We narrowed to 75 results for: ubc

1. Fluorescent Proteins 101: Luciferases

   Type

   Blog Post

   Published

   July 22, 2025, 1:15 p.m.

   .../phototoxicity Not susceptible Susceptible Subcellular imaging Improving Well-established High-throughput...

2. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   Published

   June 1, 2017, 2:30 p.m.

   ...Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire Fluorescent Protein...

3. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   Published

   May 25, 2017, 2:30 p.m.

   ...phototoxicity, they successfully imaged multiple subcellular compartments over several minutes using Nano-...

4. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   Published

   Dec. 12, 2019, 2:22 p.m.

   ...educational resources, and depositors — each with subcategories such as plasmids and viral preps for catalog...

5. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

   Type

   Blog Post

   Published

   Dec. 6, 2018, 1:34 p.m.

   ...to studying membranes, organelles, and other subcellular compartments is via proteomics--the study of ...

6. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...the same cell, but not necessarily at the same subcellular compartment as the opsin or chemogenetic receptor...

7. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   Published

   June 15, 2017, 2:30 p.m.

   ...Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire Fluorescent Protein...

8. Choosing the B(right)est Fluorescent Protein: Photostability

   Type

   Blog Post

   Published

   June 8, 2017, 2:30 p.m.

   ...Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire Fluorescent Protein...

9. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   Published

   March 19, 2019, 1:31 p.m.

   ...photo-reversible protein-protein interactions can control subcellular localization processes in vivo. They deposited...

10. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...recombinase. Addgene has several great options: pLV hUbC-dCas9-T2A-GFP for removable dCas9 expression and...

11. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    Published

    May 4, 2021, 1:15 p.m.

    ...Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

12. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ...distribution of clusters without accumulation in subcellular compartments such as nuclear speckles. Furthermore...

13. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    Published

    March 15, 2016, 2:30 p.m.

    ...sorting based on expression of a surface marker, subcloning, expansion, and confirmation. This procedure ...

14. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ... where specific tissues, cell types, or even subcellular regions of a neuron need to be targeted. Targeting...

15. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ...-wrmScarlet labeling proteins with different subcellular locations. Image from Goudeau et al., 2020. ...

16. Viral Vectors 101: The Retroviral Lifecycle

    Type

    Blog Post

    Published

    July 27, 2023, 1:15 p.m.

    ...been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (like ...

17. Negative Can Be Positive: Open AAV Data with Addgene

    Type

    Blog Post

    Published

    Sept. 6, 2022, 1:15 p.m.

    ...and Share AAV Data with Addgene's New AAV Data HubCelebrate Open Data Day with Addgene’s AAV Data Hub!Data...

18. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    Published

    April 7, 2016, 2:30 p.m.

    ...transductions. Cell lines should be routinely subcultured at densities that are low enough to prevent confluency...

19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ... in vitro without antibiotic selection. In a subcutaneous cancer model, the researchers showed that after...

20. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    Published

    Aug. 14, 2018, 12:42 p.m.

    ...structures GFP is limited in its ability to label subcellular structures in the CNS. GFP and other FPs have...