Skip to main content
Addgene
Showing: 41 - 60 of 75 results

  1. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...cotransfecting them with a plasmid expressing PiggyBac transposase. In rtTA-cell lines, it is better to first engineer...the mARG cassettes 2:1 (A:N-V) with PiggyBac transposase and outgrow the cells for a several generations...

  2. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...new optogenetic tool for localized microtubule disassembly, Opto-katanin (Meiring et al., 2022), that overcomes... optogenetic tool for localized, microtubule disassembly. Current Biology, 32(21), 4660-4674.e6. https...

  3. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    .... Type I systems are also employed as CRISPR transposases, by leaving out Cas3. Type I is further subdivided...paradoxically called V-K, is a CRISPR-associated transposase (CAST) that can insert large fragments of DNA...

  4. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...in the experimental samples. Get more details on ELISAs in our article Antibodies 101: ELISA (Enzyme-linked...in a series of samples, such as Western blots or ELISAs, or for other experiments to study protein-protein...

  5. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...shows how antibodies are used to study proteins via ELISAs, western blots, immunoprecipitation, immunohistochemistry...

  6. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3 system. We also describe...

  9. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan @AnoeskaMoosdijk @ShebaAJ pic.twitter.com/X6QYXteRLT...

  10. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C but preferentially...

  12. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...don’t generally affect antibody applications like ELISAs or Western blots, they are key in helping antibodies...

  16. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western blotting.  The five isotypes of antibodies...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...
Showing: 41 - 60 of 75 results