Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 260 results for: uros

Showing: 41 - 60 of 260 results

  1. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... stem cell-derived neurons expressing ASAP5-Kv. C) Cultured rat hippocampal neuron expressing pan-membrane...synaptic events. Neuron, 112(22), 3680–3696.e8. doi: https://doi.org/10.1016/j.neuron.2024.08.019.  ...indicator ONE-GO biosensors kit Antibodies for neurodegeneration research   Optimized prime editing in dicots... gene to confer resistance to the herbicide chlorsulfuron (Homo line 2) or wild type (WT), two weeks after....01.028. New recombinant antibodies for neurodegeneration research   By Ashley Waldron We recently added... antibodies against important targets for neurodegeneration research: Anti-MAPT [AT8] and Anti-APP [6E10...in tissues from patients who had confirmed neurodegenerative diseases (Alzheimer’s disease or Hoarding ...

  2. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...improve the study of specific neuronal cell types involved in neurodegenerative diseases, the Cole Lab selected...linked to disease, including motor neurons (-3mnx1/Hb9), pan neuronal (elavl3/HuC), microglial/macrophage...specific neuronal subtypes was not available until now. In order to target specific neuronal subtypes ...infecting GABA-ergic interneurons here. Dimidschstein J, et al. Nature Neuroscience. 2016. PubMed PMID:...generate zebrafish transgenic lines to study neurobiology and neuorodgenerative diseases. The Tol2 Gateway-Compatible...), and astrocytic (gfap).This toolbox adds new neuronal tools to the expanding gateway compatible vectors... PMID: 25772473 Targeting and manipulating interneurons using a viral approach Genetically modified...

  3. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...great in a GABA neuron, for instance, may not transduce the adjacent norepinephrine neuron at all. There... for Defense Behaviors. Neuron, 93(1), 33–47. https://doi.org/10.1016/j.neuron.2016.11.045 More resources...expression can still occur (Fig. 3) - for example, some neurons can transiently express TH - so you’ll need to ...expression in both oligodendrocytes (Olig2) and neurons (NeuN). Image from Griffin et al., 2019, Figure...central nervous system. Frontiers in Molecular Neuroscience, 7. https://doi.org/10.3389/fnmol.2014.00076...cFork anterograde tracing system. Experimental Neurobiology, 29(3), 219–229. https://doi.org/10.5607/en20006...

  4. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...named NeuroStack was used (Orguc et al., 2020). Using a programmable microcontroller, NeuroStack coordinates...and stimulate or manipulate responses through neurological means like electric stimulation, drug administration...optogenetic tools, calcium sensors, and other common neuroscience tools into the brains of model organisms, including...tissue. They performed optical stimulation using NeuroStack and the fibers as previously described, then ...trended with expression of the opsin in the target neurons, validating the functionality of these inputs and...Success! They also observed reliable optically evoked neuron activity for at least several months after implantation...enteroendocrine cells within the intestines known as neuropods. They observed increased vagus nerve firing in...

  5. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...biologists, while neuroscientists would like to monitor calcium specifically in their favorite neurons. This specificity... V., Fluorescent Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers... or animal system. This ‘thing’ could be a neurotransmitter, such as norepinephrine, or an electrolyte...contraction, and calcium channels are activated for neuronal action potentials; it’s an ion of interest for...—keep reading to find out how!   Neurotransmitters Many canonical fluorescent as well as FRET-based...been engineered to detect a wide array of neurotransmitters ranging from dopamine to glutamate, all the... Frontiers in Cellular Neuroscience. 2019 Oct Vol. 13; 1662-5102. DOI: 10.3389/fncel.2019.00474 Resources...

  6. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...oligodendrocytes, microglia, astrocytes, neurons, and inhibitory neurons. Your choice of AAV will depend upon... as a new tool for neuroanatomical tracing (11). With the ability to produce neuron specific transfection...contributed by guest blogger Katrina Armstrong, a Neurophysiology Msc Student at the University of Manitoba. ... These AAVs may move toward the cell bodies of neurons (retrograde transport) or towards the synapse (...Cre-lox, this new method could allow for labeling of neurons showing a very specific phenotype. Previous neural... Katrina Armstrong. Katrina Armstrong is a Neurophysiology Msc Student at the University of Manitoba. ... Floris G., et al. "A fourth generation of neuroanatomical tracing techniques: exploiting the offspring...

