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Showing: 41 - 60 of 133 results

  1. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...demonstrated that it can also detect expression of toxin mRNA from Clostridium difficile. As mRNA expression...methods, the ability to detect mRNA transcripts of toxins and other transcripts of interest is a benefit ...

  3. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...advancement over previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources... to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing...

  4. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less toxic to mammalian...

  5. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...cells remain difficult to genetically edit due to toxicity from electroporation or poor cellular entry with...frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as...

  6. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...extremely useful if a desired protein product is toxic to cells at high concentrations. Despite their advantages...lower expression may be required if your protein is toxic to E. coli. Of all the vectors, pET, under the control...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...increased particle stability; however, the protein is cytotoxic precluding the long-term expression of lentiviral...vectors in producer cell lines. Identifying less toxic envelope proteins or envelope proteins that target...

  8. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...correct? Is the purified plasmid DNA viable? Or cytotoxic? How and where did things go wrong? The use of...reagents or the transfection process itself has any cytotoxic effects on the target cells. Another type of transfection...

  9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...produce viral proteins and die, either through cytotoxicity or the immune response. The second strategy ...expression. In cell lines that can produce HIV-1 toxic proteins, CRISPR/Cas9 SAM caused apoptosis, indicating...

  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...problematic because excitation light can cause phototoxicity, autofluorescence, and perturb biological phenomena...the case of exciting cyan-excitable donors, phototoxicity. Another challenge to using FRET sensors comes...

  11. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ... overexpression of your gene and could lead to toxicity or trouble interpreting your results, while a ...low expression.  Finally, the risks of potential toxicity and/or off-target effects (e.g. recombinase-dependent...

  13. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...high 3D spatial resolution while also avoiding the toxic effects of high light doses to the cells. Thin static... its fast temporal resolution and decreased phototoxicity. Lightsheet designs that allow for multi-view...

  14. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...penetrate 5 mm past the surface of skin and is less phototoxic. Using the FAST system, Ye’s lab could edit a...editing, reduce off-target activity, and prevent cytotoxic effects. For a more detailed review, check out...

  15. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...  acrIIA3   Listeria monocytogenes  pCSW65 N/A (Toxic)   None N/A   acrIIA4 Listeria monocytogenes  ...  acrIIA3 Streptococcus pyogenes  pCSW24  N/A (Toxic) pJH375  No    ...

  16. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ...to share their new CRISPR tools, Cas7-11, a low-toxicity RNA editing tool, and PASTE, a drag-and-drop CRISPR...

  20. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...phage host factors, and identify sequence-specific toxicity of Cas9. Read the paper in Nature Communications...
Showing: 41 - 60 of 133 results