We narrowed to 106 results for: transfection

... your packaging cell line, prepped your DNA, transfected and harvested your lentivirus. Now it’s time ...the correct transfer plasmid was used in the transfection; if preparing several different lentiviruses...

...ratio of acceptor to donor fusions varies with transfection efficiency and any unpaired fluorescent proteins...measure (overexpressing a gene or protein after transfection). Future of FRET The first genetically encoded...

...BiFC constructs are expressed transiently by transfection or stably impacts the success of BiFC experiments...dose-dependent manner. This is straightforward in easy-to-transfect cell lines where plasmid dosage can be tightly...

...in mammalian cells is relatively quick. After transfection, HEK-293T cells start producing virus within...systems, it is standard to do only one passage (transfection). This differs from typical insect cell preparations...

...be produced through a single or dual-plasmid transfection, depending on whether the heavy and light chain...they’ve been separated onto two. You’ll first transfect your cells with your plasmid(s), and then wait...

...the University of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a ...primers targeting the transfer plasmid used in the transfection. PCR products are analyzed by gel electrophoresis...

...packaging and host genome integration. After co-transfection of these plasmids, 293T cells released transgene-containing...work with primary cells or other difficult-to-transfect cells. Lentiviral barcoding libraries have been...

...identical targeting vectors delivered through transfection of naked DNA. Other investigators who had been...then packaged into AAV virions via transient transfection of 293T cells with helpers, and used to infect...

...generating hiPSCs for clinical purposes: mRNA transfection- this allows transient expression of the reprogramming...express reprogramming factors To date, the mRNA transfection method has not been used successfully to derive...

...site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA sequences...efficacy of your gRNAs is then a simple matter of transfecting HEK293T cells with your modified pCAG-EGXXFP...

...the passage number of a culture the lower the transfection and transduction efficiencies. Cultures should... while others opt for a single harvest after transfecting with the transfer and packaging plasmids. A ...

...as effective NIR fluorescence in transiently transfected live HeLa cells with no supply of exogenous BV...and after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0...

...delivered as any type of small RNA and can occur via transfection or viral delivery of a plasmid. RNAi is an umbrella...mature siRNA. siRNAs are delivered via transient transfections and are handy for quick in vitro experiments...

...Transformation, Transduction, Bacterial Conjugation, and Transfection by Alyssa Cecchetelli The introduction of nucleic...

...target cells that have previously been stably transfected for Cas9 expression. The Yusa lab has used their...

...multimers may exhibit reduced transformation and transfection efficiency compared to the monomeric version... Hartl D, Rudolph C. Transgene expression of transfected supercoiled plasmid DNA concatemers in mammalian...

...transducers. To use the TRUPATH system, cells are transfected with plasmids expressing the GPCR of interest...

...Harrington et al tested GeoCas9’s editing skills by transfecting HEK293 cells with GeoCas9 or SpCas9 RNP’s and...

...in the cell. gDNA guides will also need to be transfected into the cells of interest. In contrast, RNA ...

...and enrichment use of XMAS-TREE. a) Cells are transfected with three different vectors and flow cytometry...