We narrowed to 610 results for: REV
-
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies.” Chem Biol...
-
Using Video to Share Your Science: We Share Science
TypeBlog PostPublishedNov. 3, 2016, 2:30 p.m....journal article, find blogs about the study, or even review Facebook and Twitter comments. On We Share science... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m.... Labor to update the FLSA, which had not seen revisions since 2004. In July 2015, the Department of Labor... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog PostPublishedOct. 27, 2015, 3 p.m....fluorescent protein fusions in live cells in real time. Previously, we have described a number of practical considerations... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....aliquot/preparation of the test plasmid. Let us now revisit our experiment. In Figure 1, it appeared as though... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m.... Used since the mid-90s, this technique has revolutionised the way we apprehend molecular complexes and... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....and Samie R. Jaffrey. "A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat–containing... -
Plasmids 101: Biotinylation
TypeBlog PostPublishedNov. 15, 2018, 1:50 p.m....in different microenvironments. There are also reversible and cleavable biotinylation reagents to help ... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m....2015) Vectored antibody gene delivery for the prevention or treatment of HIV infection. Current Opinion... -
The Advances Behind The World's First Personalized CRISPR Treatment
TypeBlog PostPublishedSept. 16, 2025, 1:15 p.m....mammalian cells appeared in 2013 (Cong et al., 2013; reviewed in Lander, 2016). The first base editor was reported... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....structure, function, and regulation. Biophysical Reviews, 13(6), 1081–1090. https://doi.org/10.1007/s12551... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog PostPublishedDec. 2, 2019, 2 p.m....Biotinylation Other resources Proximity labeling review: Trinkle-Mulcahy, L. (2019). Recent advances in... -
Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m.... visualized, achieved through, for example, a previously induced gene knockout. Besides amine- and DNA-binding... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....integrin antibodies discovered by yeast display reveal αV subunit pairing preferences with β subunits ... -
March for Science
TypeBlog PostPublishedApril 21, 2017, 2:30 p.m....hypothesis. Looking at the data, scientists then revise their hypotheses and the cycle begins again. No... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....deficient in dUTPase and uracil deglycosidase. dUTPase prevents the buildup of dUTP and uracil deglycosidase removes... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m....given a 50 minute talk (not a lab meeting) in the previous year. Usually fewer than 10% have done so. How... -
Site Directed Mutagenesis by PCR
TypeBlog PostPublishedAug. 2, 2016, 2:30 p.m....screening for succesfully mutated clones. Forward and reverse primers are designed to be complementary, but each... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog PostPublishedMarch 1, 2016, 3:30 p.m....Sequence and Ligation Independent Cloning (SLIC) in a previous Plasmids 101 post. Although SLIC may be more cost... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m....https://doi.org/10.5607/en20006 Rolland, A.-S., Kareva, T., Yarygina, O., Kholodilov, N., & Burke, R. ...