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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 262 results for: rigi

Showing: 41 - 60 of 262 results

  1. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ... really need to use FACS (like they did in the original publication). I thought I could see GFP expression...against GFP expression, but I didn’t. 2. In the original publication they see GFP expression in about 20%... cells at an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use multiple gRNAs to...

  2. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ... started engineering a CBE to be worse at its original job. They removed the uracil glycosylase inhibitor...could explain why the edited U base opposite the original G is not primarily converted back to a complementary... to improve chromatin accessibility One of the original labs that pioneered these editors (calling them...

  3. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ... This blog was originally published on BitesizeBio here. As part of my job ensuring plasmid quality at... Acknowledgements: Thank you BitesizeBio for originally publishing this and allowing us to share it with...

  5. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...the process a bit easier and more efficient. Our original policy was straightforward, but cumbersome: depositors...champagne or cupcakes, or maybe the lab member originally responsible for the deposit had already moved...

  6. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...known to be genetically manipulated, its anatomical origin, if cell line has ever been the target of an ‘-...about. If you have cell lines without a certified origin (you know, that split you got from the graduate...

  7. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...The resulting chimeric antibody would have the original variable regions from the parental rat antibody...antibody retains the binding specificity to the original target and can be used with secondary antibodies...

  8. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ... THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...prevent the invading genome from “recovering” its original sequence. If you’re looking to disrupt gene function...

  9. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ... antibiotic resistance markers and replication origins, but there is extensive heterogeneity in the feature.... In an effort to be rigorous, we dug into the original literature, some of it decades old, to provide...

  10. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...these materials may not be available from their original sources. Figure 1: Biological repositories...availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley Perlman...

  12. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...continue to produce the specific antibody that the original B cell produced. Because of this, all of the antibodies...reproducible experimental results. When you are originally selecting a hybridoma, you can choose one that...

  13. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ... lab, the vectors must contain a yeast-specific origin of replication (ORI) and a means of selection in...ORI and antibiotic selection markers.  The yeast origin of replication (Please note: This first section...

  14. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...to distinguish between RNA of cellular vs. viral origin and respond appropriately. When these sensing systems...10.1101/gr.237362.118 Chen, G. Y., Hur, S. Cellular origins of dsRNA, their recognition and consequences. Nat...

  15. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...overhangs. Entry DNA overhangs may be present in the original plasmid (Option 1) or added using PCR-based amplification...containing the Type IIS enzyme and ligase. Although the original destination vector + insert may spontaneously ...

  16. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...number of important plasmid elements – promoters, origins of replication, protein tags, and antibiotic resistance...use in genome manipulation. Cre recombinase, originally named because it “causes recombination” (although...

  17. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ... Editing, and that meant rereading many of the original papers reporting various prime editing tools. ...your curiosity, we encourage you to check out the original papers for more details. You can find many software...

  18. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...about some other common plasmid features, such as origins of replication and promoters, check out our other...exciting molecular technologies!  This post was originally written by Jessica Welch in January 2017 and ...

  19. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...post. From Mice to Men When this article was originally written, the main focus was on editing animal... and targeted therapy possible. This post was originally written in 2014 by Kendall Morgan and updated...

  20. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...immune system of bacteria. These Acr proteins were originally found in type I CRISPR-Cas systems.   Four years...
Showing: 41 - 60 of 262 results