We narrowed to 139 results for: tox

...using this plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses...lentiviruses challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast...

... Background Lower Higher Photobleaching/phototoxicity Not susceptible Susceptible Subcellular imaging...live-cell imaging by avoiding autofluorescence and phototoxicity and even allowing multiple proteins to be visualized...

...demonstrated that it can also detect expression of toxin mRNA from Clostridium difficile. As mRNA expression...methods, the ability to detect mRNA transcripts of toxins and other transcripts of interest is a benefit ...

... cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less toxic to mammalian...

...advancement over previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources... to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing...

...spatial precision of gene expression and reduces toxicity as compared to previous Cre/lox or drug-induced...inactivating Cre. This is critical for reducing Cre toxicity in long-term experiments.  Figure 4: The...

...cells remain difficult to genetically edit due to toxicity from electroporation or poor cellular entry with...frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as...

...extremely useful if a desired protein product is toxic to cells at high concentrations. Despite their advantages...lower expression may be required if your protein is toxic to E. coli. Of all the vectors, pET, under the control...

...increased particle stability; however, the protein is cytotoxic precluding the long-term expression of lentiviral...vectors in producer cell lines. Identifying less toxic envelope proteins or envelope proteins that target...

...correct? Is the purified plasmid DNA viable? Or cytotoxic? How and where did things go wrong? The use of...reagents or the transfection process itself has any cytotoxic effects on the target cells. Another type of transfection...

...produce viral proteins and die, either through cytotoxicity or the immune response. The second strategy ...expression. In cell lines that can produce HIV-1 toxic proteins, CRISPR/Cas9 SAM caused apoptosis, indicating...

... overexpression of your gene and could lead to toxicity or trouble interpreting your results, while a ...low expression.  Finally, the risks of potential toxicity and/or off-target effects (e.g. recombinase-dependent...

...generating reactive oxygen species that can lead to phototoxicity, perturb biological processes, and compromise...Reynaud, E. G., & Shroff, H. (2017). Assessing phototoxicity in live fluorescence imaging. Nature Methods...

...problematic because excitation light can cause phototoxicity, autofluorescence, and perturb biological phenomena...the case of exciting cyan-excitable donors, phototoxicity. Another challenge to using FRET sensors comes...

...high 3D spatial resolution while also avoiding the toxic effects of high light doses to the cells. Thin static... its fast temporal resolution and decreased phototoxicity. Lightsheet designs that allow for multi-view...

... the brains of mice chronically infected with Toxoplasma gondii." The Journal of Immunology 182.10 (2009...cells and dendritic cells during infection with Toxoplasma gondii." PLoS Pathog 5.7 (2009): e1000505. PubMed...

...penetrate 5 mm past the surface of skin and is less phototoxic. Using the FAST system, Ye’s lab could edit a...editing, reduce off-target activity, and prevent cytotoxic effects. For a more detailed review, check out...

...  acrIIA3   Listeria monocytogenes  pCSW65 N/A (Toxic)   None N/A   acrIIA4 Listeria monocytogenes  ...  acrIIA3 Streptococcus pyogenes  pCSW24  N/A (Toxic) pJH375  No    ...

...to share their new CRISPR tools, Cas7-11, a low-toxicity RNA editing tool, and PASTE, a drag-and-drop CRISPR...

...antibodies, for example through the use of less toxic adjuvants; 2) generating complex antibody chips ...