We narrowed to 817 results for: nin
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TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....retina. This pattern was consistent with TUNEL staining, a popular method to observe apoptotic cells. ...Central PMCID: PMC5026494. Zhang, Qiang, et al. "Designing a green fluorogenic protease reporter by flipping...
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Networking Follow-up: I Met a Person. Now What?
TypeBlog PostPublishedJan. 12, 2021, 2:15 p.m....relationships Here are some ways to go about strengthening a new relationship with authenticity and not...(or better yet, become a volunteer). Naturally running into or working with the same people is often the... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog PostPublishedJuly 21, 2020, 1:15 p.m....DNA this way. First, start by pouring your gel containing a DNA intercalating dye (ex: ethidium bromide...research Browse protocols for molecular biology, cloning, and more ... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog PostPublishedFeb. 9, 2021, 2:15 p.m....to chat. This can be done randomly or manually assigning which participants to group together. Breakout...doing science, he has assisted many researchers in gaining the confidence to launch new and diverse careers... -
25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....while listening to podcasts made for kids. Plan out meals and snacks ahead of time. Find learning activities... -
Cultivating Community Science at BosLab
TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m....laboratory for citizen scientists. In addition to maintaining a laboratory to support both individual and community...that everyone gets proper equipment and safety training prior to getting started. For example, Timothy... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog PostPublishedDec. 20, 2018, 1:15 p.m....remembered kindly. However, despite many headlines questioning if He’s experiments prove otherwise, the scientific...biosecurity, and the entire 2018 saga of “what’s happening in genetics this time?” She lives with her partner... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....hour to achieve the purpose of the meeting (see “defining the scope” above) or if you can cut it down to...leader for some tips. Before long, you will be running collaborative, informative, energizing meetings... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m....When you are running flow cytometry, you’ll need various controls to help you set up and analyze your...and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C in APC with the IgG2c subclass... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....tools, where light used for excitation can cause unintended activation of the optogenetic system. Biosensors... cellular physiology in your system. Optical sectioning cannot be conducted with luminescence imaging... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog PostPublishedMay 9, 2017, 2:12 p.m....pre-crRNA arrays simplifies the crRNA cloning process. For cloning, Zetsche et al used four oligos that... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m....Institute have supported the AAV initiative from the beginning and have worked closely with us to functionally... isn’t the only facet of a project this big. Maintaining our excellent service and accuracy involves caring... -
Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m....bootstrap your science communication skills with training programs, which are increasingly available. But... draw and the perspective I want them to form? Training in science communication should prepare you to... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....manipulate what this cargo is and where it goes, turning these pesky germs into useful tools. Here, we will...Harbor, NY, USA: 1997. PubMed PMID: 21433340. 4. Cronin J, et al. “Altering the tropism of lentiviral vectors... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m....bind its target protein. Generating OptoNBs The opening and closing of the AsLOV2 domain allosterically...catalytic domain of the Sons of Sevenless (SOS), a guanine nucleotide exchange factor that activates Ras. ... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m....subject to mishandling? Is the experiment you’re planning to run with the antibodies time-sensitive, using...which you may need in your experiment. If you are planning to conjugate your antibody in the lab, you’ll ... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog PostPublishedJan. 30, 2020, 2:15 p.m....with powerful cognitive biases such as motivated reasoning and hindsight bias, we may fool ourselves and ...discoveries Finally, there are good reasons for obtaining a different result after conducting a replication... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog PostPublishedJan. 25, 2024, 2:15 p.m....you are in the design phase of your experimental planning, you need more nitty-gritty details — how can ...link to search Addgene's collection for plasmids containing that enzyme, and embedded in the References lists... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog PostPublishedDec. 11, 2018, 12:46 p.m....Developing science communication skills, while also learning about new scientific developments and helping ...s advice to tap into the online conversations happening around a scientific conference, whether you are... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....chlorophenol red-b-D-galactopyranoside on the assay paper turning it purple, allowing for easy visual detection of...Sidhu’s group has made several hundred SH3 domain containing plasmids available through Addgene. These plasmids...