or...assurance, product management, marketing, and sales (to name a few). Depending on the particular company structure...
Showing: 41 - 60 of 336 results