We narrowed to 610 results for: cas
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TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m....edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies...
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Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m.... contamination. To date, Addgene has never had a case of mycoplasma contamination. In the event of contamination... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog PostPublishedJuly 25, 2024, 1:15 p.m....process can be laborious and expensive. In some cases, conjugation methods can interfere with binding,... -
Tips for Writing a Good Cover Letter
TypeBlog PostPublishedMay 21, 2024, 1:30 p.m.... to three paragraphs. This is where you make the case for why you are a good candidate, explain how your... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....incorporate interchangeable antibiotic resistance cassettes (Prior et al. 2010), inducible promoters with ... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....the salary changes expected for postdocs in many cases should be as described in the notice of the new ... -
Getting the Most from Your Lentiviral Transduction
TypeBlog PostPublishedApril 7, 2016, 2:30 p.m....in a higher volume of lower titer virus. In such cases you may want to consider concentrating your viral... -
Tips for Using FRET in Your Experiments
TypeBlog PostPublishedNov. 5, 2014, 3:41 p.m....with the laser used to excite the donor. In that case the cross-talk is high and your background signal... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m....oftentimes put to therapeutic uses, and in those cases it's important to identify the right tissue-specific... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m.... same XFP transgene in an adult fly. As a stop cassette is located directly upstream of the fluorescent... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog PostPublishedJan. 29, 2019, 2:27 p.m....cellular RNA to specific intracellular locations using Cas9 and track the localization of the complex. Broccoli... -
March for Science
TypeBlog PostPublishedApril 21, 2017, 2:30 p.m....Information on how to contact your representatives in case you would like to talk with them about science: ... -
Transferable Skills Guide: Conflict Resolution
TypeBlog PostPublishedJan. 4, 2022, 2:15 p.m....perceive any conflict as inherently bad, and in many cases it can be. If people or teams can’t see eye to eye... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....and migration, initiating intracellular signal cascades, and allowing agile responses to mechanical forces... -
Evolution of Lab Techniques
TypeBlog PostPublishedJune 21, 2016, 2:30 p.m....detection methods are also commonly used. In all cases, the result can be quantified by the strength of... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....that means saying no to a negotiation or, in this case, to academia altogether. The primary point of ... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m....undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name calling, insults, intimidation... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m....what her cat is thinking. Find her on Twitter @JessicaSacher. References 1. Nobrega, Franklin L., et al... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes. Nat Biotechnol... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....lacking gaps or mismatched base pairs (as in the case for LCR, LDR and Padlock Probes), use of a high ...