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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 628 results for: rel

Showing: 41 - 60 of 628 results

  1. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ... protein fluorophores and quenchers are still relatively bulky. One of the advantages of oligonucleotides...hydroxybenzylidene imidazolinone (DFHBI) with relatively low fluorescence in vivo, the Jaffrey lab used...incredible versatility opens aptamers for use in many entirely new applications including aptamer based fluorescent...same effect can be achieved for a fluorophore that relies on dimerization for activation, only in this case...case ligand binding would bring the relevant ends together rather than separate them (Fig. 1b). One can...until they bind to the appropriate ligand, but relying on a lack of fluorescence for readout may render...

  2. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...physically separates molecules while maintaining their relative positions, allowing you to resolve individual ...accessible imaging equipment, ExM protocols utilize relatively inexpensive reagents and are readily adoptable...they want to understand the ultrastructural relationships that influence everything from cell division...optimization and careful execution, but they cannot be entirely eliminated. It is recommended that you image samples...

  3. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...construct [1,2], i.e. positioning of the FP sequence relative to cell compartment targeting sequences, which...to characterize FP-fusion proteins functionally relative to the untagged protein of interest. To avoid ...

  4. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...provided as an explanation for why they are paid relatively low wages (Stephan estimates that postdocs are...that PIs no longer save grant money by paying relatively low postdoc salaries as opposed to Staff Scientists...

  5. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ... model organism for scientists as they have a relatively short generation time for mammals - the time ...that are laid externally. Fruit flies are also relatively straightforward to manipulate genetically. Scientists...Yeast are also the first eukaryotic genome to be entirely sequenced and is very amenable to genetic manipulation... zebrafish reference genome sequence and its relationship to the human genome." Nature496.7446 (2013):...

  6. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...editing efficiency based on the location of the ‘A’ relative to the protospacer adjacent motif (PAM). ABE7.10...edit in a stretched window ranging from 1-17 bp relative to the PAM sequence (Zong et al., 2018) (see CBE...pathway which occurs in cells at low frequencies and relies on the efficient delivery of donor DNA to facilitate...CBE. Base editors for the plant genome These preliminary studies demonstrated successful base editing ...

  7. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...rat, is another popular model organism. A close relative to the mouse, both are great mammalian models ...that make them an ideal model organism; they are relatively simple to maintain and breed and they have large...BSL-1 status, E. coli can be used as a model to relate these processes in more pathogenic bacteria (Blount...regeneration.  In addition, axolotls have many age-related phenotypic changes that are similar to those observed...axolotl genome, revealing an abundance of genes related to limb regeneration (Vieira et al., 2019). To ... ability for scientists to study human disease-relevant genes in such a controlled environment displays...

  9. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...fleeting. “What’s been shown by others is that, in a relatively short amount of time, cancers become resistant...to activate each of these pathways. Rather, they relied on published studies in the literature to curate... library to include activators for more cancer-related pathways and more nodes within those pathways. ...

  10. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...both methods. These approaches have relatively low sensitivity, which can be problematic if you are trying...also influence antibody binding by altering the relative concentration of potential antibody binders and...recommendations for improving antibody use and reliability (Roncador, et al., 2015; Taussig, et al., 2018...your target then you would expect to see good correlation between the levels of target detected in both... targeting a different epitope, and look for correlation between the results. Using antibodies against...cytometry using both antibodies, as well as a third unrelated antibody as a negative control (A). The two antibodies...you very similar results, while the third looks entirely different (B), giving you confidence that your...

  11. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ... controls how many false positives are allowed relative to true positives. Both metrics can be used to...and plot your data in ascending numerical value relative to quantiles derived from a theoretical distribution.... Below are several core factors of statistics relevant for many downstream statistical tests.   Independent...significance exists somewhere amongst multiple groups is rarely enough information. A post hoc test, done after...yourself from family-wise error and just perform the relevant comparisons using Dunnett’s test, a test that ...

  12. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...post-translational modifications, quantify protein levels relative to standards, detect the cellular location of ...thickness of the band provides information about the relative abundance of the protein in the sample. More protein...endogenously expressed protein at physiologically relevant levels. Some vendors will also test their antibody...known to express the protein at physiologically relevant levels (not overexpression systems) while negative...molecular biology protocols Browse other articles related to antibodies Resources on Addgene.org Find written...

  13. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...you can use alumni databases, friends, and even relatives to network your way to a hiring manager. Learn...Expanding your network of relationships early and often is the most effective tactic a scientist in training...result of existing professional (and personal) relationships. However, most job seekers will and should apply...after the academic bench. Utilizing existing relationships as part of the job application process can dramatically...

  15. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...helped bring it to fruition. The concept, though relatively simple, changed the course of scientific research...

  16. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...mesothelioma that affects the lining of the lungs is relatively well known, there are a variety of lesser known...heart health. These are amazing advances that will surely save thousands if not millions of lives, but many...therapy for peritoneal mesothelioma and thereby relieve suffering from this little-known disease. You can...

  17. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...50–90% of average on-target DNA-editing levels relative to BE4max.  Read the paper in Nature Biotechnology...

  19. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...sequence. In contrast, Dre recombinase, the close relative of Cre, displayed an excision efficiency of only...mammalian genomes: genome-wide distribution and relevance for the efficiency of BAC/PAC recombineering techniques...
Showing: 41 - 60 of 628 results