We narrowed to 729 results for: NSI
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TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m....space where BosLab exists today. Back then, it consisted of “wooden slab benches” set on top of filing ...companies and BosLab has grown into an organization consisting of over twenty members and volunteers. Transforming...biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA with small molecules....
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Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....applications such as super resolution, electron, and expansion microscopy. hfYFP is not only brilliantly bright...than most FPs. The group also tested hfYFP in expansion microscopy as well as correlative light and electron...stable FP for your next microscopy experiment, consider hfYFP and LSSmGFP. Fig. 4: hfYFP retains... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....almost complete desensitization when exposed to continuous bright light. Desensitization is mediated by...discovered Marine, Anion-conducting and Intensely Desensitizing ChRs) differ from currently used ChRs in that... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....caspases are activated last in the cascade and are responsible for the degradation of over 600 cellular components...detected fluorescence within 2 to 5 hours, a time consistent with previous methods. In zebrafish, the Shu ...developing forebrain and retina. This pattern was consistent with TUNEL staining, a popular method to observe... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....using CRISPR/Cas9. They are targeting the genes responsible for both early onset and late onset AD. In this...These papers suggest it might work well enough to consider as a therapeutic.” There are many fundamental ...handle on how cleavage sites are repaired.” Those insights will be critical for scientists’ ability to influence... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog PostPublishedDec. 10, 2019, 1:58 p.m....then integrates into the genome. Thus the gRNAs responsible for a phenotype can be identified by next-generation...UAS). The Wang Lab created a tripartite system consisting of dCas9, a gRNA fused to an RNA binding motif...a requirement for very high excitation light intensities have made these indicators difficult to work ... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m....found, to providing reagents in an accessible and consistent way so other researchers can use them, to making... can and do play this role, and we take our responsibility to push for clear communication of science ...broader role as Vice President. Her current responsibilities encompass overall leadership of the Cell Press... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....are there. Make the most of everyone’s time by considering the level of meeting you need. Project Meetings...meetings tend to fill the shape of their container. Consider whether you actually need a full hour to achieve...FInd Tips on Picking a Research Project Read Other Inside Addgene Posts Resources on the Addgene Website... -
Finding and Joining Your Dream Lab
TypeBlog PostPublishedApril 8, 2014, 2:50 p.m.... perhaps surprising, yet important, factors to consider when seeking your dream lab. The principal investigator's...want a non-academic career path? If you are considering this, make sure to choose an advisor that supports...where the topic of your research can make your transition easier – choose a lab that works on problems ... -
Clear and Accessible Writing
TypeBlog PostPublishedFeb. 15, 2024, 2:15 p.m....macromolecule density is equal to the buoyant force of the solution. Contaminants of lower densities are captured...did! From Addgene's very beginning, we had to consider digital accessibility for our website and online...