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  4. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...product on a gel to analyze product size. This blog post discusses some of the key things to consider when...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ... country. Have any other questions? Feel free to post them in the comments and we would be happy to answer...

  8. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...a distinct gene delivery method. We focused this post on shuttle vectors delivered by transformation and...

  9. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...Plasmids articles.  Here's what you'll find in this post: Optimized prime editing in dicots Self-labeling...subthreshold events, including miniature excitatory postsynaptic potentials (mEPSPs) (Hao and Lee et al., 2024...Anti-APP [6E10-A5] recombinant antibody in human postmortem brain samples. Results show positive staining...

  10. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...We recently updated our blog post on Prime Editing, and that meant rereading many of the original papers...

  12. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...as part of the templated DNA. Check out our blog post on increasing HDR efficiency to read more about ...

  14. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...currently stuck trying to write one, hopefully this post will help you get things moving - remember you're...

  15. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...recombined construct. Read our recent Plasmids 101 Post on CcdB for more information. How to clone using...

  16. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...Perkins and Stephanie Hays for images used in this post. Klaus Wanisch is an Outreach Scientist at Addgene...only become obvious at rather late stages (i.e. postdoc level) and can include the pressure to publish ...become more appealing on the job market (e.g. a postgraduate degree in law, an MBA, or similar), there are...developed by life science PhDs. In my days as junior postdoc I was aware that such roles existed, but did not...

  18. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...page Read our transferable skills guide Read blog posts about career readiness Resources at Addgene.org... their lab, but for many graduate students and postdocs, the people you see in your bay for 8-12 hours...floundered for a solid year after I started my postdoc! It’s a lot easier to manage your time. As my...

  19. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...This post was contributed by Laura Lee, a graduate student at Stanford University. Arabidopsis is a fantastic...
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