  7. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...cell types (astrocytes, microglia, neurons, etc.) and even neuronal phenotype in a mixed population (Burger...Many neuroscience experiments that require gene expression in a specific cell type rely on transgenic...tyrosine hydroxylase (TH) promoter for catecholamine neurons or glial fibrillary acidic protein (GFAP) for astrocytes...vectors for off-target expression.  Some classes of neurons transiently express TH during development or have...there is promoter activity. Additionally, some neurons express low levels of GFAP, which can lead to off-target...off-target expression of the packaged transgene in neurons and glia.   Check the recombination rate The first...on Helper Virus Concentrations. Front Synaptic Neurosci 12. https://doi.org/10.3389/fnsyn.2020.00006 O...

  8. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...coffee does it take to fuel more than 30,000 neuroscientists over a 5 day conference? I feel like here at... at the annual Society for Neuroscience meeting in Washington, D.C., we must be making a dent in the global...conference, I have been impressed by how the neuroscience community pulls off such an inspiring, thought-provoking...made in reprogramming astrocytes to functional neurons in vivo. And I’ve seen evidence of the infiltration...infiltration of CRISPR-Cas tools into neuroscience experiments. Speaking of CRISPRs, we were excited to have... the importance of scientific sharing to the neuroscience community. The scientists we've spoken with ... for a free plasmid! Also check out the PLOS Neuroscience Community blog to read reports from bloggers...

  9. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ... directed by a gRNA. 2A-Puro In this plasmid, SpCas9 is fused to the puromycin resistance gene through...pSpCas9(BB)-2A-Puro (PX459) V2.0 from it's predecessor, which had a point mutation in the puromycin resistance...most request plasmid in 2016 was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last year, the top...through a 2A peptide. The puromycin resistance gene enables selection of cells with the plasmid post transfection...causes the ribosome to translate Cas9-2A and the Puromycin resistance gene as separate peptides. PX459 This...mutation has been corrected in V2.0. pSpCas9(BB)-2A-Puro (PX459) V2.0: What's not in the name? Not included...

  10. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...., et al. (2023) Neuron, 111(10), 1547–1563.e9. doi: https://doi.org/10.1016/j.neuron.2023.03.009. PMID...microglia The REVeRT Dual AAV Vector System Neurodegeneration Collection updates   Multiplexed perturbation... vectors for CROPseq-multi using selection by Puromycin, Zeocin®, or nuclear expression of BFP2.   ...fidelity during action potentials of fast-spiking neurons. The same A-to-D mutation in the related voltage...vectors and are suitable for extended imaging of neurons in vivo. Because they are negative-going sensors... the vector to silence transgene expression in neurons and other non-microglia cells.      Figure...42386-0. PMID: 37852949. New and notable Neurodegeneration Collection updates Last but not least, check...

  11. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...continues to supply neuroscience with ever more efficient tools for light mediated neuronal control and we ...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies on...photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids from...calcium dynamics that govern such activities as neurotransmitter release is vital in studying neural networks... on accurate monitoring of neurotransmitters and we are excited to offer AAV for the newest GRAB sensors...tool from Loren Looger’s lab at Janelia for neuroscientists to measure glutamate levels. iGluSnFR.A184S...

  12. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    Published
    May 6, 2014, 1:07 p.m.
    ...Oliver Griesbeck of the Max Planck Institute for Neurobiology has been working on genetically encoded indicators...monitoring the activity and biochemistry of living neurons in an effort to understand the connection between...considers “one of the greatest challenges of neuroscience.”  Hot plasmids: Twitch sensors Griesbeck’s...available beam splitters. In addition to imaging neurons, Griesbeck adds that this color change has a particular...refined by a secondary screen in rat hippocampal neuron cultures. The optimized Twitch sensors allowed ...researchers to see tonic action potential firing in neurons and high resolution functional tracking of T lymphocytes...them a versatile tool for application in both neuroscience and immunology. Don’t be shy: Ratiometric imaging...

  13. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...be used for sparse labeling of neurons for tracing experiments.  Neuronal tracing experiments: While synthetic...broad number of cell types, such as inhibitory neurons, rods, cones, or retinal ganglion. Rather than ...that produce sparse labeling would be useful for neuronal tracing experiments where lower levels of labeling...labeling makes it easier to trace a neuron’s projections. Generating animal models: Transgenic animals allow...for retinal gene therapy Find more about precise neuronal targeting using AAV technologies Browse all of... H.P., Krol, J., & Roska, B. (2019). Targeting neuronal and glial cell types with synthetic promoter AAVs... mice, non-human primates and humans. Nature Neuroscience, 22, 1345-1356. https://doi.org/10.1038/s41593...

