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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 836 results for: Eras

Showing: 581 - 600 of 836 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...enter cultured cells and activate expression of a luciferase reporter. CPP was not required for protein entry...cells! After verifying the specificity of their luciferase assay, they concluded that components in the ...lethality and were sorted into single wells to generate clonal populations. After iTOP treatment, both...

  2. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...However, careers outside of the lab highlight “transferable” skills such as leadership, management, and ...impact your work? What design variables need consideration? Enrich your knowledge and skills outside of...tools such as the Individual Development Plan to generate a list of potential professions Google potential...

  4. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...-rings are, in fact, permeable to CO2 at low temperatures (-80°C) and once in the tube, the CO2 can alter... storage, we then thawed the samples at room temperature and used the color of the liquid solution and...not all O-rings) were permeable to CO2 at low temperatures. We tested two different types of tubes: the...

  5. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...landmark Cell paper showing that it was possible to generate induced pluripotent stem cells (iPSCs) capable...has spent years studying how that one protein interacts with that one promoter can tell you, it can be...clinical trial patients with advanced macular degeneration; Laurenz Studer discussed his work with Parkinson's...

  6. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ... with a simple question: how do Cas9 and sgRNA interact with and dissociate from a DNA target? The surprising...et al., 2016. We next wondered if we could leverage this model (built from in vitro studies) to boost.... Figure 2: Single-stranded donor DNA interaction with the Cas9-DNA duplex. Image from Richardson...

  7. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...minor variation in experimental conditions will derail a research program (think a subtle difference in...to help your fellow researchers advance science overall. Communication is the issue Much of the problem...President. Her current responsibilities encompass overall leadership of the Cell Press editorial department...

  8. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ..., why it affects our emotions, and even what therapeutic properties it has. As with other works of Dr....of characters from five different families over several millennia as their paths cross with each other ...Nicholson Baker’s The Anthologist. The book ended up literally changing my life. Though I have an undergraduate...

  9. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...reading, you can calculate the DNA concentration. Generally, A260 of 1.0 is equivalent to 50 ug/ml pure dsDNA...samples and are often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, .... This method is often used preceding next-generation sequencing or microarray studies and is not used...

  10. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... need a second GFP antibody?  Similarities:  Generated against full length GFP and converted to recombinant... also withstands denaturing conditions, high temperatures, and other chaotropic conditions better than...fluorescence in high molarity GdnHCl, at high temperatures, and in the presence of oxidizing agents. Adapted...

  11. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...in a cell or animal model. AAV plasmids contain several important elements, but among the most important...occurring.  How do I validate my ITR sequences?  Next-generation sequencing and Sanger sequencing might fail to...vector stability and sequencing results. Next-generation sequencing is also helpful in quantifying ITR...

  12. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...out of these interactions, ask yourself how every piece of feedback you receive generalizes.  If someone...skillset rather than a permanent fix. Treat every interaction in your work as an opportunity to practice communicating...

  13. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ... how stretches of DNA on different chromosomes interact with each other. Chromatin regulation is complex...in the right direction! CAPTURE-ing Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation... An Improved Method for Studying Protein-DNA Interactions CRISPRainbow and Genome Visualization CRISPR...

  14. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...and UAS plasmids at Addgene Choosing a vector to generate a transgenic fly There are a few important points... when choosing the vector you’d like to use to generate your new fly line: 1. Where would you like the...  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut. Cell...

  15. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... the “Analyze Sequence” button to pull up an interactive plasmid map powered by SnapGene and gain access...the static maps discussed above, but also have interactive functionalities. You can now hover over any feature...maps, the linear sequences hosted here are now interactive and have similar hover over features. As with...

  16. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...protein is based on the properties found in the literature or tabulated data that summarizes properties ...). However, the theoretical brightness ignores several important experimental conditions that are related...detector sensitivity) and the biological system (temperature, prokaryote versus eukaryote, background fluorescence...

  17. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...Transfection is the process by which foreign DNA is deliberately introduced into a eukaryotic cell through non-viral...diethylaminoehtyl (DEAE)-dextra neutralize or even impart an overall positive charge on DNA molecules so that it can.... "Chitin induces natural competence in Vibrio cholerae." Science 310.5755 (2005): 1824-1827. PubMed PMID...

  18. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...talk at the Cell Symposia: Gene- and Cell-based Therapies: CRISPR, Stem Cells, and Beyond meeting earlier...III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed until the base editor...to an uninfected culture which results in less overall dilution and less stringent phage selection. ...

  19. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ... the trace; asterisk indicates a single AP. B) Averaged fluorescence responses to increasing numbers of...International license. Using OCaMP in your lab Several OCaMP constructs are now available through Addgene.../article/28248224/","type":"EXTERNAL"},"user_generated_content":false}{% endraw %}{% end_module_attribute...

  20. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...closely associated and function as one unit: Gβγ. In general, GPCRs show a propensity to associate with certain...receptor, which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed ...Chemogenetics uses genetically engineered receptors to interact with small molecules to elicit a response in a...
Showing: 581 - 600 of 836 results