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  1. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...tested was constantly tracked with barcodes and spreadsheets. It was great to be in the lab doing experiments...was to fulfill contracts, my work sometimes felt rushed and the focus always seemed more on getting results...

  2. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...23934178. PubMed Central PMCID: PMC3782611. 8. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling...23503052. PubMed Central PMCID: PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "Defining and improving...

  3. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...Multiple first or corresponding author publications (published, not in preparation) A history of successful funding... meet future collaborators and get access to unpublished information and reagents. Part of the reason ...

  4. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Originally published Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan ...experimentation. In 2014, several manuscripts published in Genetics detailed approaches in which selection...

  5. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy...plasmids from this paper at Addgene. 5. Tsai, Shengdar Q., et al. “Dimeric CRISPR RNA-guided FokI nucleases...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...manufacturer and/or in the published literature. Note that the antibody being published doesn’t always indicate...

  7. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...advantage of AAV vectors is that there are well-established protocols for their production and purification...PubMed Central PMCID: PMC4324159. Castle, M.J., Gershenson, Z.T., Giles, A.R., Holzbaur, E.L., and Wolfe...

  8. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...cytometry. It's a great introduction to — or quick refresher on — these common antibody applications.  Thanks...

  9. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...is below.      And you’ll be able to find all published videos in our Quick Tips playlist:  Want your...

  10. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...processing of our sequencing data.”  Recently, Seqera published a case study on how Addgene has used Nextflow ...
Showing: 301 - 310 of 512 results