  14. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...pushed the boundaries in neuroscience by granting targeted control over neuronal activity. While they serve...scale of milliseconds, and can precisely link a neuron's activation to behaviors. Scientists continue to... mutated to have faster off kinetics and allow neurons to repolarize faster.  Chemogenetics does not offer...Administering the drug once can result in several hours of neuronal excitation or inhibition. This sustained activation...tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches excel at discrete cell ...precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad control...

  15. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ... reagent resource for the neuroscience community called NABOR (Neuroscience AntiBody Open Resource). We...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative, will allow Addgene to...thousands of high-quality antibodies/nanobodies for neuroscience research and is an expert at validating these...While the focus of the NABOR platform will be neuroscience reagents and data, the platform can be expanded...are among the most commonly used reagents in neuroscience research today, but there is a critical unmet... to create recombinant antibodies for use in neuroscience research. More broadly, advances in gene synthesis...

  16. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...Access to Projection Neurons. Neuron, 92(2), 372–382. https://doi.org/10.1016/j.neuron.2016.09.021 Walters..., 2023). It may be a good choice for targeting neurons, with high transduction frequencies in the central... is derived from AAV2 and engineered to infect neurons and undergo retrograde transport, traveling up ...to target the central nervous system, including neurons, AAV5 is generally the best choice for targeting... year (Dhungel et al., 2025)! It can transduce neurons for retrograde labeling, as well as astrocytes ...enables efficient retrograde targeting of projection neurons and enhances astrocyte-directed transduction. Nature...

  17. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...change rapidly during neuronal activity, scientists can use GECIs to watch neurons fire under the microscope...indicators (GECIs) have become a vital tool in neuroscience research. These fluorescent proteins light up...challenge: Expanding the fluorescent toolbox Modern neuroscience experiments often rely on multiplexing, where...responses to increasing numbers of evoked APs in neurons expressing OCaMP or jRGECO1a. Reproduced from Aggarwal...including detailed characterization as protein or in neuronal cultures, zebrafish, and mouse brain, check out...Blog Viral Vectors 101: Calcium Sensors New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor A Practical...

  18. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    Published
    May 11, 2021, 1:15 p.m.
    ...are powerful tools to modulate the activity of neurons and other brain cells. Since the opsins or chemogenetic... we used AAV viral vectors to express hM4Di in neurons of rhesus monkeys or mice (Galvan et al., 2019)... electron microscope showed us that, in monkey neurons, the large majority of the gold particles were ...found a larger proportion on the membrane in mice neurons, most of them were still cytoplasmic. Use of ‘small...hM4Di‐mCherry at the plasma membrane in monkey neurons, we next hypothesized that a different tag fused...instance, when the fluorescence expressed in the neuropil conceals identification of opsin-expressing cells...Ultrastructural localization of DREADD s in monkeys. Eur J Neurosci 50:2801–2813 . https://doi.org/10.1111/ejn.14429...

  19. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...presynaptic region of one neuron and postsynaptic region of a different neuron, come into proximity in ...Druckmann et al., Neuron. 2014. Feb 5;81(3):629-40.  Next-Gen Brainbow toolkit for neuronal imaging Joshua...perform independent two-color optical excitation of neurons. The paper describes five previously unknown ...allowing labs to precisely and non-invasively control neuronal signaling. For more information on DREADDs, ...Addgene's Genome Engineering Guide. Fire up those neurons: mGRASP A Nature 2012 article by Jinny Kim and... use of another set of mGRASP plasmids in their Neuron 2014 paper. Kim et al., Nat Methods. 2011. Dec...high-resolution fluorescent imaging of individual neurons. The technology generates a unique spectral identity...

  20. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...norepinephrine (NE) expressing neurons and GFP (green) to label neurons transduced by each serotype. ...challenge to scientists. What works well in one cell or neuron type may not work well for a neighboring cell type...it to publish!  Working with AAV vectors in neuroscience Let’s consider my first challenge when I started...express ChR2(H134R) in A2 norepinephrine expressing neurons. We tested four serotypes containing an expression...84%) were both excellent at infecting our target neurons (Figure 1). However, this one set of experiments...in Deep Brain Stimulation. Frontiers in Human Neuroscience, 14(December), 1–12. https://doi.org/10.3389...
Showing: 41 - 60 of 260 results