We narrowed to 605 results for: abo.1

...plasmids are passed down through generations (Figure 1). These so called “plasmid addiction systems” require... more about CcdB, the toxic key to efficient cloning Read all Plasmids 101 blog posts Read about knockout...with the other toxin-antitoxin systems described above, one part of the pair is more stable than the other...

...drives our bioinformatics needs       Fig. 1: QC'ing data at Addgene is like standing under a Niagara...much information as possible from depositing laboratories and associated publications, but our quality...the process. Another design choice we appreciated about Nextflow was how effortless it was for us to switch...interface. In our next post, we’ll share our story about how we used Nextflow Tower to augment our pipeline...

...antibody bonding strengths.    . Steps of an IP: 1) Protein extracts from cells or tissues contain a ...proteins or nucleic acids. Read on to learn more about this versatile technique.  Immunoprecipitation ...proteins in an IP are not denatured (learn more about antibodies here!) When using Protein A/G to immobilize...eluted by boiling the beads in SDS. (To learn more about Western blotting check out our blog post The Basics...

...antibiotic selection. Essentially, this gene is a 2-in-1 providing both bacterial and eukaryotic selection,...therefore prevent cell death. One of the cool things about amp is that it's exclusively toxic to dividing cells...antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they likely do! ...

...designed to shuttle between species. Figure 1: Shuttle vectors are vectors that can function in ...your experiment, meaning you don’t have to worry about vector replication at all. In these cases, transient...place in. In many cases, you won’t need to worry about the expression of your gene of interest in both ...

...sample’s absorption at 260 nm (A260). Figure 1: Characteristic absorption spectra for pure DNA and...flow-through solutions and use clean tubes? How about spin times, were these long enough?  There are, ...

... end sequences (R and L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon..., 2020), to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology...

...transducing your virus. Let’s start there!   Figure 1: Flowchart showing the process for using pooled lentiviral...ordering a pre-made library, you don’t need to worry about amplifying and producing lentivirus. But pre-made...

... of low-toxicity RNA editing tools.   Fig. 1 Schematic of luciferase RNA knockdown assays using.... Eugene Koonin and Kira Makarova, longtime collaborators of Omar and Jonathan and experts in CRISPR evolution...

...email Ryan at [email protected]   References 1. Larivière, Vincent, Yves Gingras, and Éric Archambault...learn about how researchers are using “big data” to look at Lyme Disease in new ways. Or learn about the...ot­her disciplines, are even less likely to read about our work. We therefore must discover new ways to.... Only on occasion do we then have time to read about research from other disciplines, or from other countries...communication through video     Video abstracts about research, especially published research, have grown...researchers have Facebook pages, few share any details about their research itself. Pinterest is also a favorite...created a new platform for sharing video abstracts about research: www.WeShareScience.org. WeShareScience...

...first foray into Reddit. Here are some reasons why: 1. Reddit has a large and active community of people...not too worried about selling yourself and really do just want to get information about yourself and your...where users can participate in forum discussions about a wide variety of topics. Individual forums are ...where users can ask designated scientists anything about a particular topic. Given Joanne’s expertise in... is well moderated. One of our original worries about sharing science advice on Reddit was that we’d be...answering questions. r/Science has pretty strict rules about not being too sales-y in posts which falls in line...any scientists to refer to if they have questions about science careers, women in STEM, work-life negotiation...

...conference for any reason was a Singleton. Figure 1: The Binning Singletons logo. Image: James et al.,...noticed Holly Bik live-tweeting about topics I needed to know more about. I caught up with her outside ...me for coffee and spent an hour with me talking about the tools I could use, and some experimental hurdles...other folks at the meeting. I didn’t know anything about these people at the time, but I met some very accomplished... my mind because we’d had discussions in my lab about whether or not to keep DNA sequencing singletons...singletons out, and there is healthy debate online about this (our lab keeps singletons). I felt like anyone...scientific conferences Read our ECR Toolbox blog post about using social media as a scientist ...

... over again. Here’s that process!   Figure 1: The Design-Build-Test-Learn cycle adapted for video... Production Specialist, I’m constantly thinking about ways to improve our productions to deliver the best...four months after I started with Addgene, I was about to have a check-in with my boss Jennifer. I had ... would be a couple months. Jennifer and I spent about an hour that morning scrambling. Everything we had...someone to be comfortable on camera while thinking about things like lighting, camera angle, and shot composition... allowing people to sit without having to worry about getting in close to mic them up. Monitor: I then...first video in our Intro to the Lab Bench series about PPE! We made this with a combination of in-lab filming...

...yourself in a creative rut, read on!   Fig 1: Blugene using creative problem-solving in the lab...brainstorming sessions.  Allow the group time to think about the problem before you meet. This holds the dual... meetings run smoother when people have thought about the issue ahead of time. Encourage dissent. The ... decisions while encouraging the group to think about alternative options. When a devil’s advocate is ...a few minutes every month dedicated to thinking about the future will improve your ability to innovate...to be able to continue our experiments. Thinking about these plans before you’ve run out of consumables...

...levels of DnaA to get the job done.   Figure 1: DNA melting by DnaA and IHF is the first step of ... negative regulators. The genome of E. coli is about 4.6 million base pairs long and contains a single...copy number and compensating for the additional metabolic cost imposed on the host with a beneficial function.... On the other hand, they also impose a lower metabolic cost on cells and produce lower amounts of protein...

...indel formation and phenotypic changes as early as 1 week post-injection. Livers from these mice were histologically...Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR enzyme Cpf1 ... and specific? The two AAV strategies described above showed successful target modification, indicating...

...complex. So how exactly does this work? Figure 1: APOBEC- and Cas9-mediated editing (ACE) reporter ...for base-editing activity The reporters mentioned above all rely on GFP fluorescence and cell sorting using... Additional resources on the Addgene blog Read about other CRISPR reporters Browse our CRISPR blog posts...

...therapies to clinical trials in 2021. Figure 1: Schematic of the human MECP2 locus and MECP2 protein...therapy. In 2014, RSRT brought together a strong collaboration between two gene therapy labs (Brian Kaspar ...

...’re not one of these people yourself.  Fig 1: Quote from Michael Ende, courtesy of morefamousquotes.com...rely on what I know about those particular teams at that given time. I think about how previous iterations...disagreements. In fact, conflict resolution isn’t just about resolving conflicts between other people, but resolving...normally get along well with each other disagree about a specific decision, direction, or goal. Resolving...of lapsed communication. Making some assumptions about how something should be communicated is necessary...confusion around... Ownership: Misunderstandings about who is responsible for what tasks or projects is...conflicts.  Transitioning a conflict into a collaboration may not be the easiest resolution to accomplish...

...30, 2018 and updated April 16, 2020. Figure 1: Comparison of SHERLOCK and DETECTR nucleic acid detection...Addgene Blog Learn about using toehold switches to detect Zika virus   Learn more about targeting RNA with...time-consuming, and complex to use outside of standard laboratories. In the case of the COVID-19 pandemic, qPCR ...with Cas13a (C2c2) Learn more about Cas12a (Cpf1) and its multiplex genome editing abilities Resources...