We narrowed to 694 results for: ust
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TypeBlog PostPublishedFeb. 15, 2024, 2:15 p.m....supernatant through each layer. The right panel illustrates a gradient tube before and after ultracentrifugation...
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CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog PostPublishedJan. 25, 2024, 2:15 p.m....experiment. Perhaps you are a structure junky who just wants to learn about Cas domains. CasPEDIA either... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....Or maybe it’s a biosensor?! You can do more than just ‘sense’ biological compounds and reactions; you ... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog PostPublishedApril 27, 2023, 1:15 p.m....experiments with chemogenetic vectors, this property must be taken into account since activation with chemogenetic... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....If you’re just getting started using antibodies in your experiments, you may be curious about all of ... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....remove the integrated GFP reporter leaving you with just your desired genomic edit. Find SRIRACCHA plasmids... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....arises for base editors is that the editing domain must induce the base change before the Cas domain lets... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....is very little crossover. The induction mechanism must also be compatible with your experimental system... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog PostPublishedDec. 15, 2017, 2:07 p.m....help researchers use these great tools. If you’re just getting into neuroscience, you could look to our... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog PostPublishedMarch 30, 2017, 2:30 p.m....for mutation validation, we found these assays frustrating to work with, time consuming, and minimally informative... -
Targeted Mutagenesis with EvolvR
TypeBlog PostPublishedFeb. 21, 2019, 1:15 p.m....requires constant turbidity of the culture, so bacteria must be grown in a turbidostat. Only mutate particular... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog PostPublishedDec. 11, 2018, 12:46 p.m....I wrote my first blog post out of boredom. I had just started working in a new lab and I was the only ... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....its amenability to genetic manipulation. This is just a primer so that you are aware of at least some ... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m.... based on Nano-lantern technology are also very robust, with an eNL-based calcium sensor displaying a ... -
Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m....works as a data scientist in the entertainment industry. Additional resources on the Addgene Blog... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....resulting in a drop in transduction coverage compared to just one vector. Q2: Do you think it is possible to... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....any protein in all kinds of different assays, not just transcription factors. The second vector is a homology... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m....introduced. Repair of Cas9-induced breaks by NHEJ As illustrated below, NHEJ-mediated repair of Cas9-generated... -
Dos & Don'ts When Publishing a Scientific Manuscript
TypeBlog PostPublishedMay 22, 2014, 4:02 p.m....of your research may stipulate that your article must be published in an open access journal or by using... -
To Each HIS Own
TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m....Antibodies.) Unfortunately, for some proteins there just aren’t any good antibodies available. In these cases...